Tension-stabilized pores in giant vesicles: determination of pore size and pore line tension

Zhelev, Doncho V.; Needham, David

doi:10.1016/0005-2736(93)90319-u

Cited by 310 publications

(318 citation statements)

References 35 publications

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“…As shown in Figs. 3 and 4a, lysis-inducing TP (indicated as horizontal dashed line at 1.1 V where ET equals 5.7 mN/m (Riske and Dimova 2005;Zhelev and Needham 1993) resulted from an applied voltage of 20 V and 0.1 µm cleft size. In Fig.…”

Section: Microelectrotension Strain and Stress Values Range From Phmentioning

confidence: 99%

“…Because the membrane is incompressible, electrocompression leads to electrotension (ET) (Needham and Hochmuth 1989;Riske and Dimova 2005), which forms the basis of electroporation, a widely-used method by which molecules are inserted into cells through electric field-induced membrane pores (Weaver 1993(Weaver , 1995. Electroporation occurs when the transmembrane potential (TP) reaches 0.2-1.0 V (Akinlaja and Sachs 1998;Kinosita Jr. and Tsong 1979;Teissie et al 2005;Weaver 1993) resulting in membrane tensions exceeding 1 mN/m (Zhelev and Needham 1993). After pore formation, extracellular molecules enter the cell electrokinetically and diffusively.…”

Section: Introductionmentioning

confidence: 99%

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Finite element analysis of microelectrotension of cell membranes

Bae

Butler

2007

Biomech Model Mechanobiol

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Electric fields can be focused by micropipette-based electrodes to induce stresses on cell membranes leading to tension and poration. To date, however, these membrane stress distributions have not been quantified. In this study, we determine membrane tension, stress, and strain distributions in the vicinity of a microelectrode using finite element analysis of a multiscale electro-mechanical model of pipette, media, membrane, actin cortex, and cytoplasm. Electric field forces are coupled to membranes using the Maxwell stress tensor and membrane electrocompression theory. Results suggest that micropipette electrodes provide a new non-contact method to deliver physiological stresses directly to membranes in a focused and controlled manner, thus providing the quantitative foundation for micreoelectrotension, a new technique for membrane mechanobiology.

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Section: Microelectrotension Strain and Stress Values Range From Phmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Finite element analysis of microelectrotension of cell membranes

Bae

Butler

2007

Biomech Model Mechanobiol

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“…4). The rationale for maintaining the lower steady level of shear stress following the initial impulse comes from electroporation studies of Zhelev and Needham (1993), which showed that membrane tension could stabilize membrane pores. Again, the goal of this model was to produce the membrane permeability change observed in vivo.…”

Section: Injury Protocolmentioning

confidence: 99%

The Effect of Poloxamer-188 on Neuronal Cell Recovery from Mechanical Injury

Serbest

Horwitz

Barbee

2005

Journal of Neurotrauma

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Neuronal injury resulting from mechanical deformation is poorly characterized at the cellular level. The immediate structural consequences of the mechanical loading lead to a variety of inter-and intra-cellular signaling events that interact on multiple time and length scales. Thus, it is often difficult to establish cause-and-effect relationships such that appropriate treatment strategies can be devised. In this report, we showed that treating mechanically injured neuronal cells with an agent that promotes the resealing of disrupted plasma membranes rescues them from death at 24 h post-injury. A new in vitro model was developed to allow uniform mechanical loading conditions with precisely controlled magnitude and onset rate of loading. Injury severity increased monotonically with increasing peak shear stress and was strongly dependent on the rate of loading as assessed with the MTT cell viability assay, 24 h post-injury. Mechanical injury produced an immediate disruption of membrane integrity as indicated by a rapid and transient release of LDH. For the most severe injury, cell viability decreased approximately 40% with mechanical trauma compared to sham controls. Treatment of cells with Poloxamer 188 at 15 min post-injury restored long-term viability to control values. These data establish membrane integrity as a novel therapeutic target in the treatment of neuronal injury.

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“…The overall shape of the free-energy barrier for pore nucleation shows no indication for the existence of a metastable intermediate during pore The formation of pores in bilayer membranes plays a role in many biological and biomimetic systems. [1][2][3] The simplest model for pore formation in membranes is based on classical nucleation theory ͑CNT͒. 4 In this picture, the formation of pores in membranes is an activated process that is controlled by the competition between the surface tension of the membrane and the line tension associated with the rim of the pore.…”

mentioning

confidence: 99%

“…[1][2][3] The simplest model for pore formation in membranes is based on classical nucleation theory ͑CNT͒. 4 In this picture, the formation of pores in membranes is an activated process that is controlled by the competition between the surface tension of the membrane and the line tension associated with the rim of the pore.…”

mentioning

confidence: 99%

Pore nucleation in mechanically stretched bilayer membranes

Wang

Frenkel

2005

The Journal of Chemical Physics

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We report a computer-simulation study of the free-energy barrier for the nucleation of pores in the bilayer membrane under constant stretching lateral pressure. We find that incipient pores are hydrophobic but as the lateral size of the pore nucleus becomes comparable with the molecular length, the pore becomes hydrophilic. In agreement with previous investigations, we find that the dynamical process of growth and closure of hydrophilic pores is controlled by the competition between the surface tension of the membrane and the line tension associated with the rim of the pore. We estimate the line tension of a hydrophilic pore from the shape of the computed free-energy barriers. The line tension thus computed is in a good agreement with available experimental data. We also estimate the line tension of hydrophobic pores at both macroscopic and microscopic levels. The comparison of line tensions at these two different levels indicates that the "microscopic" line tension should be carefully distinguished from the "macroscopic" effiective line tension used in the theoretical analysis of pore nucleation. The overall shape of the free-energy barrier for pore nucleation shows no indication for the existence of a metastable intermediate during pore The formation of pores in bilayer membranes plays a role in many biological and biomimetic systems. [1][2][3] The simplest model for pore formation in membranes is based on classical nucleation theory ͑CNT͒. 4 In this picture, the formation of pores in membranes is an activated process that is controlled by the competition between the surface tension of the membrane and the line tension associated with the rim of the pore. Based on this model, several theoretical investigations have been carried out to analyze the structural and dynamical properties of pores in membranes. [5][6][7][8][9][10] In order to observe pore formation in realistic models for phospholipid bilayers 11 on the time scale of a simulation ͑nanoseconds͒, very large stresses ͑or very large electric fields͒ are required. Coarse-grained simulations offer the possibility to perform a systematic study of the size and shape distribution of pores that appear spontaneously in a membrane at thermal equilibrium. 12 Using such studies, it is possible to estimate the line tension associated with the rim of the pore, 13 and the free-energy profile of pore formation in a membrane at constant surface area. 14 In parallel, there has been much progress in the development of experimental techniques to probe the dynamical features of pore formation in membranes. 15,16 In spite of these efforts, our knowledge about the early stages of pore nucleation is still limited. Yet, experiments by Evans et al. 16 suggest that, precisely in this regime, something interesting happens. In Ref. 16, the rupture rate of spherical vesicles is probed as a function of the rate at which the tension is increased. These experiments suggest that pore nucleation is a two-stage process: presumably, in the early stages a molecular-size metastable defect f...

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Tension-stabilized pores in giant vesicles: determination of pore size and pore line tension

Cited by 310 publications

References 35 publications

Finite element analysis of microelectrotension of cell membranes

Finite element analysis of microelectrotension of cell membranes

The Effect of Poloxamer-188 on Neuronal Cell Recovery from Mechanical Injury

Pore nucleation in mechanically stretched bilayer membranes

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