Terminal-conjugated non-aggregated constraints of gold nanoparticles on lateral flow strips for mobile phone readouts of enrofloxacin

Tian, Run; Ji, Jinyu; Zhou, Yangyang; Du, Yumei; Bian, Xiaojun; Zhu, Fulin; Liu, Gang; Deng, Shengyuan; Wan, Ying; Yan, Juan

doi:10.1016/j.bios.2020.112218

Cited by 29 publications

(13 citation statements)

References 67 publications

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“…The aforementioned results indicated that the aggregation of IMBs after reaction with ENR was likely driven by the increased hydrophobicity on the IMB surface upon ENR binding. The increased hydrophobicity can be explained by (1) the exposure of hydrophobic regions after the antigen− ENR Detection Based on Turbidity Measurements. As mentioned, IMBs aggregated in the presence of ENR.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Enrofloxacin (ENR) is a major member of the fluoroquinolone family with a broad antimicrobial spectrum against both Gram-positive and Gram-negative bacteria. − It has been extensively used in poultry and livestock for the treatment and prevention of animal diseases. , However, the abuse or incorrect use of ENR often results in residues in the environment and food, which may lead to the increased occurrence of resistant bacteria . Consequently, many countries and organizations, including China and the European Union, have set the maximum residue limits (MRLs) for the sum of ENR and ciprofloxacin to 100–300 μg kg –1 in animal tissues (GB 31650-2019; Commission Regulation (EU) No.…”

Section: Introductionmentioning

confidence: 99%

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Monovalent Antigen-Induced Aggregation (MAA) Biosensors Using Immunomagnetic Beads in Both Sample Separation and Signal Generation for Label-Free Detection of Enrofloxacin

Shen

Jia

Liang

et al. 2022

ACS Appl. Mater. Interfaces

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Exploring new functions of nanomaterials can help facilitate the development of biosensors for the detection of antibiotics. Herein, a new detection modality based on monovalent antigen-induced aggregation (MAA) of immunomagnetic beads (IMBs) was proposed for rapid and label-free detection of enrofloxacin (ENR), which endowed IMBs with the abilities of both sample separation and signal generation. In the presence of ENR, the initially well-dispersed IMBs were aggregated and the degree of aggregation was in a concentration-dependent manner. After exploring the mechanism underlying IMB aggregation and investigating the key parameters affecting it, a label-free biosensing platform was developed for rapid and sensitive detection of ENR. Based on the significant differences in the magnetic separation speed and size between the aggregated and well-dispersed IMBs, two methods were proposed for quantitatively determining ENR, i.e., measuring the turbidity of the IMB supernatant after magnetic separation for a given time and visualizing and calculating the grayscale value of the aggregated IMBs trapped on the surface of a nitrocellulose membrane. A three-dimensional (3D)-printed syringe was designed and fabricated for automatic filtration of IMBs. This immunosensor allowed for sensitive detection of ENR in less than 15 min without any labels. It exhibited a satisfactory limit of detection of 0.79 ng mL–1 and showed the feasibility for ENR detection of spiked chicken meat with recovery rates ranging from 74.8 to 98.3%. The MAA immunosensor can act as a promising tool to detect trace levels of ENR and has the potential to be applied to complex food samples.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monovalent Antigen-Induced Aggregation (MAA) Biosensors Using Immunomagnetic Beads in Both Sample Separation and Signal Generation for Label-Free Detection of Enrofloxacin

Shen

Jia

Liang

et al. 2022

ACS Appl. Mater. Interfaces

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show abstract

“…After magnetic separation, the reacted solution without For cholesterol detection, the experimental procedures were conducted as follows. Typically, 10 μL of cholesterol oxidase (1 mg mL −1 ) and 10 μL of varying concentrations of cholesterol (5,10,20,50,100,200, 500, and 1000 μΜ) were mixed with 80 μL of phosphate-buffered saline (PBS, pH 7.4). Then, the mixed solution was incubated at 37 °C for 30 min.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Rapid advances in nanoscience and nanotechnology − enable the creation of nanomaterials with unique properties and superior features, which have been widely applied in many research areas, including biosensing, − biomedicine, − catalysis, − and energy storage. − A typical example among them is the nanozyme, which possesses intrinsic enzyme-mimicking characteristics as a new generation of artificial enzymes. Significant research efforts have been made to explore nanozymes because of the unparalleled features of easy preparation, low cost, and high stability.…”

Section: Introductionmentioning

confidence: 99%

One-Dimensional Synergistic Core–Shell Nanozymes with Superior Peroxidase-like Activity for Ultrasensitive Colorimetric Detection of Blood Cholesterol

Peng

Zhang

Zeng

et al. 2020

ACS Appl. Bio Mater.

