Temporospatial gene expression and protein localization of matrix metalloproteinases and their inhibitors during mouse molar tooth development

Yoshiba, Nagako; Yoshiba, Kunihiko; Perrin-Schmitt, Fabienne; Cam, Y.; Ruch, J V; Lesot, Hervé

doi:10.1002/dvdy.10352

Cited by 38 publications

(58 citation statements)

References 29 publications

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“…5, third column). The alligator dl MMP14 expression pattern in the present study is similar to that seen in mouse molar development (Yoshiba et al 2003). MMP20 is faintly expressed in bud-stage and cap-stage samples.…”

Section: Sish Of Candidate Moleculessupporting

confidence: 85%

The Molecular Circuit Regulating Tooth Development in Crocodilians

et al. 2016

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Alligators have robust regenerative potential for tooth renewal. In contrast, extant mammals can either renew their teeth once (diphyodont dentition, as found in humans) or not at all (monophyodont dentition, present in mice). Previously, the authors used multiple mitotic labeling to map putative stem cells in alligator dental laminae, which contain quiescent odontogenic progenitors. The authors demonstrated that alligator tooth cycle initiation is related to β-catenin/Wnt pathway activity in the dental lamina bulge. However, the molecular circuitry underlying the developmental progression of polyphyodont teeth remains elusive. Here, the authors used transcriptomic analyses to examine the additional molecular pathways related to the process of alligator tooth development. The authors collected juvenile alligator dental laminae at different developmental stages and performed RNA-seq. This data shows that Wnt, bone morphogenetic protein (BMP), and fibroblast growth factor (FGF) pathways are activated at the transition from pre-initiation stage (bud) to initiation stage (cap). Intriguingly, the activation of Wnt ligands, receptors and co-activators accompanies the inactivation of Wnt antagonists. In addition, the authors identified the molecular circuitry at different stages of tooth development. The authors conclude that multiple pathways are associated with specific stages of tooth development in the alligator. This data shows that Wnt pathway activation may play the most important role in the initiation of tooth development. This result may offer insight into ways to modulate the genetic controls involved in mammalian tooth renewal.

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Section: Sish Of Candidate Moleculessupporting

confidence: 85%

The Molecular Circuit Regulating Tooth Development in Crocodilians

et al. 2016

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“…We assume that MMPs and TIMPs are highly expressed in two key moments that modify caries susceptibility. First, these genes participate in enamel formation, in histomorphogenesis and cytodifferentiation [Yoshiba et al, 2003]. Both events are accompanied by changes in cellular organization and remodeling of the ECM [Yoshiba et al, 2003].…”

Section: Discussionmentioning

confidence: 99%

“…MMP13 is involved in bone development and repair [Yamagiwa et al, 1999;Inada et al, 2004] and is expressed in pulp dental tissue [Sulkala et al, 2004]. TIMP2 was detected in enamel matrix [Yoshiba et al, 2003] and is coexpressed with MMP2 and MMP9 during mouse tooth morphogenesis [Sahlberg et al, 1999]. TIMP2 is also an endogenous inhibitor of MMP13 [Knauper et al, 1996].…”

