Terahertz signature characterization of bio-simulants

Majewski, Alexander; Miller, Peter J.; Abreu, Rene; Grotts, Jeffrey; Globus, Tatiana; Brown, E. R.

doi:10.1117/12.603658

Cited by 5 publications

(5 citation statements)

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“…Several challenges however arise when trying to characterize an entire microorganis m. In the past, transmission spectra of bio-particles (cells and spores) have been investigated in the sub-THz range using photomixing [23][24][25] and FTIR technologies [12,[26][27][28][29][30][31] with a relatively good spectral resolution, better than 0.25 cm-1 Spectra reveal detectable resonant features in the range below 25 cm-1. To enhance the sensitivity, we used a Fourier spectrometer model FTS-66v with a Si-bolo meter operating at 1.7 K. Nevertheless, the intensity of spectral features in most cases was only 1-2 % for solid samp les, and the reproducibility was not good.…”

Section: Introductionmentioning

confidence: 99%

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Globus¹,

Dorofeeva²,

Sizov³

et al. 2012

AJBE

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In this work, sub-terahertz (THz) spectroscopy is applied to characterize lyophilized and in vitro cultured bacterial cells of non-pathogenic species of Escherichia coli (E. coli) and Bacillus subtilis (BG), spores of BG and DNA fro m E. coli. One of the goals of this research is to demonstrate that Fourier Transform (FT) spectroscopy in the frequency region of 10-25 cm -1 is sensitive enough to reveal characteristic spectral features fro m bio-cells and spores in different environment, to verify the d ifferences between species, and to show the response of spores to vacuum and response of cultured cells to heat. The experimental technique was significantly improved for sensitivity and reliab ility. Observed spectra taken with a spectral resolution of 0.25 cm -1 using FT spectrometer with a detector operating at 1.7 K are rich in well resolved features having spectral widths of ~0.5-1 cm -1 . The reproducibility of experimental results was verified and confirmed. Measured spectra from E. coli DNA and from the entire cell have many similarit ies, thus demonstrating that the cellu lar co mponents might contribute to the v ibrational spectrum of the cell. The results of this work confirm that observed spectroscopic features are caused by fundamental physical mechanism of interaction between THz rad iation and biological macro-mo lecules. Particu larly, the analysis of results indicates that the spectroscopic signatures of microorganis ms originate fro m the co mbination of low frequency vibrational modes or group of modes at close frequencies (vibrational bands) within mo lecular co mponents of bacterial cells/spores, with the significant contribution from the DNA. The significance of this study is justified by necessity for a fast and effective, label free and reagent free optical technology to protect against environmental and other biological threats, as well as for general medical research. The obtained results show that THz v ibrational spectroscopy promises to add quantitative genetic information to the characteristic signatures of biological objects, increasing the detection accuracy and selectivity when appropriate spectral resolution is used.

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Section: Introductionmentioning

confidence: 99%

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Globus¹,

Dorofeeva²,

Sizov³

et al. 2012

AJBE

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“…The Brown group is well known for its extensive research on the THz signatures of bacterial spores. A summary of the measured samples and corresponding candidate signatures is provided in Table 2. In the past decade, growing evidence has indicated that spectral signatures can be influenced by three main factors, with sample preparation (sample conformation) being first and foremost (Majewski et al 2005). More specifically, the dilute B. subtilis powder mixed with polyethylene powder displays stronger absorption depths and narrower signatures than the concentrated samples without polyethylene powder, which is attributed to the smearing of single-bioparticle electromagnetic resonances (Brown et al 2004).…”

Section: Bacterial Spore Identification By Thz Spectroscopymentioning

confidence: 98%

“…Based on mathematically sound principles of detection and estimation theory, signal processing methods can extract the signatures from clutter, interferants, and noise (Majewski et al 2005). As such, detection filters can be developed for bacterial spores using optimal algorithms after the collection of signature data.…”

Section: Bacterial Spore Identification By Thz Spectroscopymentioning

confidence: 99%

Terahertz spectroscopy for bacterial detection: opportunities and challenges

Xiang

Yang

Luo

et al. 2016

Appl Microbiol Biotechnol

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The demand for advanced bacterial detection tools is continuously increasing, promoted by its significant benefits in various applications. For instance, in the medical field, these tools would facilitate decision making about more tailored therapies once the infection source has been identified. In the past few years, terahertz (THz = 10(12) Hz) spectroscopy has also shown potential as a novel bacterial detection modality due to its unique advantages. Impressive breakthroughs have been achieved in this field related to bacterial component characterization, spore identification, and cell detection. However, some intrinsic limitations and technical bottlenecks have led to some debates about the practicability of its clinical adoption. In this review, we summarize the progress achieved in this field and discuss some challenges and strategies for future implementation of practical applications.

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“…For E. coli, transition peaks were identified in 10-25 cm -1 [12,13]. Based on measurements made on three bio-simulants [14], transition signatures existed in 120-750 GHz (4-25 cm -1 ). It was demonstrated that Bacillus Subtilis and spores in polyethylene and dilute water solutions still exhibited resonance peaks in the THz region [15,16].…”

Section: According Tomentioning

confidence: 99%

“…We will choose toxin Ovalbumin, Bacillus Subtilis, RNA from MS2 phage, and E. coli to test our spectrometer in the range of 5-33.3 cm -1 . We will compare our measurements of the spectra with those made by using FTIR [7][8][9][10][11][12][13][14]. Our goal is to demonstrate the advantages of our novel spectrometer in terms of sensitivity, spectral resolution, linewidith, tuning range, scanning speed, capability of sensing in the reflection mode, dynamic range, and false alarm rate due to artifact.…”

Section: According Tomentioning

confidence: 99%

Widely-tunable, Compact, and Portable Terahertz Source Based on Intracavity Difference-Frequency Generation in Dual-Frequency Yb:YAG Laser for Identification and Detection of Biological Agents

Zotova¹

2011

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Terahertz signature characterization of bio-simulants

Cited by 5 publications

References 0 publications

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Sub-THz Vibrational Spectroscopy of Bacterial Cells and Molecular Components

Terahertz spectroscopy for bacterial detection: opportunities and challenges

Widely-tunable, Compact, and Portable Terahertz Source Based on Intracavity Difference-Frequency Generation in Dual-Frequency Yb:YAG Laser for Identification and Detection of Biological Agents

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