Tendon Stem/Progenitor Cell Subpopulations and Their Implications in Tendon Biology

Huang, Zhi Wei; Yin, Zi; Xu, Jialu; Yang, Fei; Heng, Boon Chin; Jiang, Xuesheng; Chen, Weishan; Shen, Wei

doi:10.3389/fcell.2021.631272

Cited by 21 publications

(21 citation statements)

References 119 publications

(141 reference statements)

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“…It has been confirmed that Scx, Msi1, αSMA, Cd133, and Nes is present in perivascular TSPCs by immunostaining of the perivascular TSPCs isolated from human tendon microvessels ( Tempfer et al, 2009 ). A former study discovered some assertive evidence that TSPCs can also be explanted from adjoining tissues ( Huang et al, 2021 ). Besides, a study based on the zebrafish models revealed that totally ablating tendons could be well-restored by progenitors derived from neighboring tissues.…”

Section: Tendon Development Guidance For Tendon Repairmentioning

confidence: 99%

“…Inflammation reaction is an important section of the tendon healing and may ultimately affect repair outcomes. The effects of HIF-2α, Prostaglandin E2 and IL-1β on TSPCs, promote enhanced expression of tenogenic genes ( Huang et al, 2021 ). After inflammatory stimulation, both embryonic and postnatal tendon cells exhibit similar tenogenic commitment, but the latter also expressed up-regulated catabolic enzymes and inflammatory mediators ( Li et al, 2019 ).…”

Section: Tendon Development Guidance For Tendon Repairmentioning

confidence: 99%

“…After inflammatory stimulation, both embryonic and postnatal tendon cells exhibit similar tenogenic commitment, but the latter also expressed up-regulated catabolic enzymes and inflammatory mediators ( Li et al, 2019 ). In summary, TSPCs serve as the vital cells to migrate to the area of injury, proliferate and then express tendon-related, pericyte-related, and pluripotent markers to regulate the process of tendon healing and remodeling ( Huang et al, 2021 ). TSPCs from the tendon proper participate in healing of embryonic and early postnatal tendons, contributing to the complete regeneration of embryonic and early postnatal tendons.…”

Section: Tendon Development Guidance For Tendon Repairmentioning

confidence: 99%

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Comparison of Tendon Development Versus Tendon Healing and Regeneration

Ruan

Huang

et al. 2022

Front. Cell Dev. Biol.

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Tendon is a vital connective tissue in human skeletal muscle system, and tendon injury is very common and intractable in clinic. Tendon development and repair are two closely related but still not fully understood processes. Tendon development involves multiple germ layer, as well as the regulation of diversity transcription factors (Scx et al.), proteins (Tnmd et al.) and signaling pathways (TGFβ et al.). The nature process of tendon repair is roughly divided in three stages, which are dominated by various cells and cell factors. This review will describe the whole process of tendon development and compare it with the process of tendon repair, focusing on the understanding and recent advances in the regulation of tendon development and repair. The study and comparison of tendon development and repair process can thus provide references and guidelines for treatment of tendon injuries.

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Section: Tendon Development Guidance For Tendon Repairmentioning

confidence: 99%

Section: Tendon Development Guidance For Tendon Repairmentioning

confidence: 99%

Section: Tendon Development Guidance For Tendon Repairmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Tendon Development Versus Tendon Healing and Regeneration

Ruan

Huang

et al. 2022

Front. Cell Dev. Biol.

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“…TSPCs differ from MSCs for their transcriptional profiles with higher tendon-related gene expression levels ( Tan et al, 2012 ). Many advances in the identification and characterization of distinct TSPC subpopulations have been made using single-cell analyses, giving a more complete view of the TSPC identity ( Harvey et al, 2019 ; Mienaltowski et al, 2019 ; Kendal et al, 2020 ; Huang et al, 2021 ). TSPCs have attracted a lot of attention playing a key role in tendon development, homeostasis, and healing ( Bi et al, 2007 ; Millar et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Human Tendon Stem/Progenitor Cell Features and Functionality Are Highly Influenced by in vitro Culture Conditions

Orfei

Bowles

Kouroupis

et al. 2021

Front. Bioeng. Biotechnol.

