Terminal inverted repeats of insertion sequence IS30 serve as targets for transposition

Olasz, Ferenc; Farkas, Tibor; Kiss, János; Arini, Achille; Arber, Werner

doi:10.1128/jb.179.23.7551-7558.1997

Cited by 48 publications

(83 citation statements)

References 29 publications

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“…Tn3) or the extremities of other already integrated similar elements (e.g. IS21 or IS30) (Casadaban et al, 1981;Craig, 1997;Goryshin et al, 1998;Halling & Kleckner, 1982;Olasz et al, 1997;Reimmann & Haas, 1987;Sengstag et al, 1986;Tenzen & Ohtsubo, 1991;Zerbib et al, 1985). In S. agalactiae, it remains to be seen whether the transposition events described in this review affect all categories of genes whatever their function or whether they specifically affect sets of genes belonging to the same functional class.…”

Section: Discussionmentioning

confidence: 99%

Physiological impact of transposable elements encoding DDE transposases in the environmental adaptation of Streptococcus agalactiae

Fléchard

Gilot

2014

Microbiology

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We have referenced and described Streptococcus agalactiae transposable elements encoding DDE transposases. These elements belonged to nine families of insertion sequences (ISs) and to a family of conjugative transposons (TnGBSs). An overview of the physiological impact of the insertion of all these elements is provided. DDE-transposable elements affect S. agalactiae in a number of aspects of its capability to adapt to various environments and modulate the expression of several virulence genes, the scpB-lmB genomic region and the genes involved in capsule expression and haemolysin transport being the targets of several different mobile elements. The referenced mobile elements modify S. agalactiae behaviour by transferring new gene(s) to its genome, by modifying the expression of neighbouring genes at the integration site or by promoting genomic rearrangements. Transposition of some of these elements occurs in vivo, suggesting that by dynamically regulating some adaptation and/or virulence genes, they improve the ability of S. agalactiae to reach different niches within its host and ensure the 'success' of the infectious process.

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Section: Discussionmentioning

confidence: 99%

Physiological impact of transposable elements encoding DDE transposases in the environmental adaptation of Streptococcus agalactiae

Fléchard

Gilot

2014

Microbiology

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“…This product presumably arose by SSD or by transposition of the IRR-IRL junction to the IR end of the full IS copy, as shown for IS911 (Turlan et al, 2000) and IS30 (Kiss & Olasz, 1999;Olasz et al, 1997). The C5 dimers were similar to those that arose from C3 and the SR was identical with the sequence originally separating the truncated IRs in C4 (Fig.…”

Section: Detection Of Transposition Activities Characteristic For Is mentioning

confidence: 80%

“…This cleavage site in the cases of IS30 (Olasz et al, 1997(Olasz et al, , 1998 and IS911 (Ton-Hoang et al, 1998;Turlan et al, 2000) proved to be at the same distance from the end of IRs both in dimerization or integration into the target site, resulting in the same length of the spacer and of the target duplication. On the other hand, the spacer in (IS2) 2 (Szeverényi et al, 1996a) and in (IS21) 2 (Reimmann & Haas, 1987) does not correspond to the target duplication characteristic for these elements.…”

Section: Discussionmentioning

confidence: 98%

“…Such junctions have been identified in IS dimers consisting of two directly repeated elements and in minicircles formed by circularization of a single element. IS dimers have been isolated from IS2 (Szeverényi et al, 1996a), IS21 (Reimmann & Haas, 1987, 1990Reimmann et al, 1989), IS30 (Dalrymple, 1987;Olasz et al, 1993Olasz et al, , 1997, IS256 (Prudhomme et al, 2002) and IS911 (Turlan et al, 2000), and covalently closed circular IS elements have been described for IS1 (Sekine et al, 1997a, Turlan & Chandler, 1995, IS2 (Lewis & Grindley, 1997), IS3 (Sekine et al, 1994), IS30 (Kiss & Olasz, 1999), IS117 (Smokvina et al, 1994), IS150 (Welz, 1993;Haas & Rak, 2002), IS256 (Prudhomme et al, 2002) and IS911 (Polard et al, 1992(Polard et al, , 1996. For some of these elements both circular and dimeric forms have been reported, suggesting a possible connection between the formation and transposition of these structures, as described for IS30 (Kiss & Olasz, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements

