Teste de tetrazólio em sementes de crambe

Rezende, Roberta Guimarães; Jesus, Lorena Leão de; Nery, Marcela Carlota; Rocha, Adriana de Souza; Cruz, Sara Michelly; Andrade, Paulo César de Resende

doi:10.5433/1679-0359.2015v36n4p2539

Cited by 9 publications

(7 citation statements)

References 4 publications

(1 reference statement)

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“…parahyba (Fogaça et al, 2011), Annona cherimola Mill. x Annona squamosa L. (Gimenez et al, 2014), Stizolobium aterrimum (Deminicis et al, 2014) y Crambe marítima L. (Guimarães et al, 2015). Lo antes anotado, se ratificó con los resultados obtenidos en la prueba de germinación en cámara germinativa, cuyo porcentaje de plántulas germinadas (85% -95%), no presentó diferencias significativas con los porcentajes de semillas viables estimados mediante los diferentes tratamientos de la prueba bioquímica de tetrazolio (Cuadro 2).…”

Section: Evaluación De Diferentes Concentraciones De Tetrazolio Y Tieunclassified

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Camacho

Tatis²,

Ayala³

2017

Mesoam. Agron.

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© 2017 Agronomía Mesoamericana es desarrollada en la Universidad de Costa Rica y se encuentra licenciada con Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 Costa Rica. Para más información escríbanos a pccmca@ucr.ac.cr ResumenEl objetivo del estudio fue describir las características morfométricas, anatómicas y la viabilidad de las semillas de Cedrela odorata y Cariniana pyriformis. El trabajo se llevó a cabo en la Universidad de Córdoba de Montería, Colombia, en el período comprendido entre abril de 2012 a julio de 2014; con una metodología descriptiva de laboratorio, la prueba de tetrazolio y la prueba de germinación en cámara germinativa. Se utilizaron semillas de lotes comerciales de tres localidades del departamento de Córdoba. Se seleccionaron al azar cinco árboles de cada especie y se tomaron al azar cinco muestras de cien semillas de cada uno. La descripción de la anatomía se hizo con base en diez semillas de cada especie. Para la prueba de tetrazolio, se estableció un experimento bajo un diseño completamente al azar, con seis tratamientos y cuatro repeticiones de veinticinco semillas. Los seis tratamientos se originaron al combinar las concentraciones de tetrazolio de 0,5, 1,0 y 1,5%, con dos y tres horas de inmersión en la solución. Las características externas de C. odorata presentaron menor variación que las de C. pyriformis, especialmente aquellas relacionadas con el peso. Cuatro patrones topológicos fueron identificados en las semillas de cada especie. Se pudo determinar la viabilidad para C. pyriformis y C. odorata, con inmersiones por dos horas en concentraciones de 0,5 y 1,0% de tetrazolio, respectivamente.Palabras clave: calidad de semilla, germinación de semillas, prueba de tetrazolio. AbstractThe aim of the study was to identify morphometric, anatomical, and the viability characteristics of the seeds of Cedrela odorata and Cariniana pyriformis. The study was carried out at the Universidad de Córdoba of Montería, Colombia, from April 2012 to July 2014; the study was carried out with a laboratory descriptive methodology, tetrazolium and germination tests in germination chamber were performed. Seeds of commercial lots from three locations in the department of Cordoba, were used. Five trees of each specie were randomly selected and from each tree, five samples of one hundred seeds, were randomly selected. The description of the shape and the anatomy of the seeds was made according to ten seeds of each specie. For the tetrazolium tests, a completely randomized design experiment, with six treatments, and four replications of 25 seeds, was established. The six treatments were originated

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Section: Evaluación De Diferentes Concentraciones De Tetrazolio Y Tieunclassified

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Camacho

Tatis²,

Ayala³

2017

Mesoam. Agron.

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“…Although the tetrazolium test is not so recent its use is still limited, which can be attributed to the need to develop procedures suitable for each species (Rezende et al, 2015). For this reason, research has been developed in an attempt to define adequate procedures for several forest species, according to Fogaça et al (2011) with Copaifera langsdorffii Desf.…”

Section: Introductionmentioning

confidence: 99%

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

et al. 2017

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Rapid tests have been essential to evaluate the physiological potential of seeds and the tetrazolium test is one of those which have been used by seed companies. The objective of the study was to establish the procedure for the tetrazolium test in Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea seeds. For this, three tetrazolium solution concentrations (0.05, 0.075 and 0.1%) and three staining periods (1, 3 and 6 hours) at temperatures of 35 to 40 °C were tested. The seeds were also evaluated by the germination test, whose result was compared with viability by the tetrazolium test. The experimental design was completely randomized in a factorial design 3 x 3 + 1 (three concentrations x three periods + a control = germination test) for each temperature. Viable seeds in the tetrazolium test were compared by Tukey’s test (p ≤ 0.05), while the comparison between the viable seeds with germination test was carried out by Dunnett’s test (p ≤ 0.05). It is recommended that the tetrazolium test for L. ferrea is carried out at a concentration of 0.05% for three-hour staining under 35 °C or 40 °C.

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“…Further studies for the adequacy of the methodology are necessary to obtain satisfactory results as those obtained for other crops, such as soybean, in which it is a standard test, extremely sensitive, and used regularly. Other species also have their appropriate methodologies, such as sunflower (SILVA et al, 2013b), cowpea (RODRIGUES et al, 2015), and crambe (REZENDE et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Physiological quality of mechanically harvested chickpea seeds

Dias

Queiroz

Castro

et al. 2021

Reveng

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Our objective was to evaluate the occurrence of mechanical damage in chickpea seeds with the combination of two harvester speeds (2.5 and 3.5 km.h-1) and three adjustments for rotor rotation speeds (500, 700, and 850 rpm). Harvesting was carried out in a seed production field. Seeds were evaluated for purity, germination (G), first count (FC), germination speed index (GSI), hypochlorite, electrical conductivity, and tetrazolium tests. There was an effect of harvester speeds on seed physiological quality for the first count (FC), germination (G), germination speed index (GSI), especially when combining with high rotor rotation speed. In these cases, the speed of 2.5 km.h-1 resulted in lower values. This harvester speed also had worse results when combining with 850 rpm for purity and hypochlorite tests. The tetrazolium test was not efficient in identifying differences in seed quality. There was no significant interaction between harvester speeds and rotor rotation speeds for the conductivity test. Evaluating the harvester speed’s isolated effect (3.5 km.h-1), we identified problems in seed vigor due to the higher value of exudates in the electrical conductivity test. Low harvester speed (2.5 km.h-1) associated with high rotor rotation speeds (700 and 850 rpm) causes a reduction of the physical and physiological quality of seeds.

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Teste de tetrazólio em sementes de crambe

Cited by 9 publications

References 4 publications

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

Physiological quality of mechanically harvested chickpea seeds

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