Tep22, a novel testicular expressed gene, is involved in the biogenesis of the acrosome and the midpiece of the sperm tail

Neesen, Jürgen; Hartwich, Tobias M.P.; Brandhorst, Gunnar; Aumüller, Gerhard; Gläser, Birgitta; Burfeind, Peter; Mendoza-Lujambio, Irene

doi:10.1016/s0006-291x(02)02265-9

Cited by 11 publications

(11 citation statements)

References 41 publications

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“…Our Western blot analysis newly revealed that the Mm.23377 protein made as a 22 kDa-protein in testicular cells is changed to a higher molecular weight form between the stages of testicular sperm and mature sperm, suggesting post-translational modification. Consistent with this, the protein contains several putative glycosylation and phosphorylation sites [22]. Mm.159795, identified as CatSper3 [21], was found localized to the endoplasmic reticulum in GC-2 cells in this study.…”

Section: Discussionsupporting

confidence: 83%

“…Nonetheless, we did not eliminate them because we have obtained new information about these proteins in this investigation. Mm.23377, named Tep22, has been suggested to be involved in the biogenesis of the acrosome and the midpiece of the sperm tail [22]. Our Western blot analysis newly revealed that the Mm.23377 protein made as a 22 kDa-protein in testicular cells is changed to a higher molecular weight form between the stages of testicular sperm and mature sperm, suggesting post-translational modification.…”

Section: Discussionmentioning

confidence: 73%

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Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

Choi

Lee

et al. 2007

BMC Genomics

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 73%

Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

Choi

Lee

et al. 2007

BMC Genomics

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“…The presence of strong immunoreactivity in the Golgi/acrosomal region, coinciding with the onset of acrosomal biogenesis, is consistent with the earlier report on the association of this protein with the head region of a few mammalian spermatozoa (Bhat et al 1995). Such a pattern of immunolocalization that we observed is shared by several other acrosomal-specific proteins, such as Tep22 (Neesen et al 2002), proacrosin (Phi- Van et al 1983), protein disulfide isomerase (Ohtani et al 1993), SP-10 (Kurth et al 1993), SIAA (Jimenez et al 1994), rat 2B1 (Jones et al 1996), ACIII (Gautier-Courteille et al 1998), Kir3.2d (Inanobe et al 1999), Acrin1 (MN7) (OhOka et al 2001) and CYP51 (Cotman et al 2001). However, the site of biosynthesis of immunoreactive RCP in various mammalian species (Subramanian & Adiga 1996) remains to be investigated.…”

Section: Discussionsupporting

confidence: 71%

Germ cell-specific localization of immunoreactive riboflavin carrier protein in the male golden hamster: appearance during spermatogenesis and role in sperm function

Sreekumar¹,

Acharya²,

Lalitha³

et al. 2005

Reproduction

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Riboflavin carrier protein (RCP) is a phosphoglycoprotein (37 kDa) that is well studied in chicken. An immunologically crossreacting protein was identified in mammals and active immunization of male rats and bonnet monkeys with chicken RCP lead to an ,80% reduction in fertility. However, the physiological mechanism responsible for inhibition of male fertility has not been investigated. Moreover, information on the cell type-specific localization and the origin of immunoreactive RCP during spermatogenesis is extremely limited. Hence, studies were carried out to determine the pattern of expression of immunoreactive RCP during spermatogenesis and its role in sperm function in the golden hamster. Immunoreactive RCP was germ cell-specific, found to be associated with the acrosome-organizing region of early spermatids and showed interesting patterns of immunolocalization during late stages of spermiogenesis. Mature spermatozoa exhibited acrosome-specific localization, mainly in the peri-acrosomal membrane. The immunoreactive protein was undetectable in (non)gonadal somatic cells tested. The protein had a molecular mass of 45 -55 kDa and was biosynthesized by round spermatids. The acrosome-specific localization of immunoreactive RCP was unchanged during capacitation, but it was substantially lost during acrosome reaction. Functional studies indicated that treatment of spermatozoa with anti-RCP antibodies did not have any effect on either capacitation or acrosome reaction, but markedly reduced the rate of sperm penetration into zona-free hamster oocytes. These results show the existence of male germ cell-specific immunoreactive RCP, having a potential role in sperm -egg interaction in hamsters. Also the pattern of immunoreactive-RCP localization makes it an ideal marker to monitor development of acrosome in mammalian spermatozoa.

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“…Immunoelectron microscopic analyses were essentially performed as described (Neesen et al ., 2002). Briefly, mouse testes samples of TPC1 wild-type and TPC1-knockout animals were immersion fixed for 2 h in a fixative consisting of 2% paraformaldehyde and 0.05% glutaraldehyde in 0.05 M sodium cacodylate buffer (0.1 M, pH 7.3).…”

Section: Methodsmentioning

confidence: 99%

NAADP and the two-pore channel protein 1 participate in the acrosome reaction in mammalian spermatozoa

Arndt

Castonguay

Arlt

et al. 2014

MBoC

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Tep22, a novel testicular expressed gene, is involved in the biogenesis of the acrosome and the midpiece of the sperm tail

Cited by 11 publications

References 41 publications

Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

Germ cell-specific localization of immunoreactive riboflavin carrier protein in the male golden hamster: appearance during spermatogenesis and role in sperm function

NAADP and the two-pore channel protein 1 participate in the acrosome reaction in mammalian spermatozoa

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