Terpene Glycosides from Sanguisorba officinalis and Their Anti-Inflammatory Effects

Guo, Da‐Le; Chen, Jinfeng; Tan, Lu; Jin, Meng-Ying; Ju, Feng; Cao, Zhixing; Deng, Fang; Wang, Lina; Gu, Yu‐Cheng; Deng, Yun

doi:10.3390/molecules24162906

Cited by 23 publications

(12 citation statements)

References 15 publications

(17 reference statements)

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“…Exposure to copper can cause injuries to superficial tissues, in particular to the hair cells of the zebrafish lateral line, followed by an acute inflammatory response [49,50]. Thus, treatment of zebrafish embryos/larvae with copper is today commonly used to analyze inflammatory response [65,66]. Since cyanobacterial EPS can bind dissolved heavy metal bipositive cations such as Cu 2+ or Pb 2+ [51], Phormidium EPS was applied to copper-treated larvae only after multiple washes.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

et al. 2020

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The Euganean Thermal District (Italy) represents the oldest and largest thermal center in Europe, and its therapeutic mud is considered a unique product whose beneficial effects have been documented since Ancient Roman times. Mud properties depend on the heat and electrolytes of the thermal water, as well as on the bioactive molecules produced by its biotic component, mainly represented by cyanobacteria. The investigation of the healing effects of compounds produced by the Euganean cyanobacteria represents an important goal for scientific validation of Euganean mud therapies and for the discovering of new health beneficial biomolecules. In this work, we evaluated the therapeutic potential of exopolysaccharides (EPS) produced by Phormidium sp. ETS05, the most abundant cyanobacterium of the Euganean mud. Specifically, Phormidium EPS resulted in exerting anti-inflammatory and pro-resolution activities in chemical and injury-induced zebrafish inflammation models as demonstrated using specific transgenic zebrafish lines and morphometric and expression analyses. Moreover, in vivo and in vitro tests showed no toxicity at all for the EPS concentrations tested. The results suggest that these EPS, with their combined anti-inflammatory and pro-resolution activities, could be one of the most important therapeutic molecules present in the Euganean mud and confirm the potential of these treatments for chronic inflammatory disease recovery.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

et al. 2020

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“…Furthermore, a neuropeptide, pituitary adenylate cyclase-activating polypeptide(PACAP)-38, known to be an anti-apoptotic and anti-inflammatory factor, was reported to inhibit neutrophil migration toward the neuromasts and expression of pro-inflammatory genes ( il8, il1b, il6 , and atf3 ) (Kasica-Jarosz et al, 2018 ). Several natural products were reported to exert an inhibitory effect on the CuSO 4 -induced neutrophil accumulation, including a new terpene glycoside extracted from the root of Sanguisorba officinalis (Guo et al, 2019 ), an enzymatic peptide (SEP) from skipjack ( Katsuwonus pelamis ) (Wang et al, 2019 ) and an extract from Quzhou Fructus Aurantii, an unripe fruit from the bitter orange tree ( Rutaceae Citrus changshan-huyou Y. B. Chang) (Li et al, 2018 ).…”

Section: Inflammatory Disease Models In Zebrafishmentioning

confidence: 99%

Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs

Xie

Meijer

Schaaf

2021

Front. Cell Dev. Biol.

