Tenascin-C derived signaling induces neuronal differentiation in a three-dimensional peptide nanofiber gel

Sever, Melike; Günay, Gökhan; Güler, Mustafa O.; Tekinay, Ayşe B.

doi:10.1039/c7bm00850c

Cited by 26 publications

(19 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mitochondrial COX subunit 7A2, tumorous imaginal disc long isoform, and an unknown protein showed changes in opposite directions in 2 independent experiments. The differentially expressed proteins were involved in many pathological and physiological processes, such as energy metabolism, purine metabolism, material transport across the membrane, lipid metabolism, protein degradation, hydrolase activity, synaptic vesicle circulation, and so on (20,21).…”

Section: Discussionmentioning

confidence: 99%

Study on the differential proteomics of rat hippocampal mitochondria during deep hypothermic circulatory arrest

et al. 2021

View full text Add to dashboard Cite

Background: This study aimed to investigate the effect of deep hypothermic circulatory arrest (DHCA) on rat hippocampal mitochondrial protein expression and its differential proteomics, and explore the potential mechanisms behind the effect.Methods: We used internal jugular vein reflux and tail artery perfusion methods to establish the rat cardiopulmonary bypass (CPB) model. Rats were dissected to obtain the hippocampus, and the hippocampal mitochondria were purified. The mitochondrial morphology and the mitochondrial marker cytochrome C oxidase (COX) qualitatively examined via transmission electron microscopy and western-blot analysis, respectively. The qualified samples were subjected to isobaric tags for relative and absolute quantification (iTRAQ); we then established the CPB model again to obtain the rat hippocampus for cryoultramicrotomy, and used immunofluorescent double staining technique to qualitatively and semi-quantitatively verify two representative differentially expressed proteins.Results: By searching the Mascot 2.2 database, 29 differentially expressed proteins were obtained with statistical significance, including 21 known proteins and 8 unknowns. The expression level of COX and monoacylglycerol lipase did not change significantly (P>0.05) during the hyperacute phase; however, their intracellular localizations were altered.Conclusions: DHCA induced the differential expression of 29 rat hippocampal mitochondrial proteins, some of which had altered intracellular localization. We speculated that the localized alteration of these proteins is one of the neuroprotection mechanisms that occurs during DHCA.

show abstract

Section: Discussionmentioning

confidence: 99%

Study on the differential proteomics of rat hippocampal mitochondria during deep hypothermic circulatory arrest

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Several short peptide sequences derived from ECM proteins have been used to induce mammalian cell differentiation in culture. For example, the laminin-derived Ile-Lys-Val-Ala-Val (IKVAV) and Tyr-Ile-Gly-Ser-Arg (YIGSR), as well as the fibronectin-derived Arg-Gly-Asp (RGD), are the most commonly used sequences to promote neural cell adhesion, migration and differentiation in vitro [30]. Also, collagen-derived dipeptides containing Pro-Hyp promote osteoblastic MC3T3 cell differentiation through upregulation of osteogenic Foxg1 expression [31].…”

Section: Discussionmentioning

confidence: 99%

Basement membrane proteins as a substrate for efficient Trypanosoma brucei differentiation in vitro

Rojas

Cayla

Matthews

2021

Preprint

View full text Add to dashboard Cite

The ability to reproduce the developmental events of trypanosomes that occur in their mammalian host in vitro offers significant potential to assist in understanding of the underlying biology of the process. For example, the transition from bloodstream slender to bloodstream stumpy forms is a quorum-sensing response to the parasite-derived peptidase digestion products of environmental proteins. As an abundant physiological substrate in vivo , we studied the ability of a basement membrane matrix enriched gel (BME) in the culture medium to support differentiation of pleomorphic Trypanosoma brucei to stumpy forms . BME comprises extracellular matrix proteins, which are among the most abundant proteins found in connective tissues in mammals and known substrates of parasite-released peptidases. We previously showed that two of these released peptidases are involved in generating a signal that promotes slender-to-stumpy differentiation. Here, we tested the ability of basement membrane extract to enhance parasite differentiation through its provision of suitable substrates to generate the quorum sensing signal, namely oligopeptides. Our results show that when grown in the presence of BME, T. brucei pleomorphic cells arrest at the G0/1 phase of the cell cycle and express the differentiation marker PAD1, the response being restricted to differentiation-competent parasites. Further, the stumpy forms generated in BME medium are able to efficiently proceed onto the next life cycle stage in vitro , procyclic forms, when incubated with cis-aconitate, further validating the in vitro BME differentiation system. Hence, BME provides a suitable in vitro substrate able to accurately recapitulate physiological parasite differentiation without the use of experimental animals.

