Ten Years of Laser-Assisted Gametes and Embryo Manipulations

Tadir, Yona

doi:10.1159/000062793

Cited by 4 publications

(8 citation statements)

References 30 publications

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“…Laser-assisted zona drilling was first reported in 1991 by two groups led by Tadir [53] and Palankar [54]. Laser presents an ideal tool for microsurgical procedures, as the energy is easily focussed on the targeted area producing a controlled and precise hole consistent between operators.…”

Section: Laser-assisted Hatchingmentioning

confidence: 99%

“…Initially, non contact mode was adopted for gamete micromanipulation using various laser wavelengths. Subsequently, the contact approach was used with ultraviolet (UV) wavelength delivered by glass pipette, or infrared (IR) wavelength delivered with a quartz fibre [53]. However, the technical advantages of the non-contact mode and the potential of UV radiation to cause harmful mutagenic effects have led to the general preference for non-contact mode using the IR 1.48 μm (1,480 nm) diode laser [55].…”

Section: Laser-assisted Hatchingmentioning

confidence: 99%

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Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

105

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The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards.

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Section: Laser-assisted Hatchingmentioning

confidence: 99%

Section: Laser-assisted Hatchingmentioning

confidence: 99%

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

105

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“…chemical zona drilling with acidic medium 20 ; chemical zona thinning 33 ; laser-assisted hatching 34 and, more recently, piezo technology 35 . The mechanical and the chemical methods require extensive technical skill to produce uniform, well controlled and standardised micro holes using micropipettes mounted on micromanipulators.…”

Section: Techniques Of Assisted Hatchingmentioning

confidence: 99%

“…Laser-assisted zona drilling was first reported in 1991 by two groups led by Tadir 34 and Palankar 40 . Laser presents an ideal tool for microsurgical procedures, as the energy is easily focussed on the targeted area producing a controlled and precise hole consistent between operators.…”

Section: Laser-assisted Hatchingmentioning

confidence: 99%

“…The laser beam may either be directed using an optical lens tangential to the embryo through the zona pellucida in a non-contact mode or the laser may be guided through an optical fibre touching the embryo in a contact mode. Initially, noncontact mode was adopted for gamete micromanipulation using various laser wavelengths 34 . Subsequently, the contact approach was used with ultraviolet (UV) wavelength delivered by glass pipette, or infrared (IR) wavelength delivered with a quartz fibre 34 .…”

Section: Laser-assisted Hatchingmentioning

confidence: 99%

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Assisted hatching in assisted reproduction

Al-Nuaim

2002

BJOG: An International Journal of Obstetrics and Gynaecology

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Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

Ilina

Khramova

Ivanova

et al. 2020

J Assist Reprod Genet

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Purpose To study whether the application of femtosecond laser pulses for zona pellucida (ZP) drilling of blastocysts at the embryonic or abembryonic poles can promote hatching to start immediately through the hole formed and ensure high hatching rates and embryo viability. Methods Mouse blastocyst (E3.5) ZP were microdissected with femtosecond laser pulses (514-nm wavelength, 280-fs pulse duration, 2.5-kHz repetition rate) close to the trophoblast or inner cell mass (ICM). The sizes of the holes formed were in the range of 4.5-8.5 μm. Additional longitudinal incisions (5-7-μm long) on either side of the hole were created to determine whether hatching had started at the correct position. Embryos post-laser-assisted ZP drilling and intact embryos were cultured under standard conditions for 2 days; embryo quality was assessed twice daily. The hatching rates and in vitro and in vivo implantation rates (only for embryos with ZP dissected close to the ICM) were estimated. Results Femtosecond laser-assisted ZP drilling at the early blastocyst stage facilitated embryo hatching to start at the artificial opening with probability approaching 100%. Despite the artificial opening's small size, no embryo trapping during hatching was observed. Both experimental groups had higher hatching rates than the control groups (93.3-94.7% vs. 83.3-85.7%, respectively). The in vitro implantation rate was comparable with that of the control group (92.3% vs. 95.4%). No statistically significant differences were obtained in the in vivo implantation rates between the experimental and control groups. Conclusions Blastocyst-stage femtosecond laser microsurgery of ZP is fast and delicate and enables the hatching process to be initiated in a controlled manner through a relatively small opening, with no embryo trapping.

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Ten Years of Laser-Assisted Gametes and Embryo Manipulations

Cited by 4 publications

References 30 publications

Assisted hatching in assisted reproduction: a state of the art

Assisted hatching in assisted reproduction: a state of the art

Assisted hatching in assisted reproduction

Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

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