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Our understanding of tendon biology continues to evolve, thus leading to opportunities for developing novel, evidence-based effective therapies for the treatment of tendon disorders. Implementing the knowledge of tendon stem/progenitor cells (TSPCs) and assessing their potential in enhancing tendon repair could fill an important gap in this regard. We described different molecular and phenotypic profiles of TSPCs modulated by culture density, as well as their multipotency and secretory activities. Moreover, in the same experimental setting, we evaluated for different responses to inflammatory stimuli mediated by TNFα and IFNγ. We also preliminarily investigated their immunomodulatory activity and their role in regulating degradation of substance P. Our findings indicated that TSPCs cultured at low density (LD) exhibited cobblestone morphology and a reduced propensity to differentiate. A distinctive immunophenotypic profile was also observed with high secretory and promising immunomodulatory responses when primed with TNFα and IFNγ. In contrast, TSPCs cultured at high density (HD) showed a more elongated fibroblast-like morphology, a greater adipogenic differentiation potential, and a higher expression of tendon-related genes with respect to LD. Finally, HD TSPCs showed immunomodulatory potential when primed with TNFα and IFNγ, which was slightly lower than that shown by LD. A shift from low to high culture density during TSPC expansion demonstrated intermediate features confirming the cellular adaptability of TSPCs. Taken together, these experiments allowed us to identify relevant differences in TSPCs based on culture conditions. This ability of TSPCs to acquire distinguished morphology, phenotype, gene expression profile, and functional response advances our current understanding of tendons at a cellular level and suggests responsivity to cues in their in situ microenvironment.

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“…However, many other genes related to bone formation remain uncharacterized during IB development. The formation of IBs may undergo several processes including differentiation of tendon stem/progenitor cells (TSPCs) into osteoblasts, ossification, and mineralization [ 3 , 18 , 19 ]. Therefore, the genes involved in these processes are worth of exploration to better understand the formation of IBs.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Myosepta Development and Transcriptome Profiling between Blunt Snout Bream with and Tilapia without Intermuscular Bones

Zhou

Chang

Chen

et al. 2021

Biology

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Intermuscular bones (IBs) are small spicule-like bones located in the myosepta of basal teleosts, which negatively affect the edibleness and economic value of fish. Blunt snout bream (Megalobrama amblycephala, with epineural and epipleural IBs) and tilapia (Oreochromis niloticus, without epineural and epipleural IBs) are two major aquaculture species and ideal models for studying the formation mechanisms of fish IBs. Here, we compared myosepta development between M. amblycephala and O. niloticus, based on histological analysis, transcriptome profiling, and expression analysis of bone-related genes. The histological results showed that dye condensation began to appear in the myosepta 20 days post hatching (dph) in M. amblycephala, and IBs could be clearly observed 50 dph in the myosepta, based on different staining methods. However, in O. niloticus, dye condensation was not observed in the myosepta from 10 to 60 dph. Differentially expressed genes (DEGs) at different developmental stages were screened by comparing the transcriptomes of M. amblycephala and O. niloticus, and KEGG analysis demonstrated that these DEGs were enriched in many bone-related pathways, such as focal adhesion, calcium, and Wnt signaling pathways. Quantitative PCR was performed to further compare the expression levels of some bone-related genes (scxa, scxb, runx2a, runx2b, bgp, sp7, col1a2, entpd5a, entpd5b, phex, alpl, and fgf23). All the tested genes (except for alpl) exhibited higher expression levels in M. amblycephala than in O. niloticus. A comparison of the dorsal and abdominal muscle tissues between the two species also revealed significant expression differences for most of the tested genes. The results suggest that scxa, scxb, runx2a, runx2b, entpd5a, col1a2, and bgp may play important roles in IB development. Our findings provide some insights into the molecular mechanisms of IB formation, as well as clues for further functional analysis of the identified genes to better understand the development of IBs.

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Tendon Stem/Progenitor Cell Subpopulations and Their Implications in Tendon Biology

Cited by 21 publications

References 119 publications

Comparison of Tendon Development Versus Tendon Healing and Regeneration

Comparison of Tendon Development Versus Tendon Healing and Regeneration

Human Tendon Stem/Progenitor Cell Features and Functionality Are Highly Influenced by in vitro Culture Conditions

Comparison of Myosepta Development and Transcriptome Profiling between Blunt Snout Bream with and Tilapia without Intermuscular Bones

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