et al. 2003

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This study demonstrates that Escherichia coli insertion elements IS3, IS150 and IS186 are able to form transpositionally active head-to-tail dimers which show similar structure and transpositional activity to the dimers of IS2, IS21 and IS30. These structures arise by joining of the left and right inverted repeats (IRs) of two elements. The resulting junction includes a spacer region (SR) of a few base pairs derived from the flanking sequence of one of the reacting IRs. Head-to-tail dimers of IS3, IS150 and IS186 are unstable due to their transpositional activity. They can be resolved in two ways that seem to form a general rule for those elements reported to form dimers. One way is a sitespecific process (dimer dissolution) which is accompanied by the loss of one IS copy along with the SR. The other is 'classical' transposition where the joined ends integrate into the target DNA. In intramolecular transposition this often gives rise to deletion formation, whereas in intermolecular transposition it gives rise to replicon fusion. The results presented for IS3, IS150 and IS186 are in accordance with the IS dimer model, which is in turn consistent with models based on covalently closed minicircles.

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“…The IR-IR joint bracketing a 2-bp spacer is composed of the 26-bp left and right IR ends (IRL and IRR, respectively) that differ at three positions (12). IR-IR joints occur in both IS dimers and minicircles that can integrate into hot spot sequences or next to other IS30 ends (13)(14)(15). Whereas transposition into a hot spot is a conventional way of translocation, which accompanies the resolution of IR-IR junction, targeting an IR end seems more specific for IS30 and generates a new IR-IR joint.…”

mentioning

confidence: 99%

Site-specific recombination by the DDE family member mobile element IS30transposase

Kiss

Szabó

Olasz

2003

Proc. Natl. Acad. Sci. U.S.A.

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DNA rearrangements carried out by site-specific recombinases and transposases (Tpases) show striking similarities despite the wide spectrum of the catalytic mechanisms involved in the reactions. Here, we show that the bacterial insertion sequence (IS)30 element can act similarly to site-specific systems. We have developed an inversion system using IS30 Tpase and a viable phage, where the integration͞excision system is replaced with IS30. Both models have been proved to operate analogously to their natural counterpart, confirming that a DDE family Tpase is able to fulfill the functions of site-specific recombinases. This work demonstrates that distinction between transposition and site-specific recombination becomes blurred, because both functions can be fulfilled by the same enzyme, and both types of rearrangements can be achieved by the same catalytic mechanisms.I t is now well documented that genetic information can be reshuffled by mobile elements that are usually distinguished as site-specific systems and transposons. Even though the output of their activity can be simplified to insertion, deletion, or inversion of DNA segments, transposition and site-specific recombination are generally regarded as distinct phenomena (1). Classification according to the protein-sequence motifs revealed that some transposases (Tpases) and site-specific recombinases (Recases) with entirely different functions fall within the same family (2-6). This finding supports that mechanistically dissimilar enzymes can perform similar biological functions and suggests that transposition and site-specific recombination may be closer to each other in some respect than was supposed earlier. Further support for this idea may emerge from insertion sequence (IS) elements that form an active junction composed of the inverted repeats (IRs) (7-10). This IR-IR junction shows similarities in its structure and function to the recombination sites of sitespecific systems.The Escherichia coli element, IS30, encoding a Tpase with the well conserved DDE signature (11), belongs to the increasing class of elements that transpose through an intermediate formed by abutted IRs (8). The IR-IR joint bracketing a 2-bp spacer is composed of the 26-bp left and right IR ends (IRL and IRR, respectively) that differ at three positions (12). IR-IR joints occur in both IS dimers and minicircles that can integrate into hot spot sequences or next to other IS30 ends (13-15). Whereas transposition into a hot spot is a conventional way of translocation, which accompanies the resolution of IR-IR junction, targeting an IR end seems more specific for IS30 and generates a new IR-IR joint. The IRs, together with the adjacent IS sequences, are important for the directionality of recombination, because, as always, IRL-IRR junction arises through dimerization or targeting an IR (8, 13, 16). The most frequent reaction has been observed when both donor and target DNA contain an IR-IR junction (13). In this case, the rearrangement is remarkably reversible and resembles site-specific ...

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Terminal inverted repeats of insertion sequence IS30 serve as targets for transposition

Cited by 48 publications

References 29 publications

Physiological impact of transposable elements encoding DDE transposases in the environmental adaptation of Streptococcus agalactiae

Physiological impact of transposable elements encoding DDE transposases in the environmental adaptation of Streptococcus agalactiae

Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements

Site-specific recombination by the DDE family member mobile element IS30transposase

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