Teor de cafeína em sementes matrizes do guaranazeiro

Spoladore, Dayse Soave; Boaventura, Marco Antonio Milan; Saes, Luis Alberto

doi:10.1590/s0006-87051987000200022

Cited by 11 publications

(5 citation statements)

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“…Caffeine concentration varies in the tissues of these plants as well as throughout their organs. Guarana (Paullinia cupana) seeds may contain up to 4% caffeine (Spoladore et al, 1987), while seeds of some coffee species are almost alkaloid-free (Cliffford et al, 1991). Caffeine has also been found in citrus flowers (Stewart, 1985).…”

Section: Composition Of the Coffee Seedmentioning

confidence: 99%

Evaluation of Endogenous Sugars, Chlorogenic Acid and Caffeine Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)

Isutsa

Mayoli

Nyende

et al. 2018

JAA

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Coffee is one of the most important cash crops cultivated in the world with great economic importance. During the induction of somatic embryogenesis, there are different endogenous compounds involved in the success or failure of the somatic embryogenesis response and these compounds determine the specificity of cellular responses. This present experiment identified and quantified endogenous sugars, chlorogenic acid and caffeine present during somatic embryogenesis of ‘Ruiru 11’. Laboratory experiments were set up at Coffee Research Institute, Ruiru-Kenya between 2014 and 2016. Third leaf pair explants were excised from 8-monthold greenhouse-grown mother plants and cultured in half strength Murashige and Skoog basal salts augmented with Thidiazuron. Once embryos had developed, the cultures were analysed for endogenous sugars, caffeine and chlorogenic acid using HPLC. Generally, green embryogenic cultures contained more and higher quantities of the compounds. Glucose and fructose were highest (38.95 mg/g and 45.43 mg/g respectively) in leaf discs of brown non-embryogenic cultures. Sucrose was highest (62.15 mg/g) in embryos of green embryogenic cultures. Embryos of green embryogenic cultures had the highest chlorogenic acid (5.3 mg/g), whereas caffeine was highest (0.55 mg/g) in embryos of brown embryogenic cultures. Endogenous fructose and glucose inhibited embryogenesis, while sucrose, chlorogenic acids and caffeine promoted embryogenesis and are potential biomarkers for embryogenesis. Other biochemical compounds such as organic acids should be identified and their role in coffee somatic embryogenesis determined.

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Section: Composition Of the Coffee Seedmentioning

confidence: 99%

Evaluation of Endogenous Sugars, Chlorogenic Acid and Caffeine Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)

Isutsa

Mayoli

Nyende

et al. 2018

JAA

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“…Guarana is the plant with the highest CF content ever described in the literature, ranging from 2.5 to 6.98% in dry seeds (Ashihara et al., 2017; Escobar et al., 1985; Lyra, 1953; Schimpl et al., 2014; Spoladore, Boaventura, & Saes, 1987). The highest concentrations of catechins in guarana are those of (+)‐catechin (2 R ,3 S ‐3,5,7,3′,4′‐pentahydroxyflavan) and (−)‐epicatechin (2 R ,3 R ‐3,5,7,3′,4′‐pentahydroxyflavan) (Hollman & Arts, 2000).…”

Section: Introductionmentioning

confidence: 99%

Phytochemistry divergence among guarana genotypes as a function of agro‐industrial characters

et al. 2020

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Guarana [Paullinia cupana var. sorbilis (Mart.) Ducke] is a typical South American plant, native to the Amazon, with subspontaneous occurrence in Maués, Amazonas, Brazil, where it was domesticated and cultivated by the Sateré‐Mawé indigenous community. Guarana is an original Brazilian product with great economic and industrial potential owing to the energetic, antioxidant, and medicinal properties of its seeds. In spite of its importance, there is still a gap in knowledge about genetic diversity and adaptations that might improve agro‐industrial uses of guarana seeds. This study was aimed at estimating phytochemical diversity, classification, and typology of guarana genotypes. Agro‐industrial potential among eight genotypes was estimated based on phytochemical characters (theobromine, caffeine, catechin, and epicatechin content, quantified by high performance liquid chromatography) and yield in three locations from harvests between 2015 and 2016. Phytochemical divergence was assessed by the mean Euclidean distance. Grouping was performed by the unweighted pair‐group method using arithmetic averages. Metabolite content varied considerably between genotypes (e.g., among caffeine [3.23–7.35%], catechin [0.20–3.57%], and epicatechin [0.09–4.22%]), and dry seed yield was the character with the greatest variation (0.048–5.13 kg plant−1). The metabolic profile variations of the genotypes were clustered into three chemotypes: energetic and antioxidant guarana (genotypes CIR815, CIR904, and CMA498); antioxidant guarana (genotypes BRS‐Maués and CMU874); and energetic guarana (genotypes CMA831, CMU952, and BRS‐CG372). Genotypes CIR815, CIR904, and CMU874 have the potential to be future commercial cultivars, providing quality raw material for soft drinks, energy drinks, and pharmaceutical products.

