Terminalia arjuna extract and arjunic acid mitigate cobalt chloride–induced hypoxia stress–mediated apoptosis in H9c2 cells

Manu, T. Mohan; Anand, T.; Pandareesh, M. D.; Kumar, P Bhuvanesh; Khanum, Farhath

doi:10.1007/s00210-019-01654-x

Cited by 14 publications

(8 citation statements)

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“…In a study investigating the protective effect of arjunic acid on cobalt chloride-induced hypoxic injury and apoptosis in rat cardiomyocytes, treatment of cells with 1.2 mM cobalt chloride for 24 h induced cytotoxicity [ 33 ]. In another study using ursodeoxycholic acid to protect cardiomyocytes from cobalt chloride-induced hypoxia, treatment with cobalt chloride for 24 h also induced cytotoxicity [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of action is the substitution of Co2+ with Fe2+ in prolyl hydroxylases (PHDs), key enzymes that correlate O2 concentration with HIF degradation under normal oxygen conditions [ 13 ]. The use of cobalt chloride to construct hypoxia models is widely used in scientific research, including the use of cobalt chloride to study the effects of hypoxia on respiratory plasticity and oxygen uptake in rat liver mitochondria [ 32 ], the study of cobalt chloride-induced hypoxic stress in rat myocardial H9c2 cells [ 33 ], and the study of the effect of the chemical inducer cobalt chloride and hypoxia on apoptosis in the human hepatocellular carcinoma cell line HepG2 [ 34 ]. Studies have pointed out that there is a relationship between FAM13A gene and HIF.…”

Section: Introductionmentioning

confidence: 99%

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FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

et al. 2021

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The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2′-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

et al. 2021

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“…The H9c2 cells which were cultured on the tissue culture plates were exposed to different concentrations (200, 400, 600, and 800 µM) of CoCl 2 (figure 7(a)) to find the effective dose of CoCl 2 . According to figure 7(a), MTT assay results exhibited that the addition of CoCl 2 within the culture media could significantly decrease the cell viability [97]. According to the obtained results, the CoCl 2 medium with 600 µM (IC 50 value) was selected for the following assessments.…”

Section: Hypoxia Simulationmentioning

confidence: 99%

“…Previous studies have shown that the presence of antioxidant agents can inhibit the damage, induced by CoCl 2 on the cells [97,98]. MTT assay was performed to analyze the cardioprotective properties of cardiac patches in hypoxia conditions (figure 7(b)).…”

Section: Hypoxia Simulationmentioning

confidence: 99%

Fabrication of a tailor-made conductive polyaniline/ascorbic acid-coated nanofibrous mat as a conductive and antioxidant cell-free cardiac patch

Moradikhah,

Shabani,

Tafazzoli Shadpour

2024

Biofabrication

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Polyaniline (PANI) was in-situ polymerized on nanofibrous polycaprolactone mats as cell-free antioxidant cardiac patches (CPs), providing electrical conductivity and antioxidant properties. The fabricated CPs took advantage of intrinsic and additive antioxidant properties in the presence of PANI backbone and ascorbic acid as a biocompatible dopant of PANI. The antioxidant nature of CPs may reduce the serious repercussions of oxidative stress, produced during the ischemia-reperfusion (I/R) process following myocardial infarction. The polymerization parameters were considered as Aniline (60 mM, 90 mM, and 120 mM), ascorbic acid concentrations ([aniline]:[ascorbic acid]=3:0, 3:0.5, 3:1, 3:3), and polymerization time (1h and 3h). Mainly, the more aniline concentrations and polymerization time, the less sheet resistance was obtained. 1,1 diphenyl-2-picrylhydrazyl (DPPH) assay confirmed the dual antioxidant properties of prepared samples. The advantage of the employed in-situ polymerization was confirmed by the de-doping/re-doping process. Non-desirable groups were excluded based on their electrical conductivity, antioxidant properties, and biocompatibility. The remained groups protected H9c2 cells against oxidative stress and hypoxia conditions. Selected CPs reduced the intracellular ROS content and mRNA level of caspase-3 while the bcl-2 mRNA level was improved. Also, the selected cardiac patch could attenuate the hypertrophic impact of hydrogen peroxide on H9c2 cells. The in vivo results of the skin flap model confirmed the CP potency to attenuate the harmful impact of I/R.

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“…Various stressors including cobalt chloride, hydrogen peroxide and Lipopolysaccharide are used to induce oxidative stress in vitro [27][28][29]. Monosodium iodoacetate (MIA) is usually used to induce stress in chondrocytes both in vitro and in vivo [30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Daphne mucronataenhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stressin vitro

et al. 2020

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Mesenchymal stem cells (MSCs) are being used to treat many diseases as they exhibit great regenerative potential. However, MSC's transplantation sometimes does not yield the maximum regenerative outcome as they are unable to survive in inflammatory conditions. Several approaches including preconditioning are used to improve the survival rate of mesenchymal stem cells. One such recently reported approach is preconditioning MSCs with plant extracts. The present study was designed to evaluate the effect of Daphne mucronata extract on stressed human adipose-derived mesenchymal stem cells (hADMSCs). Isolated hADMSCs were preconditioned with different concentrations of Daphne muconata extract and the protective, proliferative, antioxidant and anti-inflammatory effect was assessed through various assays and expression analysis of inflammatory markers regulated through NF-κB pathway. Results suggest that preconditioning hADMSCs with Daphne mucronata increased the cell viability, proliferative and protective potential of hADMSCs with a concomitant reduction in LDH, ROS and elevation in SOD activity. Moreover, both the ELISA and gene expression analysis demonstrated down regulations of inflammatory markers (IL1-β, TNF-α, p65, p50, MMP13) in Daphne mucronata preconditioned hADMSCs as compared to stress. This is the first study to report the use of MIA induced oxidative stress against hADMSC's and effect of Daphne mucronata on stressed hADMSCs. Results of these studies provided evidence that Daphne mucronata protects the hADMSCs during stress conditions by down regulating the inflammatory markers and hence increase the viability and proliferative potential of hADMSCs that is crucial for transplantation purposes.

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Terminalia arjuna extract and arjunic acid mitigate cobalt chloride–induced hypoxia stress–mediated apoptosis in H9c2 cells

Cited by 14 publications

References 50 publications

FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

Fabrication of a tailor-made conductive polyaniline/ascorbic acid-coated nanofibrous mat as a conductive and antioxidant cell-free cardiac patch

Daphne mucronataenhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stressin vitro

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