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In this paper, a simple and green strategy was proposed to fabricate one-dimensional core−shell Fe 3 O 4 @C/Ni nanocomposites. The rationally designed hybrid nanostructures notably exhibited an extremely excellent peroxidase-mimicking property, arising from the synergetic effects of Fe 3 O 4 , carbon, and Ni nanoparticles, along with the hollow and hierarchically porous nanostructures. Based upon the outstanding peroxidase-like activity and cholesterol oxidase cascade reaction, a label-free, ultrasensitive, and highly selective colorimetric assay for cholesterol determination has been developed. Under the optimized conditions, the colorimetric biosensor demonstrated a linear response to cholesterol ranging from 5 to 200 μM, with a relatively low detection limit of 0.17 μM. More importantly, cholesterol determination as low as 5 μM could be directly distinguished with the naked eye. In addition, we successfully determined the total cholesterol content in human serum samples with satisfactory accuracy and good precision. The Fe 3 O 4 @C/Ni nanocatalystbased colorimetric biosensor provides great potential in point-of-care testing in disease diagnosis.

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“…3,4 Unfortunately, owing to the overuse or abuse of ENR, its residues in edible animal tissues and environment has brought a variety of adverse effects to humans, including chronic or acute poisoning, allergic reactions, antibacterial resistance, gastrointestinal flora imbalance, etc. 5,6 Thus, various countries have legally restrained the maximum residue limits (MRLs) for ENR in animal-derived foods. For instance, Japan has set up MRLs in all chicken tissues at 10 μg/kg, while the Food and Drug Administration of United States disabled the use of ENR in poultry entirely.…”

mentioning

confidence: 99%

Ultrasensitive Competitive Lateral Flow Immunoassay with Visual Semiquantitative Inspection and Flexible Quantification Capabilities

Huang

Shi

et al. 2022

Anal. Chem.

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Antibiotics abuse has caused various problems threatening human health and ecological environment. Monitoring antibiotics residual levels is of great significance, yet still challenging for quantitative point-of-need testing with high-sensitivity and visual capability. Here we developed a competitive lateral flow immunoassay (CLFIA) platform with flexible readout for enrofloxacin (ENR), a regularly added antibiotic. To overcome the limitation of low sensitivity of traditional colloidal gold-based CLFIA, the three-dimensionally assembled gold nanoparticles (AuNPs) within dendritic silica scaffold were fabricated as signal reporters. The assembly structure effectively retained the intrinsic absorption features of hydrophobic AuNPs and greatly enhanced the light extinction ability of a single label for signal amplification. The obtained CLFIA strips can not only achieve qualitative screening of ENR at a very low concentration by naked eye (cutoff value: 0.125 ng/mL), but also enable ultrasensitive quantification of ENR by an optical scanner (limit of detection: 0.00195 ng/mL) or a smartphone (limit of detection: 0.0078 ng/mL). Moreover, to elaborate the visual inspection degree of CLFIA against traditional yes/no interpretation, a novel multirange gradient CLFIA strip was prepared for visually semiquantitative identification of ENR with four concentration ranges. The novel CLFIA platform demonstrated sensitive, specific, and reliable determination of ENR with flexible signal readout and provides a potential and invigorating pathway to point-of-need immunoassay of antibiotics.

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Terminal-conjugated non-aggregated constraints of gold nanoparticles on lateral flow strips for mobile phone readouts of enrofloxacin

Cited by 29 publications

References 67 publications

Monovalent Antigen-Induced Aggregation (MAA) Biosensors Using Immunomagnetic Beads in Both Sample Separation and Signal Generation for Label-Free Detection of Enrofloxacin

Monovalent Antigen-Induced Aggregation (MAA) Biosensors Using Immunomagnetic Beads in Both Sample Separation and Signal Generation for Label-Free Detection of Enrofloxacin

One-Dimensional Synergistic Core–Shell Nanozymes with Superior Peroxidase-like Activity for Ultrasensitive Colorimetric Detection of Blood Cholesterol

Ultrasensitive Competitive Lateral Flow Immunoassay with Visual Semiquantitative Inspection and Flexible Quantification Capabilities

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