Tenocyclidine treatment in soman-poisoned rats--intriguing results on genotoxicity versus protection.

Abstract

This study aimed to evaluate the antidotal potency of tenocyclidine (TCP) that probably might protect acetylcholinesterase (AChE) in the case of organophosphate poisoning. TCP was tested alone as a pretreatment or in combination with atropine as a therapy in rats poisoned with (1/4) and (1/2) of LD(50) of soman. Possible genotoxic effects of TCP in white blood cells and brain tissue were also studied. Results were compared with previous findings on the adamantyl tenocyclidine derivative TAMORF. TCP given alone as pretreatment, 5 min before soman, seems to be superior in the protection of cholinesterase (ChE) catalytic activity in the plasma than in brain, especially after administration of the lower dose of soman. Plasma activities of the enzyme after a joint treatment with TCP and soman were significantly increased at 30 min (P

Irinotecan Side Effects Relieved by the Use of HI‐6 Oxime: In Vivo Experimental Approach

“…Tissue was homogeni s ed in an ice‐cold buffer (0.075 M NaCl and 0.024 M Na 2 EDTA). The selection of the method for the tissue preparation was based on the observations from our earlier studies [14,15] that had given acceptably low background DNA damage and consistent results using the same cell types. Slides were examined at 250× magnification with a fluorescence microscope (Zeiss, Jena, Germany) using the image analysis system (Comet Assay II, Perceptive Instruments Ltd., Haverhill, UK).…”