Terminology of bioanalytical methods (IUPAC Recommendations 2018)

Labuda, Ján; Bowater, Richard P.; Fojta, Miroslav; Gauglitz, Günter; Glatz, Zdeněk; Hapala, Ivan; Havliš, Jan; Kilár, Ferenc; Kilár, Anikó; Malinovská, Lenka; Sirén, Heli M. M.; Skládal, Petr; Torta, Federico; Valachovič, Martin; Wimmerová, Michaela; Zdráhal, Zbyněk; Hibbert, David Brynn

doi:10.1515/pac-2016-1120

Cited by 22 publications

(10 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We also calculated the specific enzyme activity of human GABA-T, which is an important measure of enzyme purity. One enzyme unit (1 U = 1 µmol/min) is defined as the amount of the enzyme that catalyses the conversion of 1 µmol of the substrate into product per minute under the specified conditions of the assay method 42 . Human GABA-T at a concentration of 2.5 µg/mL converted 0.00125 µmol of the substrate to product per minute per mL (pH 8.6, 25 °C), which is defined as enzyme activity (0.00125 U/mL).…”

Section: Resultsmentioning

confidence: 99%

High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays

Park

Han

Kim

et al. 2021

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug design studies on GABA-T have been performed by using porcine GABA-T mostly and even bacterial GABA-T. Here we report an optimised protocol for overexpression of 6xHis-tagged human GABA-T in human cells followed by a two-step protein purification. Then, we established an optimised human GABA-T (0.5 U/mg) activity assay. Finally, we compared the difference between human and bacterial GABA-T in sensitivity to two irreversible GABA-T inhibitors, gabaculine and vigabatrin. Human GABA-T in homodimeric form showed 70-fold higher sensitivity to vigabatrin than bacterial GABA-T in multimeric form, indicating the importance of using human GABA-T. In summary, our newly developed protocol can be an important first step in developing more effective human GABA-T modulators.

show abstract

Section: Resultsmentioning

confidence: 99%

High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays

Park

Han

Kim

et al. 2021

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

show abstract

“…A dictionary containing search terms and screening. Freely available recommendations on the terminology of methods of bioanalytical chemistry from IUPAC [ 12 ] were used to create a dictionary. Terms were extracted from the aforementioned book [ 12 ] along with their categories: Enzymatic methods, Immunoanalytical methods, Genomics and Nucleic Acid Analysis, Proteomics, Metabolomics, Glycomics, and Lipidomics.…”

Section: Methodsmentioning

confidence: 99%

Identification of Potential Therapeutic Targets on the Level of DNA/mRNAs, Proteins and Metabolites: A Systematic Mapping Review of Scientific Texts’ Fragments from Open Targets

Pogodin

Kiseleva

Ilgisonis

2023

CIMB

View full text Add to dashboard Cite

Database records contain useful information, which is readily available, but, unfortunately, limited compared to the source (publications). Our study reviewed the text fragments supporting the association between the biological macromolecules and diseases from Open Targets to map them on the biological level of study (DNA/RNA, proteins, metabolites). We screened records using a dictionary containing terms related to the selected levels of study, reviewed 600 hits manually and used machine learning to classify 31,260 text fragments. Our results indicate that association studies between diseases and macromolecules conducted on the level of DNA and RNA prevail, followed by the studies on the level of proteins and metabolites. We conclude that there is a clear need to translate the knowledge from the DNA/RNA level to the evidence on the level of proteins and metabolites. Since genes and their transcripts rarely act in the cell by themselves, more direct evidence may be of greater value for basic and applied research.

show abstract

“…A biosensor is an analytical device that converts chemical/biochemical information into a useful analytical signal [ 54 , 55 , 56 ]. It is always composed of two basic elements: a bioreceptor, a selective and specific biological recognition element such as enzyme(s), DNA, antibodies among others; and a transducer, which converts the receptor-analyte interaction into an analytical (i.e., optical or electrical) signal whose intensity is directly or inversely proportional to the analyte concentration [ 57 ].…”

Section: Biosensor As An Alternative Device For Sepsismentioning

confidence: 99%

Emerging Biosensing Technologies towards Early Sepsis Diagnosis and Management

et al. 2022

View full text Add to dashboard Cite

Sepsis is defined as a systemic inflammatory dysfunction strictly associated with infectious diseases, which represents an important health issue whose incidence is continuously increasing worldwide. Nowadays, sepsis is considered as one of the main causes of death that mainly affects critically ill patients in clinical settings, with a higher prevalence in low-income countries. Currently, sepsis management still represents an important challenge, since the use of traditional techniques for the diagnosis does not provide a rapid response, which is crucial for an effective infection management. Biosensing systems represent a valid alternative due to their characteristics such as low cost, portability, low response time, ease of use and suitability for point of care/need applications. This review provides an overview of the infectious agents associated with the development of sepsis and the host biomarkers suitable for diagnosis and prognosis. Special focus is given to the new emerging biosensing technologies using electrochemical and optical transduction techniques for sepsis diagnosis and management.

show abstract

Terminology of bioanalytical methods (IUPAC Recommendations 2018)

Cited by 22 publications

References 87 publications

High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays

High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays

Identification of Potential Therapeutic Targets on the Level of DNA/mRNAs, Proteins and Metabolites: A Systematic Mapping Review of Scientific Texts’ Fragments from Open Targets

Emerging Biosensing Technologies towards Early Sepsis Diagnosis and Management

Contact Info

Product

Resources

About