Terapia celular em gatos portadores de doença renal crônica: avaliação laboratorial e imagiológica

Abstract

Chronic kidney disease (CKD) is one of the most commom causes of illness and death in geriatric cats and leads to a loss of kidney function. The chronic form is characterized by persisting an extended period of time and it has a poor prognosis.Currently, fluid replacement, hemodialysis and kidney transplantation are the treatment options. As the stem cell therapy has been extensively studied in recent years due to its ability to improve the function of organ injured, including the kidneys, this study aimed to evaluate the effect of transplantation of stem cell derived from adipose tissue of cats as also study the contribution of laboratory exams and imaging in therapeutic evolution. Cells from adipose tissue showed fibroblastoid morphology, adherence to plastic; differentiated into osteocytes, adipocytes and chondrocytes and expressed surface markers characteristic of mesenchymal stem cells. Furthermore, when injected into immunocompromised nude mice showed no tumor formation. To screen animals with chronic kidney disease for this study were performed blood tests, ultrasound and urine of 97 animals, these seven animals had chosen profile and were included. In this study the animals were divided into three groups: placebo (A); cell therapy (B) and cell therapy associated with hydration (C). The results show that no significant difference between the groups, but this is due to our sample size.However two cats with CKD of group B experienced modest decrease in serum creatinine. Regarding serum cytokine expression profile, we found a significant increase of IL-6 in the group A; the IL10 behaved differently among the groups, with a slight increase in group B while group A presented significant reduction and TNFα and did not change over time. Thus, the intravenous injection of stem cells from adipose tissue may prevent the progression of disease in a subtle way.

"Mesenchymal Stem Cell Injection into Kidney Acupoints: A Case Report of A Feline With Chronic Kidney Disease"

“…MSCs are adherent, fibroblast-like cells that are able to differentiate into diverse cellular lineages and that, in certain microenvironments, can be induced to differentiate into specific lineages [5]. MSCs have been reported to have therapeutic potential in the treatment of kidney disease, especially due to their abilities to promote reno-protective effects, induce proliferation, promote angiogenesis at the affected location, inhibit the inflammatory response, and improve renal function [6,7].…”

>b<Isolamento, expansão e caracterização de células progenitoras renais derivadas de mesonefro e metanefro de gato doméstico (>i<Felis catus>/i<)>/b<

“…Diversos estudos vêm sendo realizados com foco na caracterização de células progenitoras renais, ou derivadas de outros órgãos e tecidos, no sentido de observar sua viabilidade e posterior aplicação em terapias celulares experimentais. Em gatos, já foram objeto de estudo por exemplo, as células-tronco mesenquimais derivadas da medula óssea e do âmnio (MARTIN et al, 2002;VIDANE et al, 2016), as células renais e cerebrais (JIMENEZ et al, 2001) e as células derivadas do tecido adiposo (SANTOS, 2012;.…”

“…Em gatos, inúmeras células já foram estudadas, tais como as células-tronco mesenquimais provenientes da medula óssea e do tecido adiposo. Estas células apresentam um alto potencial proliferativo em cultura, alta capacidade de diferenciação nos diversos tipos celulares existentes, além de um excelente potencial terapêutico(MARTIN et al, 2002;SANTOS, 2012;. doméstico apresentam efeitos paliativos, fornecendo um melhor bem-estar aos animais, porém não foram observados progressos significativos no tratamento para as DRC.Todos os estudos citados acima vêm nos fornecer subsídios para que continuamos na pesquisa com as terapias celulares, até o presente momento não foi possível obter um tratamento eficaz para essas doenças, porém esses estudos são bases para continuarmos pesquisando melhores modelos ou novas fontes alternativas de células para encontrarmos melhores prognósticos a essa enfermidade.…”