Terpene Bioconversion – How does its Future Look?

Krings, Ulrich; Berger, Ralf G.

doi:10.1177/1934578x1000500927

Cited by 20 publications

(26 citation statements)

References 122 publications

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“…This has also been important for testing our understanding for biochemical processes in general and understanding the genetic and biochemical components associated with terpene biosynthetic enzymes (Mandel et al 1996). Second, generating transgenic plants accumulating altered amounts of a specific terpene or suite of terpenes can be evaluated for their health-promoting properties (Sawai and Saito 2011) or could provide new means for the sustainable production of high-value chemicals for industrial uses (Krings and Berger 2010). …”

Section: Introductionmentioning

confidence: 99%

Engineering triterpene metabolism in tobacco

Jiang

Kempinski

et al. 2012

Planta

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Terpenes comprise a distinct class of natural products that serve a diverse range of physiological functions, provide for interactions between plants and their environment and represent a resource for many kinds of practical applications. To better appreciate the importance of terpenes to overall growth and development, and to create a production capacity for specific terpenes of industrial interest, we have pioneered the development of strategies for diverting carbon flow from the native terpene biosynthetic pathways operating in the cytosol and plastid compartments of tobacco for the generation of specific classes of terpenes. In the current work, we demonstrate how difficult it is to divert the 5-carbon intermediates DMAPP and IPP from the mevalonate pathway operating in the cytoplasm for triterpene biosynthesis, yet diversion of the same intermediates from the methylerythritol phosphate pathway operating in the plastid compartment leads to the accumulation of very high levels of the triterpene squalene. This was assessed by the co-expression of an avian farnesyl diphosphate synthase and yeast squalene synthase genes targeting metabolism in the cytoplasm or chloroplast. We also evaluated the possibility of directing this metabolism to the secretory trichomes of tobacco by comparing the effects of trichome-specific gene promoters to strong, constitutive viral promoters. Surprisingly, when transgene expression was directed to trichomes, high-level squalene accumulation was observed, but overall plant growth and physiology were reduced up to 80 % of the non-transgenic controls. Our results support the notion that the biosynthesis of a desired terpene can be dramatically improved by directing that metabolism to a non-native cellular compartment, thus avoiding regulatory mechanisms that might attenuate carbon flux within an engineered pathway.

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Section: Introductionmentioning

confidence: 99%

Engineering triterpene metabolism in tobacco

Jiang

Kempinski

et al. 2012

Planta

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“…Park et al [19] also found that LAB signi cantly reduced the terpene content of blueberry juice, including a 92% reduction in vitispirane. The causes of terpene reduction were not fully known, but could involve oxidation to secondary products, glycoside hydrolysis, or ester conversion, as well as isomerization and / or interconversion of some monoterpenols [13,[23][24][25].…”

Section: Discussionmentioning

confidence: 99%

“…Belviso et al [26] found that LAB cultures can completely degrade alpha-campholenal and form a new monoterpenoid in 48 hours. Although terpenes are formally composed of one biosynthetic unit, the fact that they can be biotransformed by mechanisms including hydration, isomerization, dehydrogenation, conjugation, oxidation, reduction, decarboxylation, and β-oxidation, means that multiple structures can be produced [2,13]. Microorganisms that promote the biological transformation of terpenes include bacteria, fungi, and yeast.…”

Section: Discussionmentioning

confidence: 99%

“…Microorganisms that promote the biological transformation of terpenes include bacteria, fungi, and yeast. These microorganisms can transform the original terpenes into new ones and other substances via various biotransformation reactions [2,13]. Thus, LAB is responsible for both the degradation of terpenes and the production of the new terpenoidic metabolites.…”

Section: Discussionmentioning

confidence: 99%

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Enriching the Nutritive Value of Marigold (Tagetes Erecta L) Crop Residues as a Ruminant Feed by Lactic Acid During Ensilage

Hou

Liu

Cai

et al. 2020

Preprint

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Background: Marigold (Tagetes erecta L) accounts for over half of the world's loose flower production, and marigold crop residues (MCR) are abundantly available and should be used as a forage. In this study, MCR from the last commercial flower pickings was ensilaged with lactic acid bacteria (LAB) and the shift in their volatile organic compounds (VOCs) profiles was monitored. Samples were collected at 6 different times during ensilage (3, 6, 9, 12, 15, 30 days) to determine and quantify the VOCs changes using a solid-phase microextraction (SPME) technique and gas chromatography – mass spectrometry (GC-MS). Results: After 30 days, the caryophyllene and piprocyone, which account for 14.67% and 12.17% of total VOCs, decreased by 32.86% and 9.62% respectively, alcohols increased from 2.8% to 8.13%, and the acetic acid content increased by 560%. Conclusion: The results reported in this study show that during ensilage, LAB influences type and levels of VOCs. These results have shed light on our understanding of how to improve marigold residue odor and to enhance terpene degradation by lactic acid bacteria fermentation.

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“…Pleurotus species offer a number of additional technical advantages, i.e. sequenced genome, short life cycle, easy cultivation in solid and liquid media, and first biotechnological applications have become known (Cohen et al, 2002;Krings and Berger, 2010). In Pleurotus species, the mating system is controlled by two unlinked mat loci, A and B (bifactorial).…”

Section: Introductionmentioning

confidence: 97%