show abstract

“…The natural ECM encompasses a 3D network of intertwined protein nanofibers which contains complex biomolecules for communication between cells . Short peptide‐based 3D nanostructured supramolecular hydrogels are excellent candidates for ECM mimicry as they provide networks of fibers which resemble the ECM structure . Modifications of certain oligopeptide termini with bio‐active ligands, aimed to introduce bio‐active components into the system, have also been reported .…”

Section: Figurementioning

confidence: 99%

Composite of Peptide‐Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio‐Inspired Soft Material

Chakraborty

Ghosh

Schnaider

et al. 2019

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Engineering of extracellular matrix (ECM) mimics, with most of the necessary features of a natural ECM, is a crucial requirement for the design of biomaterials. The natural ECM encompasses a 3D network of intertwined protein nanofibers which contains complex biomolecules for communication between cells.[1] Short peptide-based 3D nanostructured supramolecular hydrogels are excellent candidates for ECM mimicry as they provide networks of fibers which resemble the ECM structure. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] Modifications of certain oligopeptide termini with bio-active ligands, aimed to introduce bio-active components into the system, have also been reported. [22][23][24][25] The tripeptide sequence Arg-Gly-Asp (RGD), which was first recognized in fibronectin as an independent cell attachment site, is the most commonly used bio-active ligand. [26] This tri-peptide sequence is recognized by the α v β 3 and α 5 β 1 integrins located in cell membranes, thus facilitating the coupling of the ECM with the cytoskeleton. [27] Owing to these attributes, the RGD ligand has been introduced into supramolecular or polymeric substrates to facilitate cell attachment. [28][29][30][31] Fmoc-RGD [Fmoc = N-(fluorenyl-9-methoxycarbonyl)] is one of the simplest RGD derivatives capable of forming hydrogels with an inter-connected fibrous network. [15,27] Ulijn's group reported Fmoc-RGD based supramolecular hydrogels in neutral aqueous solution by appropriate mixing of two short peptidebased building blocks, Fmoc-FF (F = phenylalanine) and Fmoc-RGD and utilized them as scaffolds for cell culture. [15] They proposed that Fmoc-RGD alone was not able to form β-sheet fibrils, although in the composite gels of Fmoc-FF and Fmoc-RGD (10-30 wt% Fmoc-RGD), signatures of β-sheet structures were found. We later reported flanking of the RGD sequence by aromatic moieties, demonstrating the hydrogelation of RGD derivatives, including Fmoc-FRGD and Fmoc-RGDF. [32] Hamley and co-workers showed that the simple Fmoc-RGD moiety, without any modification or mixing with other hydrogelator peptides, could from nano-fibrous hydrogels. [27] Castelletto and co-workers prepared monoliths of Fmoc-RGD gels at a high peptide concentration of 10 wt% which showed excellent Bio-Inspired Soft MaterialsPeptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery. The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion. Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture. In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrog...

show abstract

Tenascin-C derived signaling induces neuronal differentiation in a three-dimensional peptide nanofiber gel

Cited by 26 publications

References 38 publications

Study on the differential proteomics of rat hippocampal mitochondria during deep hypothermic circulatory arrest

Study on the differential proteomics of rat hippocampal mitochondria during deep hypothermic circulatory arrest

Basement membrane proteins as a substrate for efficient Trypanosoma brucei differentiation in vitro

Composite of Peptide‐Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio‐Inspired Soft Material

Contact Info

Product

Resources

About