Tensiometric and Phase Domain Behavior of Lung Surfactant on Mucus-like Viscoelastic Hydrogels

Abstract

Lung surfactant has been observed at all surfaces of the airway lining fluids and is an important contributor to normal lung function. In the conducting airways, the surfactant film lies atop a viscoelastic mucus gel. In this work, we report on the characterization of the tensiometric and phase domain behavior of lung surfactant at the air-liquid interface of mucus-like viscoelastic gels. Poly(acrylic acid) hydrogels were formulated to serve as a model mucus with bulk rheological properties that matched those of tracheobronchial mucus secretions. Infasurf® (Calfactant), a commercially available pulmonary surfactant derived from calf lung extract, was spread onto the hydrogel surface. The surface tension lowering ability and relaxation of Infasurf films on the hydrogels was quantified and compared to Infasurf behavior on an aqueous subphase. Infasurf phase domains during surface compression were characterized by fluorescence microscopy and phase shifting interferometry. We observed that increasing the bulk viscoelastic properties of the model mucus hydrogels reduced the ability of Infasurf films to lower surface tension and inhibited film relaxation. A shift in the formation of Infasurf condensed phase domains from smaller, more spherical domains to large, agglomerated, multilayer structures was observed with increasing viscoelastic properties of the subphase. These studies demonstrate that the surface behavior of lung surfactant on viscoelastic surfaces, such as those found in the conducting airways, differs significantly from aqueous, surfactant-laden systems.

Phosphatidylglycerol and surfactant: A potential treatment for COVID-19?

“… ƒ Discovery Labs became Windtree Therapeutics in 2016. † Abbreviations: FDA, Food and Drug Administration; GSK, GlaxoSmithKline; N/A, not applicable; PG, phosphatidylglycerol; PL, phospholipid; SP-B, surfactant protein-B; SP-C, surfactant protein-C. § The values for protein and PG amounts were obtained from references [49] , [71] , [72] , [73] . …”

Thermo‐physical properties of synthetic mucus for the study of airway clearance

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Nanometric Surface Oscillation Spectroscopy of Water-Poor Microemulsions

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