Terrestrosin D, a Steroidal Saponin from &lt;b&gt;&lt;i&gt;Tribulus terrestris&lt;/i&gt;&lt;/b&gt; L., Inhibits Growth and Angiogenesis of Human Prostate Cancer in vitro and in vivo

Wei, Shihu; Fukuhara, Hideo; Chen, Guang; Kawada, Chiaki; Kurabayashi, Atsushi; Furihata, Mutsuo; Inoue, Keiji; Shuin, Taro

doi:10.1159/000357622

Cited by 37 publications

(26 citation statements)

References 23 publications

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“…Terrestrosin D isolated from T. terrestris effectively inhibited viability of HUVEC cells and also induced cell cycle arrest and apoptosis in the cells, which suggests its antiangiogenic potential in vitro (Wei et al 2014). Similar observations were made for ASC…”

Section: Angiogenesis Inhibitionsupporting

confidence: 75%

“…, isolated from T. terrestris, significantly decreased the viability of androgen-independent (DU-145, PC-3, PC-3M) and androgen-dependent (LNCaP, 22RV1) human prostate cancer cells, in dose-dependent manner (Wei et al 2014). Moreover, the compound induced PC-3 cell cycle arrest in G1 phase and stimulated caspase-independent apoptosis in the cells.…”

Section: Results Of In Vitro Studiesmentioning

confidence: 94%

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Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

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Steroidal saponins are a group of glycosides widely distributed among monocotyledonous families. They exert a wide spectrum of biological effects including cytotoxic and antitumor properties which are the most studied. This review is an update of our previous paper-Saponins as cytotoxic agents (Podolak et al. in Phytochem Rev 9:425-474, 2010) and covers studies that were since published (2010-2018). In this paper we refer to steroidal saponins presenting results of cytotoxicity studies, mechanisms of action and structure-activity relationships.

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Section: Angiogenesis Inhibitionsupporting

confidence: 75%

Section: Results Of In Vitro Studiesmentioning

confidence: 94%

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

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“…Cell proliferation was determined by 3-(4, 5-dimethyl-thiogol-2-yl)-2, 5-diplenyltetrazolium (MTT) assay, as we previously reported [14]. Colony formation was assessed as described [15].…”

Section: Methodsmentioning

confidence: 99%

Silencing of ATPase Inhibitory Factor 1 Inhibits Cell Growth via Cell Cycle Arrest in Bladder Cancer

Wei¹,

Fukuhara²,

Kawada³

et al. 2015

Pathobiology

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Objective: The role of the ATPase inhibitory factor 1 (IF1) is inhibit the hydrolase activity of F₁F_o-ATPase when oxidative phosphorylation is impaired. It has been demonstrated that IF1 is overexpressed in various carcinomas and mediates tumor cell activities, but the detailed mechanisms of IF1-mediated tumor progression and the link between IF1 and cell cycle progression remain unclear. Herein, we aimed to investigate the potential role of IF1 in cell cycle progression of human bladder cancer (BCa). Methods: The expression of IF1 was analyzed by immunohistochemistry in tumor tissues. Western blot was used to detect protein expression in the cells. Cell proliferation was determined by MTT and colony formation assays. The cell cycle was analyzed using flow cytometry. Results: We firstly showed IF1 was overexpressed in BCa. Silencing of IF1 by small interfering RNA led to a significant decrease in cell proliferation and migration in T24 and UM-UC-3 cells. Importantly, IF1 knockdown caused cell cycle arrest at G₀/G₁ stage and decreased the protein level of cyclin E/cyclin-dependent kinases (cdk) 2 and/or cyclin D/cdk4/cdk6. Conclusion: These results suggest the inhibitory effect of IF1 knockdown on BCa cell proliferation is via the suppression of cyclins and cdks related to G₁/S transition and then induction of G₀/G₁ arrest, and firstly indicate IF1 mediates the tumor cell cycle. We concluded that IF1 may be a novel therapeutic target for BCa.

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“…T. terrestris extracts also induce cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells, and exhibits weak cytotoxic effects to normal cells compared to cancer cells [11]. Recently, terrestrosin D a major steroidal saponin of T. terrestris has been reported to have an antiangiogenic property, which may suggest that its anticancer effect is also due to inhibition of tumor angiogenesis [12]. It also possess anti-atherosclerotic [13], antiarthritic [14], anti-oxidant [15], antimicrobial [16], analgesic and anti-inflammatory properties [17].…”

Section: Introductionmentioning

confidence: 99%