Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain

Cano-Gómez, Cristina; Garcia-Casado, Maria; Soriguer, Ramón C.; Palero, Ferran; Jiménez‐Clavero, Miguel Ángel

doi:10.1016/j.vetmic.2012.11.022

Cited by 32 publications

(20 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study performed in Spain reported that PTV was more frequently detected than PSV and, as it was observed in the results of the present study, single infections by PSV were not identified (Cano-Gómez et al 2013). Despite some differences in the PCR techniques, protocols, and ages of the evaluated animals in the two previous reports (Prodělalová 2012, Cano-Gómez et al 2013 and in this study, the results are based on molecular assays that target the 5'-NTR region of the porcine enteric picornavirus' RNA.…”

Section: Discussionsupporting

confidence: 71%

“…In contrast, a Brazilian study reported the 3 viruses in post--weaning pigs (4-8 weeks old) but did not detect PSV in suckling piglets (1-3 weeks old) (Donin et al 2014). Co--infections with 2 or 3 porcine enteric picornaviruses were common in the different age groups (Prodělalová 2012, Cano-Gómez et al 2013, Donin et al 2014.…”

Section: Discussionmentioning

confidence: 81%

“…Despite some differences in the PCR techniques, protocols, and ages of the evaluated animals in the two previous reports (Prodělalová 2012, Cano-Gómez et al 2013 and in this study, the results are based on molecular assays that target the 5'-NTR region of the porcine enteric picornavirus' RNA. The similarities among the results presented herein, in the Czech and Spanish studies may indicate that wild boars are more susceptible to infection by EV-G and PTV.…”

Section: Discussionmentioning

confidence: 97%

See 2 more Smart Citations

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

et al. 2015

View full text Add to dashboard Cite

RESUMO.-[Estudo molecular de teschovírus suíno, sapelovírus suíno e enterovírus G em javalis (Sus scrofa scrofa) de cativeiro no Paraná.] Teschovírus suíno (PTV), sapelovírus suíno (PSV) e enterovírus G (EV-G) são agentes infecciosos específicos da espécie suína que estão endemicamente disseminados em todo o mundo. O objetivo deste estudo foi investigar a infecção natural por estes picornavírus entéricos suínos em javalis (Sus scrofa scrofa) do estado do Paraná, Brasil e avaliar pecaris (Pecari tajacu e Tayassu pecari) como hospedeiros alternativos para estes vírus. Amostras fecais (n=36) de javalis assintomáticos (n=22) com idades de 2 a 7 meses (jovens, n=14) e 2 a 4 anos (adultos, n=8) e de pecaris (6 a 8 meses de idade, n=14) foram coletadas em um cativeiro e zoológico, res- Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric picornaviruses in wild boars (Sus scrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5'non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87

show abstract

Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Antibodies against transmissible gastroenteritis virus (TGEV) have been detected extremely rarely (Hälli et al, 2012;McGregor et al, 2015). Viral nucleic acids of porcine enteric picornaviruses, comprising teschoviruses, sapeloviruses, and enteroviruses G have been detected in stool samples (Prod elalová, 2012;Cano-Goméz et al, 2013). Moreover, wild boars represent an important source of zoonotic hepatitis E virus (Schlosser et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological survey of enteric viruses in wild boars in the Czech Republic: First evidence of close relationship between wild boar and human rotavirus A strains

Moutelíková

Dufková

Kamler

et al. 2016

Veterinary Microbiology

View full text Add to dashboard Cite

Population of wild boar is increasing in the whole Europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. The aim of the study was to estimate in population of free-living wild boar in the Czech Republic the prevalence of enteric viral pathogens, namely rotavirus groups A and C (RVA and RVC), porcine reproductive and respiratory syndrome virus (PRRSV), and members of family Coronaviridae (transmissible gastroenteritis virus - TGEV, porcine epidemic diarrhea virus - PEDV, porcine respiratory coronavirus - PRCV, and porcine hemagglutination encephalomyelitis virus - PHEV) and Picornaviridae,(teschovirus A - PTV, sapelovirus A - PSV, and enterovirus G - EV-G). In our study, stool samples from 203 wild boars culled during hunting season 2014-2015 (from October to January) were examined by RT-PCR. RVA was detected in 2.5% of tested samples. Nucleotide analysis of VP7, VP4, and VP6 genes revealed that four RVA strains belong to G4P[25]I1, G4P[6]I5, G11P[13]I5, and G5P[13]I5 genotypes and phylogenetic analysis suggested close relation to porcine and human RVAs. The prevalence of RVC in wild boar population reached 12.8%, PTV was detected in 20.2%, PSV in 8.9%, and EV-G in 2.5% of samples. During our study no PRRSV or coronaviruses were detected. Our study provides the first evidence of RVC prevalence in wild boars and indicates that wild boars might contribute to the genetic variability of RVA and also serve as an important reservoir of other enteric viruses.

show abstract

“…The phylogenetic relationships between the PTV detected and the reference strains are showed in a phylogenetic tree (Figure ). This figure shows the main clusters corresponding to different serotypes of PTV, 11 corresponding to the fully recognized PTV1‐PTV11 and two corresponding to the recently described PTV serotype 12 (Cano‐Gómez, García‐Casado, Soriguer, Palero, & Jiménez‐Clavero, ) and PTV serotype 13 (Boros et al., ; Sun et al., ).…”

mentioning

confidence: 95%

Detection of Teschovirus type 13 from two swine herds exhibiting nervous clinical signs in growing pigs

Carnero

Prieto

Polledo

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

Recently, the number of clinical reports of growing pigs showing neurological signs possibly related to viral infections has increased. The objective of this report was to describe two outbreaks of an atypical condition observed in 6- to 7-week-old pigs with a morbidity of 20% and a fatality rate of 60% in two unrelated farms of the same company. During the acute phase of the disease, fever, sudden death, neurological signs, ear necrosis and occasional corneal opacity were observed. Histopathological examination revealed interstitial pneumonia, lymphoid depletion and lymphocytic vasculitis in different organs and mild polioencephalomyelitis suggesting a potential viral infection. Possible aetiologies such as exogenous intoxications, salt intoxication, mineral deficiencies/intoxications (Se, Cu, Cd and Zn), oedema disease and mycotoxicosis were ruled out through the diagnostic process. No clinically relevant bacteria could be consistently isolated from affected animals, and the presence of the common swine viruses was ruled out by PCR or RT-PCR. Porcine Teschovirus serotype 13 was the only virus detected by RT-PCR within central nervous system (CNS) of acutely affected pigs. This is the first description of PTV serotype 13 within the CNS of clinically affected pigs.

show abstract

Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain

Cited by 32 publications

References 34 publications

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

Epidemiological survey of enteric viruses in wild boars in the Czech Republic: First evidence of close relationship between wild boar and human rotavirus A strains

Detection of Teschovirus type 13 from two swine herds exhibiting nervous clinical signs in growing pigs

Contact Info

Product

Resources

About