Tessaracoccus aquimaris sp. nov., isolated from the intestine of a Korean rockfish, Sebastes schlegelii, from a marine aquaculture pond

Abstract

A novel coccus-shaped, Gram-stain-positive, non-motile and aerobic bacterium, designated strain NSG39, was isolated from the intestine of a Korean rockfish, Sebastes schlegelii. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the newly isolated strain NSG39 was closely related to Tessaracoccus flavus RP1 (98.0 %). The isolate grew at 15-37 °C, pH 7-9 and 0-4 % (w/v) salinity, with optimal growth at 30 °C, pH 8 and 0 % (w/v) salinity. The cell wall of the organism contained ll-diaminopimelic acid as a diagnostic diamino acid, and ribose, mannose, glucose and galactose as diagnostic sugars. The polar lipid comprised diphosphatidylglycerol, phosphatidylglycerol, three glycolipids and four unidentified polar lipids. The major cellular fatty acid was anteiso-C15 : 0 (47.2 %). The major menaquinone was MK-9 (H4). The DNA G+C content of the isolate was 68.8 mol%. The genome-based orthologous average nucleotide identity value for strain NSG39 and T. flavus RP1 was 76.6 %. Based on the phylogenetic analysis and its biological characteristics, strain NSG39 is considered to represent a novel species of the genus Tessaracoccus, for which the name Tessaracoccus aquimaris is proposed. The type strain is NSG39 (=KACC 17540=JCM 19289).

Tessaracoccus antarcticus sp. nov., a rhodopsin-containing bacterium from an Antarctic environment and emended description of the genus Tessaracoccus

“…The draft genome sequence of strain JDX10 T consisted of 13 contigs (>1000 bp) of 3, 649,759 bp with shortest contig size of 1011 bp and longest contig size of 1, 639, 251 bp. From the draft genome sequence, the G+C content of strain JDX10 T was calculated to be 66.9 mol%, which is in accordance with reported values for Tessaracoccus species (66.7-74.5 mol%) [1][2][3][4][5][6][7][8][9][10][11]. The related genome data for strain JDX10 T and closely related type strains are listed in Table S1, available in the online version of this article.…”

“…Moreover, the predominant cellular fatty acid component is anteiso-C 15 : 0 . Species of the genus Tessaracoccus have a high DNA G+C content, ranging from 66.7 to 74.5 mol% [1][2][3][4][5][6][7][8][9][10][11]. The increasing availability of molecular identification methods has provided microbiologists with ever-increasing opportunities to identify novel species, driving an important expansion in bacterial taxonomy.…”

Tessaracoccus coleopterorum sp. nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus

“…S5). The PG and DPG in polar lipid of the isolate was same to those of type strain of T. flavescens, T. aquimaris and T. bendigoensis, but differed with those of type strain of T. defluvii containing DPG and PE, not PG [1,5,7,8]. Isoprenoid quinones were extracted according to the protocol of Collins and Jones [46].…”

“…ʟʟ-Diaminopimelic acid is the diagnostic cell wall peptidoglycan amino acid, MK-9 or MK-10 is the major isoprenoid quinone, and anteiso-C 15 : 0 is the major fatty acid; the genomic DNA G+C content is 66.7-74.5 mol% [1,[3][4][5][6][7][8][9][10][11][12][13]. Tessaracoccus species have been retrieved from marine environments (marine sediment and sea sand) [6,8], soils (oil-contaminated soils and Antarctican soil) [3,4,13], industrial environments (drainage system of a lindane-producing factory, deep subsurface of the pyrite belt, and metal working fluid) [9][10][11], animals (rockfish and rhinoceros) [5,12], activated sludge biomass [1], and sewage treatment plants [7]. Insect guts harbour a large variety of microbes that affect host physiology including development, nutrition, metabolism, immunity, defence, behaviour, and detoxification [14][15][16][17][18].…”

“…To perform a more comprehensive and comparative characterization of the isolate, the type strains of the closest relatives T. flavescens KCTC 19196 T , T. defluvii JCM 17540 T , and T. aquimaris NSG39 T , and the type species of the genus Tessaracoccus (T. bendigoensis JCM 13525 T ) were used as reference strains. All reference strains were obtained from KCTC or JCM, except T. aquimaris NSG39 T , which is the species described in our previous report [5]. The biochemical characteristics, i.e.…”