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Dysregulation of the inflammatory response in humans can lead to various inflammatory diseases, like asthma and rheumatoid arthritis. The innate branch of the immune system, including macrophage and neutrophil functions, plays a critical role in all inflammatory diseases. This part of the immune system is well-conserved between humans and the zebrafish, which has emerged as a powerful animal model for inflammation, because it offers the possibility to image and study inflammatory responses in vivo at the early life stages. This review focuses on different inflammation models established in zebrafish, and how they are being used for the development of novel anti-inflammatory drugs. The most commonly used model is the tail fin amputation model, in which part of the tail fin of a zebrafish larva is clipped. This model has been used to study fundamental aspects of the inflammatory response, like the role of specific signaling pathways, the migration of leukocytes, and the interaction between different immune cells, and has also been used to screen libraries of natural compounds, approved drugs, and well-characterized pathway inhibitors. In other models the inflammation is induced by chemical treatment, such as lipopolysaccharide (LPS), leukotriene B4 (LTB4), and copper, and some chemical-induced models, such as treatment with trinitrobenzene sulfonic acid (TNBS), specifically model inflammation in the gastro-intestinal tract. Two mutant zebrafish lines, carrying a mutation in the hepatocyte growth factor activator inhibitor 1a gene (hai1a) and the cdp-diacylglycerolinositol 3-phosphatidyltransferase (cdipt) gene, show an inflammatory phenotype, and they provide interesting model systems for studying inflammation. These zebrafish inflammation models are often used to study the anti-inflammatory effects of glucocorticoids, to increase our understanding of the mechanism of action of this class of drugs and to develop novel glucocorticoid drugs. In this review, an overview is provided of the available inflammation models in zebrafish, and how they are used to unravel molecular mechanisms underlying the inflammatory response and to screen for novel anti-inflammatory drugs.

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“…1 Currently, S. officinalis has been widely used in traditional Chinese medicine for the treatment of inflammation, scalds, haemorrhage and leukopenia in patients. 2,3 Sanguisorba parviflora (Maxim) Takeda (Sp. T) belongs to Sanguisorba, which belongs to the Rosaceae family and is abundant in the Heilongjiang, Jilin and Liaoning Province.…”

Section: What Is K Nown and Objec Tive Smentioning

confidence: 99%

Sanguisorba parviflora (Maxim) Takeda alleviates cyclophosphamide‐induced leukopenia via regulating the hematopoietic cell‑specific protein 1‑associated protein X‑1 gene

Meng

et al. 2021

J Clin Pharm Ther

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What is known and the objective: Our previous studies have shown that saponins of Sanguisorba parviflora (Maxim) Takeda (Sp. T) relieved cyclophosphamide-induced myelosuppression in mice with leukopenia. The hematopoietic cell-specific protein 1-associated protein X-1 (HAX-1) participated in the survival of neutrophils through the regulation of mitochondrial function. This study aimed to comprehensively identify the role of HAX-1 in Sp. T to alleviate leukopenia.Methods: HAX-1 expression was examined in the peripheral blood neutrophils using real-time polymerase chain reaction (PCR), Western blot analysis and immunohistochemical staining. Neutrophil apoptosis was measured by flow cytometry. Mitochondrial function was evaluated via reactive oxygen species (ROS) generationand mitochondrial membrane potential (ΔΨm) integrity.Results and Discussion: Our study indicated that the expression of the HAX-1 gene was significantly decreased in the peripheral blood neutrophils of leukopenia patients compared with healthy donors. The saponins of Sp. T induced HAX-1 expression and promoted myeloid progenitor cell (mEB8-ER cell) viability, while overexpression of HAX-1 reduced the production of reactive oxygen species (ROS) and maintained the integrity of the mitochondrial membrane potential. Cyclophosphamide-induced mitochondrial dysfunction and apoptosis could be abrogated by treatment with Sp. T or the addition of metformin. What is new and our Conclusion:Our data support a mechanism where Sp. T protects against chemotherapy-induced leukopenia by regulating HAX-1 gene expression in a mitochondrial-dependent manner. K E Y W O R D Sapoptosis, leukopenia, mitochondrial function, Sanguisorba parviflora (Maxim) Takeda (Sp. T), the haematopoietic cell specific protein 1 associated protein X 1 (HAX1) | 1335 MENG Et al.

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Terpene Glycosides from Sanguisorba officinalis and Their Anti-Inflammatory Effects

Cited by 23 publications

References 15 publications

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs

Sanguisorba parviflora (Maxim) Takeda alleviates cyclophosphamide‐induced leukopenia via regulating the hematopoietic cell‑specific protein 1‑associated protein X‑1 gene

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