Tendon grafting with glutaraldehyde fixed material

McMaster, William C.; Kouzelos, Jack; Liddle, Shardon; Waugh, Theodore R.

doi:10.1002/jbm.820100207

Cited by 42 publications

(3 citation statements)

References 19 publications

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“…GA cross-linked bioprostheses are not generally finally sterilized as the processing solutions are bacteriocidal (12). Some implants are stored in GA or formaldehyde to maintain sterility (10,12,23). Final sterilization was deemed necessary in this study as only those tendons prepared in the higher concentrations of GA were exposed to bacteriocidal concentrations.…”

Section: Discussionmentioning

confidence: 99%

Collagen Cross‐Linking and Resorption: Effect of Glutaraldehyde Concentration

1990

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Cross-linked collagen bioprostheses usually are designed to be inert and nonresorbable, resulting in fatigue and wear failure in high-stress environments. Eventual replacement of the implant, although minimizing strength loss during resorption, would result in a graft with reparative ability. Kangaroo tail tendon (KTT) partially cross-linked with glutaraldehyde (GA) was evaluated in vitro for resistance to bacterial collagenase digestion and in vivo for biocompatibility and resorbability in an intramuscular implant assay. Cross-linking was quantified by thermal denaturation studies. Incomplete cross-linking was achieved with concentrations of GA less than 0.1% (w/v). KTT cross-linked in greater than or equal to 0.05% GA were collagenase resistant being incompletely digested after 240 h. Cross-linking of KTT with low concentrations of GA resulted in partial collagenase resistance and slowed resorption.

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Section: Discussionmentioning

confidence: 99%

Collagen Cross‐Linking and Resorption: Effect of Glutaraldehyde Concentration

1990

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“…The resulting polypyrrole-coated substrates were rinsed with deionized water and air-dried. Following substrate preparation, a linker, glutaraldehyde, was covalently attached by treating the polypyrrole-coated substrates with 25% glutaraldehyde for 4 h, facilitating flexible bridges for effective binding of biomolecules [ 63 , 64 ]. After washing with phosphate-buffered saline (PBS), site-specific SARS-CoV-2 RBD lactam-stapled hACE-2 peptide was covalently immobilized onto the glutaraldehyde-treated substrates at 30 µg/mL concentration in 1× PBS for 16 h. Excess and unbound peptide were removed by washing with 1× Tris-buffered saline with Tween20 (TBST).…”

Section: Methodsmentioning

confidence: 99%

Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection

Meshesha,

Sardar,

Supekar

et al. 2023

Sensors

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The COVID-19 pandemic has underscored the critical need for rapid and accurate screening and diagnostic methods for potential respiratory viruses. Existing COVID-19 diagnostic approaches face limitations either in terms of turnaround time or accuracy. In this study, we present an electrochemical biosensor that offers nearly instantaneous and precise SARS-CoV-2 detection, suitable for point-of-care and environmental monitoring applications. The biosensor employs a stapled hACE-2 N-terminal alpha helix peptide to functionalize an in situ grown polypyrrole conductive polymer on a nitrocellulose membrane backbone through a chemical process. We assessed the biosensor’s analytical performance using heat-inactivated omicron and delta variants of the SARS-CoV-2 virus in artificial saliva (AS) and nasal swab (NS) samples diluted in a strong ionic solution, as well as clinical specimens with known Ct values. Virus identification was achieved through electrochemical impedance spectroscopy (EIS) and frequency analyses. The assay demonstrated a limit of detection (LoD) of 40 TCID50/mL, with 95% sensitivity and 100% specificity. Notably, the biosensor exhibited no cross-reactivity when tested against the influenza virus. The entire testing process using the biosensor takes less than a minute. In summary, our biosensor exhibits promising potential in the battle against pandemic respiratory viruses, offering a platform for the development of rapid, compact, portable, and point-of-care devices capable of multiplexing various viruses. The biosensor has the capacity to significantly bolster our readiness and response to future viral outbreaks.

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“…One study demonstrated the strength of stabilized collagen as well as its resistance to collagenase attack (18). Evaluation of glutaraldehydefixed tendon materials demonstrated biologic acceptability when implanted subcutaneously or as tendon grafts (17,20). Glutaraldehyde-stabilized material was shown to function suitably as a prosthesis in an evaluation of the replacement of the medial collateral ligament in a rabbit model (19).…”

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confidence: 99%

Bovine xenograft collateral ligament replacement in the dog

McMaster

1985

Journal Orthopaedic Research

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A study of the use of glutaraldehyde-stabilized bovine xenograft material as a collateral ligament replacement in 16 dogs has been done. Six xenograft implant complexes harvested 4 months postoperatively failed in tension at 772.2 +/- 463.5 versus 799.7 +/- 162.7 N (+/- 1 SD) for controls (p greater than 0.05, paired t test). Histologic evaluation in 10 dogs after implants of up to 1 year duration demonstrated a progressive invasion of the xenograft by host tissues. Xenograft remnants were easily identifiable at 1 year. The host tissues invaded in parallel to the passive collagen scaffolding of the xenograft and consisted of vessels and fibroblastic elements that produced collagen of host origin.

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Tendon grafting with glutaraldehyde fixed material

Cited by 42 publications

References 19 publications

Collagen Cross‐Linking and Resorption: Effect of Glutaraldehyde Concentration

Collagen Cross‐Linking and Resorption: Effect of Glutaraldehyde Concentration

Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection

Bovine xenograft collateral ligament replacement in the dog

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