Terpinene and β-pinene acting as signaling molecules to improve Cinnamomum camphora thermotolerance

Tian, Zhengfeng; Luo, Qian; Yan, Li; Zuo, Zhaojiang

doi:10.1016/j.indcrop.2020.112641

Cited by 17 publications

(21 citation statements)

References 67 publications

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“…accumulation (Zuo et al, 2017;Tian et al, 2020). In this study, the activities of SOD and POD also increased in the 4 chemotypes of C. camphora in the treatment with Fos+38°C.…”

Section: Discussionsupporting

confidence: 59%

“…When A. thaliana and tobacco (Nicotiana tabacum) were transferred into ISPS to become isoprene emitters, altered expression was found in the genes related with signaling networks and growth regulators, and upregulated expression was found in the genes related with stress tolerance (Zuo et al, 2019). These findings demonstrate that monoterpenes and isoprene should play important signaling roles in plant tolerating stresses (Lantz et al, 2019;Zuo et al, 2019;Tian et al, 2020).…”

Section: Introductionmentioning

confidence: 89%

“…H 2 O 2 content was estimated following the method described by Tian et al (2020). The leaf samples (0.5 g) were homogenized with cold acetone, and centrifuged at 12000 g at 4°C.…”

Section: Determination Of Ros Contentmentioning

confidence: 99%

“…Total RNA were extracted from the 3 rd and 4 th leaves from the branch with an extraction kit. They were reverse transcribed into cDNA with reverse transcriptase (RNaseH) and random hexamer primer, according to the method described by Tian et al (2020). After amplification through polymerase chain reaction (PCR), the cDNA from each sample (branch) was constructed a library for the sequence analysis in Novogene Bioinformatics Technology Co. (Beijing, China).…”

Section: Transcriptome Analysismentioning

confidence: 99%

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The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions

Wang

Luo

et al. 2022

Front. Plant Sci.

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Terpenes serve important functions in enhancing plant thermotolerance. Cinnamomum camphora mainly has eucalyptol (EuL), camphor (CmR), linalool (LnL) and borneol (BeL) chemotypes basing on the uppermost monoterpenes. To reveal the thermotolerance mechanisms of these uppermost monoterpenes (eucalyptol, camphor, linalool, and borneol) in C. camphora, we surveyed the ROS metabolism and photosynthesis in the 4 chemotypes fumigated with the corresponding uppermost monoterpene after fosmidomycin (Fos) inhibiting monoterpene synthesis under high temperature at 38°C (Fos+38°C+monoterpene), and investigated the related gene expression in EuL and CmR. Meanwhile, the thermotolerance differences among the 4 uppermost monoterpenes were analyzed. In contrast to normal temperature (28°C), ROS levels and antioxidant enzyme activities in the 4 chemotypes increased under 38°C, and further increased in the treatment with Fos inhibiting monoterpene synthesis at 38°C (Fos+38°C), which may be caused by the alterations in expression of the genes related with non-enzymatic and enzymatic antioxidant formation according to the analyses in EuL and CmR. Compared with Fos+38°C treatment, Fos+38°C+monoterpene treatments lowered ROS levels and antioxidant enzyme activities for the increased non-enzymatic antioxidant gene expression and decreased enzymatic antioxidant gene expression, respectively. High temperature at 38°C reduced the chlorophyll and carotenoid content as well as photosynthetic abilities, which may result from the declined expression of the genes associated with photosynthetic pigment biosynthesis, light reaction, and carbon fixation. Fos+38°C treatment aggravated the reduction. In contrast to Fos+38°C treatment, Fos+38°C+monoterpene treatments increased photosynthetic pigment content and improved photosynthetic abilities by up-regulating related gene expression. Among the 4 uppermost monoterpenes, camphor showed strong abilities in lowering ROS and maintaining photosynthesis, while eucalyptol showed weak abilities. This was consistent with the recovery effects of the gene expression in the treatments with camphor and eucalyptol fumigation. Therefore, the uppermost monoterpenes can enhance C. camphora thermotolerance as signaling molecules, and may have differences in the signaling functions.

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“…accumulation (Zuo et al, 2017;Tian et al, 2020). In this study, the activities of SOD and POD also increased in the 4 chemotypes of C. camphora in the treatment with Fos+38°C.…”

Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 89%

“…H 2 O 2 content was estimated following the method described by Tian et al (2020). The leaf samples (0.5 g) were homogenized with cold acetone, and centrifuged at 12000 g at 4°C.…”

Section: Determination Of Ros Contentmentioning

confidence: 99%

Section: Transcriptome Analysismentioning

confidence: 99%

See 2 more Smart Citations

The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions

Wang

Luo

et al. 2022

Front. Plant Sci.

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“…The four chemotypes were identified in a previous analysis of their volatile organic compounds (VOCs). 42,43 In each chemotype, four plants were randomly selected from more than 40 plants for leaf collection. There was a replicate for each plant.…”

Section: Preparation Of Cinnamomum Camphora Extractsmentioning

confidence: 99%

Terpenoid composition and antioxidant activity of extracts from four chemotypes of Cinnamomum camphora and their main antioxidant agents

Luo

Tian

Zheng

et al. 2021

Biofuels Bioprod Bioref

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Cinnamomum camphora (L.) J. Presl can produce an abundance of terpenoids, and has four main chemotypes: linalool, eucalyptol, camphor, and borneol. Antioxidant activity and the chemical composition of extracts from the four chemotypes were investigated during spring (May), summer (July), and autumn (November) with the aim of developing natural antioxidants using suitable chemotypes of C. camphora in suitable seasons, together with their main terpenoids. The main antioxidant terpenoids were identified. The four chemotype extracts showed strong scavenging activity against 2,2-diphenyl-1picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radicals as well as O 2 -• and •OH in July, due to the fact that their terpenoid content was at its highest during this month. Among the four chemotypes, the strongest scavenging activity was mainly detected in the linalool chemotype extracts, which had the highest terpenoid content. Among the ten main terpenoids in C. camphora extracts, ocimene, linalool, β-pinene, longifolene and eucalyptol showed stronger scavenging activity against DPPH free radicals, and ocimene exhibited the strongest scavenging activity. Extracts from the four chemotypes of C. camphora and their main terpenoids have the potential for development as natural antioxidants, with summer as the best season, linalool chemotypes as the optimum choice, and ocimene as the optimum compound.

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Monoterpene emissions and their protection effects on adult Cinnamomum camphora against high temperature

Tian

et al. 2021

Trees

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Terpinene and β-pinene acting as signaling molecules to improve Cinnamomum camphora thermotolerance

Cited by 17 publications

References 67 publications

The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions

The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions

Terpenoid composition and antioxidant activity of extracts from four chemotypes of Cinnamomum camphora and their main antioxidant agents

Monoterpene emissions and their protection effects on adult Cinnamomum camphora against high temperature

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