We report work in which an atomic force microscope (AFM) is used to stretch (and ultimately, to rupture) a thin¯lm of liquid between a moving colloid sphere and a static plane surface. Under some circumstances, when the sphere and the surface are pulled apart su±ciently rapidly, an unexpected transient decrease in the sphere{ surface separation is recorded. The results of numerical simulations of cavitation bubble dynamics suggest that the growth of a cavitation bubble within a liquid may result in the development of su±ciently large negative pressures to account for this phenomenon. The results of separate experiments, which involve acoustic pulse propagation within metre-long columns of liquid and high-speed microphotography (using a novel optical system designed for this work), are used to show that the peak tensile forces recorded in the AFM experiments correspond to the development of tensile stresses that are commensurate with the°uid's e®ective tensile strength (or cavitation threshold'). The results of this study, which, to the best of our knowledge, is the¯rst to apply the AFM in cavitation bubble dynamics work, provide evidence that, in the cavitation of liquids within con¯ned spaces, the growth of a cavity may be more damaging than its subsequent collapse.