Terapia fotodinámica con azul de metileno en úlceras cutáneas infectadas con Pseudomonas aeruginosa y Fusarium spp.

Aspíroz, Carmen; Sevil, M.; Toyas, Carla; Gilaberte, Yolanda

doi:10.1016/j.ad.2016.11.020

Cited by 24 publications

(10 citation statements)

References 15 publications

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“…MB has several indications in medicine; it has been used to treat methemoglobinemia, malaria and urinary tract infections. During the last two decades, MB has attracted interest as a photosensitizer especially in cancer research [35,36,54]. Recently, MB has become increasingly important in antimicrobial studies [54,55].…”

Section: Resultsmentioning

confidence: 99%

“…In some clinical cases, an effective PDT using MB as a photosensitizer for skin ulcers could be demonstrated. This kind of therapy led to clinical and microbial cure with no significant adverse effects [54]. According to the data obtained from release studies and the antifungal activity tests, we decided to use only bolalipid aerogels loaded with MB for the aPDT experiment.…”

Section: Resultsmentioning

confidence: 99%

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The Use of Artificial Gel Forming Bolalipids as Novel Formulations in Antimicrobial and Antifungal Therapy

et al. 2019

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The alarming growth of multi-drug resistant bacteria has led to a quest for alternative antibacterial therapeutics. One strategy to circumvent the already existing resistance is the use of photodynamic therapy. Antimicrobial photodynamic therapy (aPDT) involves the use of non-toxic photosensitizers in combination with light and in situ oxygen to generate toxic radical species within the microbial environment which circumvents the resistance building mechanism of the bacteria. Hydrogels are used ubiquitously in the biological and pharmaceutical fields, e.g., for wound dressing material or as drug delivery systems. Hydrogels formed by water-insoluble low-molecular weight gelators may potentially provide the much-needed benefits for these applications. Bolalipids are a superior example of such gelators. In the present work, two artificial bolalipids were used, namely PC-C32-PC and Me2PE-C32-Me2PE, which self-assemble in water into long and flexible nanofibers leading to a gelation of the surrounding solvent. The aim of the study was to create stable hydrogel formulations of both bolalipids and to investigate their applicability as a novel material for drug delivery systems. Furthermore, methylene blue—a well-known photosensitizer—was incorporated into the hydrogels in order to investigate the aPDT for the treatment of skin and mucosal infections using a custom designed LED device.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The Use of Artificial Gel Forming Bolalipids as Novel Formulations in Antimicrobial and Antifungal Therapy

et al. 2019

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“…A-PDT seems to be an important alternative to antibiotics. A-PDT causes non-selective damage to different organelles in microbes and photodynamic-resistant bacterial strains have rarely been reported [17][18][19][20][21]. Due to the problem of antibiotic resistance, photodynamic therapy (PDT) is being developed as a novel antimicrobial treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Aspiroz et al presented a study with the use of methylene blue PDT in two cases of chronic lower limb ulcers in which fungal and bacterial superinfection complicated management. The authors revealed that PDT with methylene blue is a valid option for the management of superinfected chronic ulcers, reducing the use of antibiotics and the induction of resistance [ 20 ]. On the basis of results obtained in our study and the results of previous studies, it seems that A-PDT, due to its mechanism of action, low invasiveness, and lack of significant side effects, offers a potential alternative for the treatment leg ulcers.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic Therapy for the Treatment of Infected Leg Ulcers—A Pilot Study

et al. 2021

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Chronic and infected leg ulcers (LUs) are painful, debilitating, resistant to antibiotics, and immensely reduce a patient’s quality of life. The purpose of our study was to demonstrate the efficacy of photodynamic therapy (PDT) for the treatment of infected chronic LUs. Patients were randomized into two experimental groups: the first group received 5-aminolevulinic acid photodynamic therapy (ALA-PDT) (10 patients), and the second group of 10 patients received local octenidine dihydrochloride (Octenilin gel) exposed to a placebo light source with an inserted filter that mimiced red light. In the PDT group, we used 20% ALA topically applied for 4 hrs and irradiation from a Diomed laser source with a wavelength of 630 nm at a fluency of 80 J/cm2. ALA-PDT was performed 10 times during a 14-day hospitalization in 10 patients of both sexes aged 40–85 years with chronic leg ulcers. Treatments were carried out at 3-week intervals for 3–5 cycles. At 8-month follow-up with the PDT group, complete remission (CR) was obtained in four patients (40%), partial response (>50% reduction in ulcer diameter) in four patients (40%), and no response in two patients (20%) who additionally developed deterioration of the local condition with swelling, erythema, and inflammation. To assess the degree of pain during the trials, we used a numeric rating scale (NRS). From the preliminary results obtained, we concluded that PDT can be used to treat leg ulcers as a minimally invasive and effective method with no serious side effects, although further studies on a larger group of patients with LUs are warranted.

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“…It is based on photosensitizer molecules with the propriety of being activated by visible light and react with oxygen generating reactive oxygen species, toxic to target cells (27). aPDT based on Methylene Blue (MB, MB-aPDT), the principal photosensitizer of the phenothiazine family, is a low-cost and easy-to-use technique that has already shown its antimicrobial applications in periodontal disease, impetigo or the exacerbation of human atopic dermatitis and the treatment cutaneous mycoses, leishmaniasis, and infected wounds (27)(28)(29)(30)(31) used in animals (32) and skin S. aureus murine models (33)(34)(35).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Staphylococcus aureus Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both

et al. 2021

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Background: Antibiotic resistance and impaired wound healing are major concerns in S. aureus superficial skin infections, and new therapies are needed. Antimicrobial photodynamic therapy (aPDT) is a new therapeutic approach for infections, but it also improves healing in many wound models.Objective: To compare the antimicrobial activity and the effects on wound healing of aPDT based on Methylene Blue (MB-aPDT) with mupirocin treatment, either alone or in combination, in superficial skin wounds of S. aureus-infected mice. Additionally, to evaluate the clinical, microbiological, and cosmetic effects on wound healing.Materials and Methods: A superficial skin infection model of S. aureus was established in SKH-1 mice. Infected wounds were treated with MB-aPDT, MB-aPDT with a daily topical mupirocin or only with mupirocin. No treatment was carried out in control animals. Daily clinical and microbiological examinations were performed until complete clinical wound healing. Histopathological studies and statistical analysis were performed at the end of the study.Results: MB-aPDT treatment induced the best wound healing compared to mupirocin alone or to mupirocin plus MB-aPDT. Superficial contraction at 24 h and a greater reduction in size at 48 h, quicker detachment of the crust, less scaling, and absence of scars were observed. Histopathological studies correlated with clinical and gross findings. By contrast, mupirocin showed the highest logaritmic reduction of S. aureus.Conclusions: MB-aPDT and mupirocin treatments are effective in a murine superficial skin infection model of S. aureus. One session of MB-aPDT was the best option for clinical wound healing and cosmetic results. The addition of mupirocin to MB-aPDT treatment improved antimicrobial activity; however, it did not enhance wound healing. No synergistic antibacterial effects were detected.

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Terapia fotodinámica con azul de metileno en úlceras cutáneas infectadas con Pseudomonas aeruginosa y Fusarium spp.

Cited by 24 publications

References 15 publications

The Use of Artificial Gel Forming Bolalipids as Novel Formulations in Antimicrobial and Antifungal Therapy

The Use of Artificial Gel Forming Bolalipids as Novel Formulations in Antimicrobial and Antifungal Therapy

Photodynamic Therapy for the Treatment of Infected Leg Ulcers—A Pilot Study

Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Staphylococcus aureus Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both

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