Tensile properties of synthetic pyriform spider silk fibers depend on the number of repetitive units as well as the presence of N- and C-terminal domains

Zhu, Hongnian; Rising, Anna; Johansson, Jan; Zhang, Xiaohua; Lin, Ying; Zhang, Lei; Yi, Tuo; Mi, Junpeng; Meng, Qing

doi:10.1016/j.ijbiomac.2020.03.042

Cited by 33 publications

(27 citation statements)

References 56 publications

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“…The curves show that the distribution of hydrophilic and hydrophobic amino acid sequences in R is greater even than in W. The size of R accounts for its increased proportion in the iNnRC spidroins, and the NT and CT are both relatively highly soluble. These features together contribute to the higher solubility of iNnRC spidroins (with 2-4 Rs) compared to NTW 1-4 CT (shown in Figure 2 b) and the other reported recombinant spidroins with similar molecular weights [ 33 ].…”

Section: Resultsmentioning

confidence: 89%

Novel Highly Soluble Chimeric Recombinant Spidroins with High Yield

Jia

Meng

2020

IJMS

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Spider silk has been a hotspot in the study of biomaterials for more than two decades due to its outstanding mechanical properties. Given that spiders cannot be farmed, and their low silk productivity, many attempts have been made to produce recombinant spidroins as an alternative. Herein, we present novel chimeric recombinant spidroins composed of 1 to 4 repetitive units of aciniform spidroin (AcSp) flanked by the nonrepetitive N- and C-terminal domains of the minor ampullate spidroin (MiSp), all from Araneus ventricosus. The spidroins were expressed in the form of inclusion body in E. coli with high yield. Remarkably, the aqueous solubility of the four spidroins ranged from 13.4% to over 50% (m/v). The four spidroins could self-assemble into silk-like fibers by hand-drawing. The secondary structures of these proteins, determined by circular dichroism spectrum (CD) and Fourier transform infrared spectrum (FTIR), indicated a prominent transformation from α-helix to β-sheet after fiber formation. The mechanical properties of the hand-drawn fibers showed a positive correlation with the spidroin molecular weight. In summary, this study describes promising biomaterials for further study and wide application.

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Section: Resultsmentioning

confidence: 89%

Novel Highly Soluble Chimeric Recombinant Spidroins with High Yield

Jia

Meng

2020

IJMS

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“…In addition, the use of synthetic biology enables the design and production of novel recombinant spidroins tailored towards specific functions in addition to those required by spiders [11]. Recombinant spidroins can also serve as model proteins in the investigation of structure-function relationships of native silk proteins [12][13][14].…”

Section: The Allure Of Spider Silkmentioning

confidence: 99%

Host Systems for the Production of Recombinant Spider Silk

Whittall

Baker

Breitling

et al. 2021

Trends in Biotechnology

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Spider silk is renowned for its impressive mechanical properties. It is one of the strongest known biomaterials, possessing mechanical properties that outmatch both steel and Kevlar. However, the farming of spiders for their silk is unfeasible. Consequently, production of recombinant spider silk proteins (spidroins) in more amenable hosts is an exciting field of research. For large-scale production to be viable, a heterologous silk production system that is both highly efficient and cost effective is essential. Genes encoding recombinant spidroin have been expressed in bacterial, yeast, insect, and mammalian cells, in addition to many other platforms. This review discusses the recent advances in exploiting an increasingly diverse range of host platforms in the heterologous production of recombinant spidroins. Highlights Recombinant spidroins of comparable size and mechanical function to native silk proteins have been successfully produced by engineered E. coli. Recombinant spidroins have been expressed in transgenic plants, rice, and alfalfa. These expression platforms can potentially offer increased industrialscale economic viability over alternative host systems.

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“…Meanwhile, the expression level of repeat units in the vector is still limited by the characteristics of the vector. 9,88,89 As shown in Figure 5, the recombinant spider silk protein could express in the different host systems. The high molecular weight spider silk protein was successfully expressed by animals and plants, but it still has the disadvantage of low expression efficiency and high cost, which needs further study.…”

Section: Artificial Spider Silk Proteinmentioning

confidence: 99%

“…8 For their peptide chains, there are mutual features in the composition and structure. Firstly, they all have five parts in primary structure: repetitive GPGXX/GGX (glycine region) and poly (A)/ poly (AG) (polyalanine) region, spacer region, and non-repetitive terminal region including carbon terminal (CT) and nitrogen terminal (NT) which are conserved in evolution 9 (Figure 2). The NT played a role of restriction on the agglomeration of silk solution before spinning, while the CT affected the switch between storage and assembly as well as the structure of nanofiber.…”

Section: Introductionmentioning

confidence: 99%

Progress in the application of spider silk protein in medicine

et al. 2021

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Spider silk protein has attracted much attention on account of its excellent mechanical properties, biodegradability, and biocompatibility. As the main protein component of spider silk, spidroin plays important role in spider spinning under natural circumstances and biomaterial application in medicine as well. Compare to the native spidroin which has a large molecular weight (>300 kDa) with highly repeat glycine and polyalanine regions, the recombinant spidroin was maintained the core amino motifs and much easier to collect. Here, we reviewed the application of recombinant spider silk protein eADF4(C16), major ampullate spidroin (MaSp), minor ampullate spidroin (MiSp), and the derivatives of recombinant spider silk protein in drug delivery system. Moreover, we also reviewed the application of spider silk protein in the field of alternative materials, repairing materials, wound dressing, surgical sutures along with advances in recombinant spider silk protein.

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Tensile properties of synthetic pyriform spider silk fibers depend on the number of repetitive units as well as the presence of N- and C-terminal domains

Cited by 33 publications

References 56 publications

Novel Highly Soluble Chimeric Recombinant Spidroins with High Yield

Novel Highly Soluble Chimeric Recombinant Spidroins with High Yield

Host Systems for the Production of Recombinant Spider Silk

Progress in the application of spider silk protein in medicine

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