Tendon-bioinspired wavy nanofibrous scaffolds provide tunable anisotropy and promote tenogenesis for tendon tissue engineering

Wu, Shaohua; Liu, Jiao; Qi, Ye; Cai, Jiangyu; Zhao, Jinzhong; Duan, Bin; Chen, Shaojuan

doi:10.1016/j.msec.2021.112181

Cited by 32 publications

(18 citation statements)

References 57 publications

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“…SF was extracted following our previous experimental protocols [ 33 , 34 ]. Briefly, Bombyx mori silk cocoons were cut into dime-sized pieces that were degummed with boiling sodium carbonate solution, and the as-degummed silk materials were squeezed and dried overnight in room temperature.…”

Section: Methodsmentioning

confidence: 99%

Electrospun strong, bioactive, and bioabsorbable silk fibroin/poly (L-lactic-acid) nanoyarns for constructing advanced nanotextile tissue scaffolds

Liu

Zhang

et al. 2022

Materials Today Bio

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Section: Methodsmentioning

confidence: 99%

Electrospun strong, bioactive, and bioabsorbable silk fibroin/poly (L-lactic-acid) nanoyarns for constructing advanced nanotextile tissue scaffolds

Liu

Zhang

et al. 2022

Materials Today Bio

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“…Previous studies also demonstrated that the cells could sense the nanofibrous morphology and structure of scaffolds and remodel their shape and orientation accordingly. 60,61…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies also demonstrated that the cells could sense the nanofibrous morphology and structure of scaffolds and remodel their shape and orientation accordingly. 60,61 An MTT assay was further employed to determine the cell proliferation when seeded on the five different nanofibrous meshes for 14 days (Figure 8(b)). It was found that the hADMSCs seeded on all the five different meshes presented a continuous proliferation tendency during the whole culture period.…”

Section: Cell Viability and Proliferation Of Hadmscs Seeded On Variou...mentioning

confidence: 99%

Electrospun hybrid nanofibrous meshes with adjustable performance for potential use in soft tissue engineering

Zhai

Sun

et al. 2021

Textile Research Journal

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Electrospun nanofibrous scaffolds have gained extensive attention in the fields of soft tissue engineering and regenerative medicine. In this study, a series of biodegradable nanofibrous meshes were fabricated by electrospinning poly(ε-caprolactone) (PCL) and poly( p-dioxanone) (PPDO) blends with various mass ratios. All the as-developed PCL/PPDO nanofibrous meshes possessed smooth and highly aligned fiber morphology. The mean fiber diameter was 521.5 ± 76.6 nm for PCL meshes and 485.8 ± 88.9 nm for PPDO meshes, and the mean fiber diameter seemed to present a decreasing tendency with the increasing of the PPDO component. For pure PCL meshes, the contact angle was about 117.5 ± 1.6°, the weight loss ratio was roughly 0.2% after 10 weeks of degradation, and the tensile strength was 41.2 ± 2.3 MPa in the longitudinal direction and 4.2 ± 0.1 MPa in the transverse direction. It was found that the surface hydrophilicity and in vitro degradation properties of PCL/PPDO meshes apparently increased, but the mechanical properties of PCL/PPDO meshes obviously decreased when more PPDO component was introduced. The biological tests showed that 4:1 PCL/PPDO nanofibrous meshes and 1:1 PCL/PPDO nanofibrous meshes could obviously promote the adhesion and proliferation of human adipose derived mesenchymal stem cells more than pure PCL and PPDO meshes and 1:4 PCL/PPDO meshes. The results demonstrated that it is feasible to adjust the surface hydrophilicity, degradation profile, and mechanical properties as well as biological properties of as-obtained nanofibrous meshes by blending PCL and PPDO components. This study provides meaningful reference and guidance for the design and development of PCL/PPDO hybrid nanofibrous scaffolds for soft tissue engineering research and application.

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“…Similar to native tissues, the normal behaviour of tissue-engineered structures is in part dictated by the appropriate mechanical properties of biomaterial scaffolds which, for soft connective tissue conditions, are nonlinear and often anisotropic [11][12][13] . To date, various methods such as melt electrowriting (MEW) 14 , electrospinning 15,16 , textile technologies 17 , and biomechanical conditioning of cell-laden hydrogels 18 have been utilized to recapitulate some aspects of soft connective tissues' mechanical properties; however, creating the entirety of their stress-strain behaviour remains a challenge. This particularly stems from the complexity of mimicking soft connective tissue ECM microarchitecture, mainly crimped fibrous patterns, which is responsible for tissue nonlinear stress-strain behaviour.…”

Section: Mainmentioning

confidence: 99%

Programmable melt electrowriting to engineer soft connective tissues with prescribed, biomimetic biaxial mechanical properties

Mirani

Mathew

Latifi

et al. 2022

Preprint

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Appropriate load-bearing function of soft connective tissues is provided by their nonlinear and often anisotropic mechanical properties. Recapitulating this complex mechanical behaviour in tissue-engineered structures is particularly crucial, as deviation from normal native tissue mechanics can trigger pathological biomechanical pathways, leading to adverse tissue remodelling and dysfunction. Here we report a novel method combining computational modelling, melt electrowriting (MEW), and design of experiments (DOE) to generate scaffolds composed of sinusoidal fibres with prescribed biaxial tensile mechanical properties, recapitulating the distinct nonlinear, anisotropic stress-strain behaviour of three model soft connective tissues: adult aortic valve, pediatric pulmonary valve, and pediatric pericardium. The fibrous scaffolds were fabricated using MEW of polycaprolactone, and their architectures were optimized using DOE and regression models for superior control over scaffold architecture to yield prescribed mechanics. Cell-laden fibrin hydrogel was cast within the fibrous scaffolds to generate hybrid tissue sheets, suitable for connective tissue engineering. The composition of the cell-laden hydrogel and its cell density were optimized to cause minimal contraction, support high cell viability and growth, and minimally contribute to the hybrid tissue biaxial mechanical properties, which were governed by the prescribed MEW scaffold architecture. This high-fidelity approach recapitulates biaxial mechanical properties over a broad range of mechanical nonlinearity and anisotropy and is generalizable for programmed fabrication in a variety of textile, biomaterial, and tissue engineering applications.

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Tendon-bioinspired wavy nanofibrous scaffolds provide tunable anisotropy and promote tenogenesis for tendon tissue engineering

Cited by 32 publications

References 57 publications

Electrospun strong, bioactive, and bioabsorbable silk fibroin/poly (L-lactic-acid) nanoyarns for constructing advanced nanotextile tissue scaffolds

Electrospun strong, bioactive, and bioabsorbable silk fibroin/poly (L-lactic-acid) nanoyarns for constructing advanced nanotextile tissue scaffolds

Electrospun hybrid nanofibrous meshes with adjustable performance for potential use in soft tissue engineering

Programmable melt electrowriting to engineer soft connective tissues with prescribed, biomimetic biaxial mechanical properties

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