Ten-dimensional hyphenation including simulated static gastro-intestinal digestion on the adsorbent surface, planar assays, and bioactivity evaluation for meal replacement products

Abstract

Meal replacement products are normally consumed in weight loss interventions and the treatment of obesity and diabetes. Changing lifestyles and eating habits rendered meal replacement products in the forms of...

Bioactive profiles of edible vegetable oils determined using 10D hyphenated comprehensive high-performance thin-layer chromatography (HPTLC×HPTLC) with on-surface metabolism (nanoGIT) and planar bioassays

“…If co-eluted with C18:2 as in the standard track, this enhancing effect was weakened since the antibacterial effect of C18:2 was stronger ( Figure 7 , zone c ). Compared to previous bioautograms on HPTLC plates silica gel 60 ( 39 , 46 ), C16:0 showed no metabolism-enhancing effect on RP-18 W plates, which was explained by the doubled amount (10 μg/band vs. 5 μg/band) since such enhancing effects are dose-dependent and, in addition, also time-dependent (bioluminescence images monitored for 30 min). The antibacterial response for C8:0–12:0 was very intense against both Gram-negative A. fischeri and Gram-positive B .…”

“…As mentioned for the separation of acylglycerols, polar impurities of the pancreatin co-eluting with the FAs hindered their assignment and could also lead to false-positive interpretations. Using automated heart-cut elution of the interesting zones to RP-HPLC–DAD–HESI-HRMS/MS ( 39 ), these impurities were assigned as the bile acids ursodeoxycholic acid (UDCA), hyodeoxycholic acid (HDCA), (cheno)deoxycholic acid (CDCA/DCA), and cholenic acid ( Figure 4 and Supplementary Table S2 ). The isomers UDCA, HDCA, and (C)DCA were identified in the negative ionization mode (HESI − ) in two separate peaks at retention times of 8.11 min and 8.45 min with [M–H] − at m/z 391.2858 and 391.2860, respectively.…”

“…A retardation shift was observed between reference standards ( Figure 6A ) and samples ( Figure 6B ). Using automated heart cuts to RP-HPLC–DAD–HESI-HRMS/MS ( 39 ), the highest eluting FAs ( Figure 6B , zone d ) were identified as C18:3, C18:2, C16:0, and C18:1 ( Supplementary Figure S9 , zone d , Supplementary Table S3 ). The most intense signal for this zone was from C18:3.…”

“…After the bioassay, HPTLC–UV/Vis/FLD–bioassay–heart cut–RP-HPLC–DAD–HESI-HRMS/MS ( 39 ) equipped with an autoTLC interface ( 40 ) was used to analyze zones of interest directly from the bioautogram. HRMS/MS signals were recorded via the polarity switching full-scan data-dependent MS2 (ddMS2) mode.…”

Simple performance of the planar SOS-Umu-C–FLD genotoxicity bioassay shown for perfume and packaging material analysis

“…The most prominent compounds can straightforwardly be characterized by reagent sequences on the same bioautogram [4,5] and further by direct elution to high-resolution mass spectrometry [6]. Although this is often overlooked in the field of liquid chromatographic hyphenations [7,8], HPTLC provides comparatively much more information about the sample from a single analysis, reported as up to 12D hyphenation [9][10][11], and is more sustainable in terms of plastic material and solvent consumption [12] than effect-directed detection via cuvette or microtiter plate assays associated with column-based separation methods. It is also comparatively much faster and less laborious, given the fact that multiple information is obtained on the same plate, i.e.…”