Abstract. We introduce two families of ideals, F -jumping ideals and F -Jacobian ideals, in order to study the singularities of hypersurfaces in positive characteristic. Both families are defined using the D-modules M α that were introduced by Blickle, Mustaţȃ and Smith. Using strong connections between F -jumping ideals and generalized test ideals, we give a characterization of F -jumping numbers for hypersurfaces. Furthermore, we give an algorithm that determines whether certain numbers are F -jumping numbers. In addition, we use F -Jacobian ideals to study intrinsic properties of the singularities of hypersurfaces. In particular, we give conditions for F -regularity. Moreover, F -Jacobian ideals behave similarly to Jacobian ideals of polynomials. Using techniques developed to study these two new families of ideals, we provide relations among test ideals, generalized test ideals, and generalized Lyubeznik numbers for hypersurfaces.