Terpinen-4-ol Enhances Disease Resistance of Postharvest Strawberry Fruit More Effectively than Tea Tree Oil by Activating the Phenylpropanoid Metabolism Pathway

Li, Zhenbiao; Wang, Nan; Wei, Yingying; Zou, Xiurong; Jiang, Shu; Xu, Feng; Wang, Hongfei; Shao, Xingfeng

doi:10.1021/acs.jafc.0c01840

Cited by 51 publications

(44 citation statements)

References 50 publications

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“…The PAL, the key enzyme of phenylpropanoid pathway, its activity in A. alternata-inoculated blueberries with ethanol treatment was significantly positively correlated with CHI and GLU, which indicated that ethanol vapor inducers could stimulate defense mechanisms, such as phenylpropanoid pathway activation, helping to protect the blueberries against A. alternate. Similar result was confirmed on strawberries treated by terpinen-4-ol (Li et al, 2020).…”

Section: Discussionsupporting

confidence: 83%

Effect of Ethanol Vapor Treatment on the Growth of Alternaria alternata and Botrytis cinerea and Defense-Related Enzymes of Fungi-Inoculated Blueberry During Storage

Liu

et al. 2021

Front. Microbiol.

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The aim of the present study was to investigate the effects of ethanol vapor on the inhibition of Alternaria alternata and Botrytis cinerea in postharvest blueberry and the induction of defense-related enzymes (DREs) activities in fungi-inoculated blueberries stored at 0±0.5°C for 16days. Results indicated that ethanol vapor markedly inhibited the mycelial growth of A. alternata and B. cinerea in a dose-dependent manner, with inhibition rates of 9.1% (250μlL−1), 36.4% (500μlL−1), and 5.5% (1,000μlL−1) on A. alternata and 14.2% (250μlL−1), 44.7% (500μlL−1), and 76.6% (1,000μlL−1) on B. cinerea, respectively. Meanwhile, ethanol vapor also enhanced the activities of DREs in fungi-inoculated blueberries, including β-1,3-glucanase (GLU), chitinase (CHI), phenylalnine ammonialyase (PAL), peroxidase (POD), and polyphenol oxidase (PPO). In particular, 500μlL−1 ethanol vapor increased the activities of DREs by 84.7% (GLU), 88.0% (CHI), 37.9% (PAL), 85.5% (POD), and 247.0% (PPO) in A. alternata-inoculated blueberries and 103.8% (GLU), 271.1% (CHI), 41.1% (PAL), 148.3% (POD), and 74.4% (PPO) in B. cinerea-inoculated blueberries, respectively. But, the activity of PPO was decreased by 55.2 and 31.9% in 500μlL−1 ethanol-treated blueberries inoculated with A. alternata and B. cinerea, respectively, after 8days of storage. Moreover, the surface structure and ultrastructure of 500μlL−1 ethanol-treated blueberry fruit cells were more integrated than those of other treatments. The findings of the present study suggest that ethanol could be used as an activator of defense responses in blueberry against Alternaria and Botrytis rots, by activating DREs, having practical application value in the preservation of postharvest fruit and vegetables.

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Section: Discussionsupporting

confidence: 83%

Effect of Ethanol Vapor Treatment on the Growth of Alternaria alternata and Botrytis cinerea and Defense-Related Enzymes of Fungi-Inoculated Blueberry During Storage

Liu

et al. 2021

Front. Microbiol.

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“…Increased TAP in treated strawberry fruits may be the reason for the maintenance of ascorbic acid content, since phenolics have a protective effect [35], but this may also be attributed to a delay in the senescence process [26]. Similar effects of SA on total phenolic content have also been shown in table grape [42], cucumber [43], orange [35], pomegranate [25] apricot [40], and peach [44].…”

Section: Discussionmentioning

confidence: 91%

“…Thereby, these metabolites help to protect fruit from pathogens. This was also the case for terpinen-4-ol [42,43]. Increased TAP in treated strawberry fruits may be the reason for the maintenance of ascorbic acid content, since phenolics have a protective effect [35], but this may also be attributed to a delay in the senescence process [26].…”

Section: Discussionmentioning

confidence: 99%

Salicylate Treatment Affects Fruit Quality and Also Alters the Composition of Metabolites in Strawberries

et al. 2021

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This study evaluated the effect of preharvest treatments with salicylates (salicylic acid (SA), methyl salicylic acid (MeSA) and acetyl salicylic acid (ASA)) on fruit quality parameters and primary and secondary metabolites during ripening at five sampling dates. The results showed that salicylates affect overall fruit quality, and some very desirable and important properties of strawberry fruits were acquired by the treatments, such as a deeper red colour (decreased hue angle), delayed ripening process with maintenance of higher fruit firmness, and higher sugar and ascorbic acid content. HPLC-MS analysis of the phenolic contents showed at almost all sampling dates that treatment with salicylates increased the content of some phenolic groups, the contents of the hydroxycinnamic acids (SA: up to 18%; MeSA: up to 13% increase), flavanols (SA: up to 27%, MeSA: up to 36% and ASA: up to 24% increase), anthocyanins (SA: up to 51%, MeSA: up to 33% and ASA: up to 28% increase) and also flavonol glycosides such as flavones. Total phenolics in fruits also increased-up to 27% with SA and up to 28% with MeSA. In general, better fruit quality and higher metabolite content were obtained with SA.

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“…TTO is a potential anti-bacterial [ 7 , 17 ] and anti-fungal product [ 18 , 19 ], but there were few studies about the anti-MRSA activity of terpinen-4-ol, a main ingredient of TTO. Our research focused on the antibacterial activity of terpinen-4-ol against MRSA.…”

Section: Resultsmentioning

confidence: 99%

Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol

Cheng

Chen

et al. 2021

BMC Microbiol

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Background This study investigated the effects of terpinen-4-ol on methicillin-resistant Staphylococcus aureus (MRSA) and its biofilm, and the possible mechanisms governing this effect. Results We observed that terpinen-4-ol has good antibacterial activity and inhibits the formation of MRSA biofilm. The MIC and MBC values for terpinen-4-ol against S. aureus were 0.08% ~ 0.32%. And terpinen-4-ol at 0.32% could kill all bacteria and clear all biofilms. Untargeted metabolomic and transcriptomic analyses showed that terpinen-4-ol strongly inhibited DNA and RNA biosynthesis in MRSA at 2 h after treatment by affecting genes and metabolites related to purine and pyrimidine metabolic pathways. Some differential genes which play important roles in DNA synthesis and the production of eDNA from biofilm exposed to terpinen-4-ol was also significantly decreased compared with that of the control. Conclusions Terpinen-4-ol has good antibacterial activity and significantly inhibits the formation of MRSA biofilm by inhibiting purine and pyrimidine metabolism.

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Terpinen-4-ol Enhances Disease Resistance of Postharvest Strawberry Fruit More Effectively than Tea Tree Oil by Activating the Phenylpropanoid Metabolism Pathway

Cited by 51 publications

References 50 publications

Effect of Ethanol Vapor Treatment on the Growth of Alternaria alternata and Botrytis cinerea and Defense-Related Enzymes of Fungi-Inoculated Blueberry During Storage

Effect of Ethanol Vapor Treatment on the Growth of Alternaria alternata and Botrytis cinerea and Defense-Related Enzymes of Fungi-Inoculated Blueberry During Storage

Salicylate Treatment Affects Fruit Quality and Also Alters the Composition of Metabolites in Strawberries

Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol

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