Terrestrial eDNA survey outperforms conventional approach for detecting an invasive pest insect within an agricultural ecosystem

Allen, Michael C.; Nielsen, Anne L.; Peterson, Donnie L.; Lockwood, Julie L.

doi:10.1002/edn3.231

Cited by 28 publications

(32 citation statements)

References 59 publications

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“…The positive detection rate of the pest and its biological control predators was 2.8–4.5 times higher using eDNA methods than conventional methods (Tables 2 and 3). A similar increase in detection sensitivity has been reported in a previous study detecting invasive spotted lanternflies in vineyards (Allen et al, 2021). Furthermore, the visual inspections in this study were considerably time‐consuming with a total of 58.2 m of eastern hemlock and 234.9 m of western hemlock foliage being visually inspected.…”

Section: Discussionsupporting

confidence: 87%

“…In the case of A. tsugae detection, the eDNA was detected on all 16 trees, while visual inspection detected them on only 6 trees. A similar increase in sensitivity using eDNA detection of another terrestrial insect pest, the spotted lanternfly, has been reported (Allen et al, 2021). Visual inspection methods can be limited spatially and temporally, while eDNA can persist over time and be transported easily through space.…”

Section: Discussionsupporting

confidence: 70%

“…argenticollis. A recent study investigating a similar correlation with spotted lanternflies in grape vineyards found that 71% of the variation in visual observations could be explained by their eDNA signal (Allen et al, 2021). Determining a more precise level of A. tsugae infestations could greatly enhance the utility of eDNA data generated from surveys and in turn aid in better management decisions.…”

Section: Interpreting Positive Detection Rates Of Edna and Traditiona...mentioning

confidence: 91%

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Sensitive environmental DNA (eDNA) methods to detect hemlock woolly adelgid and its biological control predators Leucotaraxis silver flies and a Laricobius beetle

et al. 2022

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Environmental DNA (eDNA) analysis can be a powerful tool for the early detection of invasive organisms. However, research on terrestrial eDNA detection from foliage surfaces has been limited. In this study, we developed methods to capture and detect eDNA using qPCR from an invasive forest pest, hemlock woolly adelgid (Adelges tsugae), and three of its biological control predators Leucotaraxis piniperda, Leucotaraxis argenticollis, and Laricobius nigrinus. We designed four highly efficient qPCR assays with a low limit of detection (1-10 copies/reaction). The assay targeting A. tsugae was species-specific. The assays targeting Le. piniperda, and Le. argenticollis were biotype-specific in addition to being species-specific demonstrating applications of eDNA analysis beyond species-level detection. The La. nigrinus assay also detected DNA from closely related and hybridizing Laricobius rubidus. The eDNA methods were evaluated against traditional detection methods. We collected foliage samples from three strata (bottom, middle, and top) of eastern hemlock trees to detect the presence of A. tsugae. The detection of the biological control predators was evaluated using western hemlock foliage samples collected from the predators' native range in western Washington. The eDNA methods had significantly higher positive detection rates (2.8-4.5 times) than conventional methods of all target species. The strata of sampling were not significant in determining the presence of A. tsugae infestation.The eDNA concentration positively correlated with the observed density for all species. This study demonstrates the efficacy of eDNA analysis as a more sensitive tool for early detection of A. tsugae and to track the establishment of its biological control predators.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 70%

Section: Interpreting Positive Detection Rates Of Edna and Traditiona...mentioning

confidence: 91%

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Sensitive environmental DNA (eDNA) methods to detect hemlock woolly adelgid and its biological control predators Leucotaraxis silver flies and a Laricobius beetle

et al. 2022

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“…Though terrestrial ecosystems can be seen as the depository of past biodiversity exploited to reconstruct past ecosystems [ 35 ], the modern and historical signals conveyed by the soil can be difficult to disentangle, leading to a higher risk of false positive detection for contemporary species [ 36 , 37 ]. However, one should be mindful that the total soil eDNA usually corresponds to the current material, as proven by the study carried out by Zinger et al (2009), studying seasonal shifts of communities using soil eDNA.…”

Section: Persistence Detectability and Mobility Of Terrestrial Ednamentioning

confidence: 99%

Environmental DNA Metabarcoding: A Novel Contrivance for Documenting Terrestrial Biodiversity

Hassan

Sabreena

Poczai

et al. 2022

Biology

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The dearth of cardinal data on species presence, dispersion, abundance, and habitat prerequisites, besides the threats impeded by escalating human pressure has enormously affected biodiversity conservation. The innovative concept of eDNA, has been introduced as a way of overcoming many of the difficulties of rigorous conventional investigations, and is hence becoming a prominent and novel method for assessing biodiversity. Recently the demand for eDNA in ecology and conservation has expanded exceedingly, despite the lack of coordinated development in appreciation of its strengths and limitations. Therefore it is pertinent and indispensable to evaluate the extent and significance of eDNA-based investigations in terrestrial habitats and to classify and recognize the critical considerations that need to be accounted before using such an approach. Presented here is a brief review to summarize the prospects and constraints of utilizing eDNA in terrestrial ecosystems, which has not been explored and exploited in greater depth and detail in such ecosystems. Given these obstacles, we focused primarily on compiling the most current research findings from journals accessible in eDNA analysis that discuss terrestrial ecosystems (2012–2022). In the current evaluation, we also review advancements and limitations related to the eDNA technique.

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“…

Recently, environmental DNA (eDNA) has been suggested as an alternative to traditional arthropod monitoring [4]. eDNA analysis has revolutionized the field of biomonitoring [5], and various substrates have been shown to contain arthropod eDNA [6]. A particularly promising source is the surface of plant material, on which arthropods deposit eDNA, for example in chew marks or

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mentioning

confidence: 99%

The bug in a teacup—monitoring arthropod–plant associations with environmental DNA from dried plant material

et al. 2022

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Environmental DNA analysis (eDNA) has revolutionized the field of biomonitoring in the past years. Various sources have been shown to contain eDNA of diverse organisms, for example, water, soil, gut content and plant surfaces. Here we show that dried plant material is a highly promising source for arthropod community eDNA. We designed a metabarcoding assay to enrich diverse arthropod communities while preventing amplification of plant DNA. Using this assay, we analysed various commercially produced teas and herbs. These samples recovered ecologically and taxonomically diverse arthropod communities, a total of over a thousand species in more than 20 orders, many of them specific to their host plant and its geographical origin. Atypically for eDNA, arthropod DNA in dried plants shows very high temporal stability, opening up plant archives as a source for historical arthropod eDNA. Considering these results, dried plant material appears excellently suited as a novel tool to monitor arthropods and arthropod–plant interactions, detect agricultural pests and identify the geographical origin of imported plant material. The simplicity of our approach and the ability to detect highly diverse arthropod communities from all over the world in tea bags also highlights its utility for outreach purposes and to raise awareness about biodiversity.

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Terrestrial eDNA survey outperforms conventional approach for detecting an invasive pest insect within an agricultural ecosystem

Cited by 28 publications

References 59 publications

Sensitive environmental DNA (eDNA) methods to detect hemlock woolly adelgid and its biological control predators Leucotaraxis silver flies and a Laricobius beetle

Sensitive environmental DNA (eDNA) methods to detect hemlock woolly adelgid and its biological control predators Leucotaraxis silver flies and a Laricobius beetle

Environmental DNA Metabarcoding: A Novel Contrivance for Documenting Terrestrial Biodiversity

The bug in a teacup—monitoring arthropod–plant associations with environmental DNA from dried plant material

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