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“…O guaraná é a espécie na qual foi encontrado o maior conteúdo de cafeína já descrito em vegetais. Suas sementes podem conter de 2,5 a 6% de cafeína (Spoladore, 1987;Oliveira, 2010). As outras metilxantinas, como teobromina e teofilina, também foram encontradas em tecidos de guaraná, mas em menor proporção, abaixo de 0,3% (Wisniewski, 1955;Oliveira, 2010).…”

Section: Cafeínaunclassified

“…No Brasil, o cafeeiro é quem recebe destaque, por este ser um dos principais produtos agrícolas na pauta das exportações do país. Por outro lado, a cafeína do guaraná ainda é pouco investigada, apesar de ser a fonte vegetal mais rica desse alcaloide (Spoladore, 1987;Mehr et al, 1996;Ashihara et al, 2011;Oliveira, 2010;).…”

Section: Cafeínaunclassified

“…Devido à elevada quantidade de cafeína, a semente de guaraná recebe maior atenção no que se refere a estudos de conteúdo de metilxantinas. Os níveis de cafeína encontrados nos frutos de guaraná das cultivares aqui estudadas (figura 10) estão de acordo com a literatura, que descreve teores entre 2,5 a 6% para sementes e de zero a 1% no pericarpo (Pires, 1949;Maravalhas, 1965;Spoladore, 1987;Baumann et al, 1995;Oliveira, 2010;Ashihara et al, 2011). A predominância de cafeína nas sementes de guaraná em contraste com o acúmulo de teobromina nos demais tecidos sugere uma adaptação à condições de pressão do ambiente, podendo ser uma especiação para defesa de predadores específicos desse tecido (Mithöfer & Boland, 2012;Weckerle et al, 2003).…”

Section: Distribuição De Cafeína Nos Tecidos De Guaranáunclassified

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Teores de metilxantinas e metabolismo de cafeína em frutos de guaraná (Paullinia cupanavar. sorbilisKunth.)

Schimpl¹,

Mazzafera²

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FLÁVIA CAMILA SCHIMPL "TEORES DE METILXANTINAS E METABOLISMO DE CAFEÍNA EM FRUTOS DE GUARANÁ (Paullinia cupana var. sorbilis Kunth.)" CAMPINAS 2013 ii iii iv v vi DEDICATÓRIA Dedico este trabalho ao meu esposo João Cláudio e aos meus pais Ana Lúcia e Edvaldo, como todo carinho. vii AGRADECIMENTOSAo Programa de Pós-Graduação em Biologia Vegetal.À Fapesp, pelo suporte financeiro.Ao Departamento de Biologia Vegetal, pela infra-estrutura, e a todos do departamento, técnicos, alunos e professores.Ao professor Paulo Mazzafera, meu orientador, pela oportunidade de crescimento e aprendizado durante a execução do trabalho.Ao professor José Francisco de Carvalho Gonçalves do Instituto Nacional de Pesquisas da Amazônia pela indicação, confiança e apoio na coleta do material vegetal em Manaus.Aos colegas do Laboratório de Fisiologia e Bioquímica Vegetal -INPA, Rebeca, Rodrigo e Mebly pelo apoio nas coletas.Ao professor José Ferreira da Silva da Universidade Federal do Amazonas, pelo contato com a Fazenda Agropecuária Jayoro Ltda e informações valiosas a respeito do guaraná.À Fazenda Agropecuária Jayoro Ltda pela disponibilidade do material vegetal. À Dra. Juliana Lischka Sampaio Mayer pela ajuda nas análises de hibridização in situ.Ao Eduardo Kiyota pela ajuda nas análises de expressão e purificação da proteína recombinante e amizade.Ao Dr. Fábio Conte pela ajuda nas análises de purificação da proteína heteróloga.Aos membros do LaFiMP-Unicamp pela ajuda constante com diferentes técnicas, discussões científicas e companheirismo.À Juliana Cazoto e Adilson Domingues, meus amigos, por estarem sempre dispostos a me ajudar análises e tira dúvidas, além de tornarem esses dois anos mais alegres.Aos membros da pré-banca e banca, pelas valiosas sugestões ao meu trabalho.À minha família, pais, irmãs, cunhados e sobrinhos pelo apoio incondicional e refúgio nos momentos de dificuldades.Ao meu esposo João Cláudio pelo apoio, incentivo, paciência, companheirismo, amizade e claro, amor. E à sua família, por suportar a saudade. À todos os meus amigos e pessoas que, de qualquer forma, colaboraram para a realização deste trabalho.

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Teor de cafeína em sementes matrizes do guaranazeiro

Cited by 11 publications

References 1 publication

Evaluation of Endogenous Sugars, Chlorogenic Acid and Caffeine Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)

Evaluation of Endogenous Sugars, Chlorogenic Acid and Caffeine Associated with Direct Somatic Embryogenesis of Coffee (Coffea arabica L.)

Phytochemistry divergence among guarana genotypes as a function of agro‐industrial characters

Teores de metilxantinas e metabolismo de cafeína em frutos de guaraná (Paullinia cupanavar. sorbilisKunth.)

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