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confidence: 99%

MMP13 Polymorphism Decreases Risk for Dental Caries

Tannure

Küchler²,

Falagan-Lotsch

et al. 2012

Caries Res

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Recent evidence suggests that genetic studies may contribute to a better understanding of individual susceptibility to caries. Matrix metalloproteinases (MMPs) and their tissue inhibitors have been suggested to be involved in the caries process. The purpose of this study was to determine if polymorphisms in MMP2 (rs243865), MMP9 (rs17576), MMP13 (rs2252070), and TIMP2 (rs7501477) were associated with caries. Eligible unrelated children and adolescents were evaluated using a cross-sectional design. Data on oral health habits was obtained through a questionnaire and caries data was collected by clinical examination. Genotyping of the selected polymorphisms was carried out by real-time PCR. Allele and genotype frequencies were compared between individuals with and without caries experience. Of 505 subjects, 212 were caries-free and most subjects (61.2%) had mixed dentition. Allele frequency of MMP2, MMP13 and TIMP2 was different between caries-affected and caries-free individuals, with significant association for MMP13 (p = 0.004). Mutant allele carriers for MMP13 demonstrated a significantly decreased risk for caries (OR = 0.538, 95% CI 0.313–0.926); this result remained significant after adjustment for candidate genes, type of dentition and dietary factors. Allelic and genotype frequencies of the polymorphism in MMP9 were similar in caries-affected and caries-free individuals. Genetic variations in MMP13 may contribute to individual differences in caries susceptibility. Our findings reinforce that susceptibility to caries results from gene-environment interactions.

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“…47,50 Type 4 collagen is present in the dental basement membrane during the bud to cap stage of tooth development but disappears at later stages because of the MMP action 51,52 ; one of these proteinases is gelatinase A (MMP-2), whose expression has been correlated with collagen 4 and extracellular matrix degradation during the early stages of tooth development and specifically when epithelial cells migrate into the underlying mesenchyme, inducing and maintaining cell polarity and differentiation. 49,53,54 In our experiments, type 4 collagen was expressed only in the inner half of the intermediate layer and seemed to demarcate the future dentin enamel junction. At the same time, MMP-2, which is involved in type 4 collagen degradation, is coexpressed with type 4 collagen, correlating with the presence of a basement membrane under rearrangement, which modulates dental morphogenesis.…”

Section: Discussionmentioning

confidence: 45%

“…At the same time, MMP-2, which is involved in type 4 collagen degradation, is coexpressed with type 4 collagen, correlating with the presence of a basement membrane under rearrangement, which modulates dental morphogenesis. 54 Ameloblastic and odontoblastic markers are highly expressed in the intermediate inner layers.…”

Section: Discussionmentioning

confidence: 99%

Adipose Tissue-Derived Stem Cell in Vitro Differentiation in a Three-Dimensional Dental Bud Structure

Ferro

Spelat

Falini

et al. 2011

The American Journal of Pathology

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Tooth morphogenesis requires sequential and reciprocal interactions between the cranial neural crest-derived mesenchymal cells and the stomadial epithelium, which regulate tooth morphogenesis and differentiation. We show how mesenchyme-derived single stem cell populations can be induced to transdifferentiate in vitro in a structure similar to a dental bud. The presence of stem cells in the adipose tissue has been previously reported. We incubated primary cultures of human adipose tissue-derived stem cells in a dental-inducing medium and cultured the aggregates in three-dimensional conditions. Four weeks later, cells formed a three-dimensional organized structure similar to a dental bud. Expression of dental tissue-related markers was tested assaying lineagespecific mRNA and proteins by RT-PCR, immunoblot, IHC, and physical-chemical analysis. In the induction medium, cells were positive for ameloblastic and odontoblastic markers as both mRNAs and proteins. Also, cells expressed epithelial, mesenchymal, and basement membrane markers with a positional relationship similar to the physiologic dental morphogenesis. Physical-chemical analysis revealed 200-nm and 50-nm oriented hydroxyapatite crystals as displayed in vivo by enamel and dentin, respectively. In conclusion, we show that adipose tissue-derived stem cells in vitro can transdifferentiate to produce a specific three-dimensional organization and phenotype resembling a dental bud even in the absence of structural matrix or scaffold to guide the developmental process.

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Temporospatial gene expression and protein localization of matrix metalloproteinases and their inhibitors during mouse molar tooth development

Cited by 38 publications

References 29 publications

The Molecular Circuit Regulating Tooth Development in Crocodilians

The Molecular Circuit Regulating Tooth Development in Crocodilians

<b><i>MMP13</i></b> Polymorphism Decreases Risk for Dental Caries

Adipose Tissue-Derived Stem Cell in Vitro Differentiation in a Three-Dimensional Dental Bud Structure

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