Terahertz signatures of biological-warfare-agent simulants

Globus, Tatiana; Woolard, Dwight L.; Khromova, T.; Partasarathy, Ramakrishnan; Majewski, Alexander; Abreu, Rene; Hesler, Jeffrey L.; Pan, S. K.; Ediss, G. A.

doi:10.1117/12.549128

Cited by 7 publications

(4 citation statements)

References 6 publications

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“…Several challenges however arise when trying to characterize an entire microorganis m. In the past, transmission spectra of bio-particles (cells and spores) have been investigated in the sub-THz range using photomixing [23][24][25] and FTIR technologies [12,[26][27][28][29][30][31] with a relatively good spectral resolution, better than 0.25 cm-1 Spectra reveal detectable resonant features in the range below 25 cm-1. To enhance the sensitivity, we used a Fourier spectrometer model FTS-66v with a Si-bolo meter operating at 1.7 K. Nevertheless, the intensity of spectral features in most cases was only 1-2 % for solid samp les, and the reproducibility was not good.…”

Section: Introductionmentioning

confidence: 99%

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Globus¹,

Dorofeeva²,

Sizov³

et al. 2012

AJBE

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In this work, sub-terahertz (THz) spectroscopy is applied to characterize lyophilized and in vitro cultured bacterial cells of non-pathogenic species of Escherichia coli (E. coli) and Bacillus subtilis (BG), spores of BG and DNA fro m E. coli. One of the goals of this research is to demonstrate that Fourier Transform (FT) spectroscopy in the frequency region of 10-25 cm -1 is sensitive enough to reveal characteristic spectral features fro m bio-cells and spores in different environment, to verify the d ifferences between species, and to show the response of spores to vacuum and response of cultured cells to heat. The experimental technique was significantly improved for sensitivity and reliab ility. Observed spectra taken with a spectral resolution of 0.25 cm -1 using FT spectrometer with a detector operating at 1.7 K are rich in well resolved features having spectral widths of ~0.5-1 cm -1 . The reproducibility of experimental results was verified and confirmed. Measured spectra from E. coli DNA and from the entire cell have many similarit ies, thus demonstrating that the cellu lar co mponents might contribute to the v ibrational spectrum of the cell. The results of this work confirm that observed spectroscopic features are caused by fundamental physical mechanism of interaction between THz rad iation and biological macro-mo lecules. Particu larly, the analysis of results indicates that the spectroscopic signatures of microorganis ms originate fro m the co mbination of low frequency vibrational modes or group of modes at close frequencies (vibrational bands) within mo lecular co mponents of bacterial cells/spores, with the significant contribution from the DNA. The significance of this study is justified by necessity for a fast and effective, label free and reagent free optical technology to protect against environmental and other biological threats, as well as for general medical research. The obtained results show that THz v ibrational spectroscopy promises to add quantitative genetic information to the characteristic signatures of biological objects, increasing the detection accuracy and selectivity when appropriate spectral resolution is used.

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Section: Introductionmentioning

confidence: 99%

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Globus¹,

Dorofeeva²,

Sizov³

et al. 2012

AJBE

Self Cite

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“…THz spectroscopy was assessed as an effective technique for remote detection of biological warfare agents [9]. For toxin Ovalbumin, bacteria Erwinia herbicola, Bacillus Subtilis, lyophilized cells, RNA MS2 phage, and BioGene, signatures were found within 3-10 cm -1 [10]. Transmission spectra of Ovalbumin were also measured in 15-680 cm -1 [11], see Fig.…”

Section: Results Of the Phase I Workmentioning

confidence: 99%

“…We will choose toxin Ovalbumin, Bacillus Subtilis, RNA from MS2 phage, and E. coli to test our spectrometer in the range of 5-33.3 cm -1 . We will compare our measurements of the spectra with those made by using FTIR [7][8][9][10][11][12][13][14]. Our goal is to demonstrate the advantages of our novel spectrometer in terms of sensitivity, spectral resolution, linewidith, tuning range, scanning speed, capability of sensing in the reflection mode, dynamic range, and false alarm rate due to artifact.…”

Section: According Tomentioning

confidence: 99%

Widely-tunable, Compact, and Portable Terahertz Source Based on Intracavity Difference-Frequency Generation in Dual-Frequency Yb:YAG Laser for Identification and Detection of Biological Agents

Zotova¹

2011

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“…The research results show that a variety of substances can be effectively identified. Globus et al (2004) focused on the identification and analysis of a variety of bacteria. Lu et al (2005) realized the detection of molecules based on THz spectrum.…”

Section: Terahertz For Quality Detectionmentioning

confidence: 99%

Dendrobium candidum quality detection in both food and medicine agricultural product: Policy, status, and prospective

Chen

Sun

et al. 2022

Front. Sustain. Food Syst.

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Dendrobium candidum (DC) is an agricultural product for both food and medicine. It has a variety of beneficial effects on the human body with antioxidant, anti-inflammatory, antitumor, enhancing immune function, and other pharmacological activities. Due to less natural distribution, harsh growth conditions, slow growth, low reproduction rate, and excessive logging, wild DC has been seriously damaged and listed as an endangered herbal medicine variety in China. At present, the quality of DC was uneven in the market, so it is very necessary to detect its quality. This article summarized the methods of DC quality detection with traditional and rapid nondestructive, and it also expounded the correlation between DC quality factor and endophytes, which provides a theoretical basis for a variety of rapid detection methods in macromolecules. At last, this article put forward a variety of rapid nondestructive detection methods based on the emission spectrum. In view of the complexity of molecular structure, the quality correlation established by spectral analysis was greatly affected by varieties and environment. We discussed the possibility of DC quality detection based on the molecular dynamic calculation and simulation mechanism. Also, a multimodal fusion method was proposed to detect the quality. The literature review suggests that it is very necessary to understand the structure performance relationship, kinetic properties, and reaction characteristics of chemical substances at the molecular level by means of molecular chemical calculation and simulation, to detect a certain substance more accurately. At the same time, several modes are combined to form complementarity, eliminate ambiguity, and uncertainty and fuse the information of multiple modes to obtain more accurate judgment results.

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Terahertz signatures of biological-warfare-agent simulants

Cited by 7 publications

References 6 publications

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Widely-tunable, Compact, and Portable Terahertz Source Based on Intracavity Difference-Frequency Generation in Dual-Frequency Yb:YAG Laser for Identification and Detection of Biological Agents

Dendrobium candidum quality detection in both food and medicine agricultural product: Policy, status, and prospective

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