Terpenoids enriched ethanol extracts of aerial roots of Ceriops decandra (Griff.) and Ceriops tagal (Perr.) promote diuresis in mice

Abstract

Introduction: Ceriops decandra (CD) and Ceriops tagal (CT) are two traditionally used mangrove plants widely distributed along the coastal areas of South Asia, Africa, South Pacific. In this study, we evaluated the diuretic potential of aerial roots of CD, CT and assessed the effectiveness of the plants' terpenoids enriched bioactive constituents against human carbonic anhydrase (hCA) enzyme through molecular docking. Materials and methods: Firstly, the acute toxicity of CD and CT was evaluated in mice. In vivo diuretic activity was then studied in mice and the volume of excreted urine was measured. The urine was further examined for pH, density and Na þ , K þ , Cl À concentrations. From this, the saluretic, natriuretic, kaliuretic and CAI (carbonic anhydrase inhibitory) activities were calculated. Finally, total terpenoid contents (TTC) of the plant extracts were quantified and the terpenoids previously reported from both CD and CT were docked against four hCA isoforms -hCAII, hCAIV, hCAXII and hCAXIV. Results: In the acute toxicity assessment, no sign of toxicity was found. In diuretic activity evaluation, both extracts displayed substantial increase in urine volume, with CD being at top. Concentrations of Na þ , K þ and Cl À were also upsurged at a high dose of treatment (500 mg/kg). Both extracts at 500 mg/kg dose demonstrated potent saluretic, natriuretic and CAI activity. The TTC of CD was significantly higher than CT. In molecular docking analysis, greater binding affinity against hCA isoforms was demonstrated by the terpenoids reported from CD. Conclusion: Aerial roots of both CD and CT possess substantial diuretic activity with an inhibitory effect on CA. Here, diuretic potential as well as the total terpenoid content of CD were much greater between the two.

Evaluation of Diuretic Property of Argemone mexicana along with Molecular Docking Study

“…Therefore, βamyrin could also play a role in diuretic activity. Our literature findings also revealed that terpenoid-enriched plant extracts promote diuresis, which supports this result 74 .…”

Ultrasonic-Assisted and Microwave-Assisted Extraction of Phenolics and Terpenoids from Abelmoschus sagittifolius (Kurz) Merr Roots Using Natural Deep Eutectic Solvents

“…TTC was quantified by the Biswajit Biswas method, and TPC was determined using the Folin–Ciocalteu reagent. , TPC and TTC were presented as milligrams of gallic acid equivalent per gram of dried weight (mg GAE/g dw) and milligrams of ursolic acid per gram of dried weight (mg UA/g dw), respectively.…”

Ultrasonic‐assisted and enzymatic‐assisted extraction to recover tannins, flavonoids, and terpenoids from used tea leaves using natural deep eutectic solvents

“…The method of determination of tannins content was prescribed by (Myo & Khat‐udomkiri, 2022). Terpenoid was measured referring to the method of determination of terpenoid in aerial roots of Ceriops decandra and Ceriops tagal (Biswas et al ., 2021). The results of TFC, TTAC, and TTC were expressed as milligram rutin, catechin, and ursolic acid equivalent per gram of dry basis (mg RE/g db, mg CE/g db, and mg UE/g db), respectively.…”