Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality

Godoy, Sebastián; Tapia, Eduardo; Seit, Paula; Andrade, D. O. de; Sanchez, Evelyn Aparecida Mecenero; Andrade, Paola; Almeida, Aparecida Marques de; Prieto, Humberto

doi:10.1007/s11627-017-9856-z

Cited by 18 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rooting percentages were also much lower (27%) than those obtained in semi-solid medium (50-70%). In Quercus robur (Gatti et al 2017) and in Prunus avium (Godoy et al 2017) carried out the rooting in semi-solid media due to the poor results obtained in TIS.…”

Section: Shoot Numbermentioning

confidence: 99%

“…(McAlister et al 2005), Phoenix dactylifera (Nayyef et al 2019), Pistacia vera (Akdemir et al 2014), Quercus robur (Gatti et al 2017), Q. suber (Pérez et al 2013), Castanea spp. (Vidal et al 2015;Cuenca et al 2017), Prunus avium (Godoy et al 2017), P. cerasifera (Nasri et al 2019), Tectona grandis (Quiala et al 2012), Salix viminalis (Regueira et al 2018), Olea europaea (Benelli and De Carlo 2018), Citrus × latifolia (Bulbarela-Marini et al 2019) among others.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Temporary immersion systems to improve alder micropropagation

José

Blázquez

Cernadas

et al. 2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

A protocol has been developed for the propagation of Alnus glutinosa axillary shoots in liquid medium. The explants were cultured in Woody Plant Medium supplemented with 0.1 mg l − 1 benzyladenine and 0.5 mg l − 1 indole acetic acid. The effect of the bioreactor type (RITA® and Plantform™), frequency of the immersions, regulator concentrations, and volume of medium per explant was investigated. All the treatments with the temporal immersion systems (TIS) increased the proliferation rates. The best results were obtained on using the Plantform™ vessels and, unlike the shoots cultured in RITA® vessels, the culture in Plantform™ had very low hyperhydricity percentages. Shoots originating from the culture in semisolid medium and in Plantform™ rooted in semi-solid medium with 0.1 mg/l indole butyric acid for 7 days. No significant differences were observed in the rooting or acclimatization percentages, with survival percentages greater than 85% being achieved. This is the first work on the use of TIS systems in alder propagation, with the results of this study providing new perspectives for its mass propagation. Key messageA new protocol is described in order to reduce the propagation costs of alder.

show abstract

Section: Shoot Numbermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Temporary immersion systems to improve alder micropropagation

José

Blázquez

Cernadas

et al. 2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Advances in in vitro culture techniques such as TIS have recently been developed to improve mass propagation efficiency that was difficult to accomplish using conventional propagation systems such as SSS or CIS [17]. TIS mass propagation success has previously been reported for various plant species [18,19]-including orchids [11,20,21].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Kunakhonnuruk

Inthima

Kongbangkerd

2019

Biology

View full text Add to dashboard Cite

Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods. Temporary immersion system (TIS) has been successfully applied for large-scale propagation in various plants species. Propagation efficiency of E. flava using TIS was investigated and compared with conventional semi-solid system (SSS) and liquid continuous immersion system (CIS). The highest percentage of new shoot and shoot bud formation was obtained from TIS, followed by CIS and SSS, respectively. Growth parameters as indicated by number of new shoots, shoot buds, shoot height and leaves per explant were significantly higher using TIS than with SSS and CIS. Moreover, the maximum number of new shoots and shoot buds per replication were reliably obtained from TIS higher than SSS and CIS. After acclimatization, the highest survival percentage of plantlets was observed in TIS (76.7%), with 60% surviving after eight weeks of transplantation in artificial stream. TIS was determined as the most suitable culture system for in vitro mass propagation of E. flava compared to CIS and SSS.

show abstract

“…In other plants, shoots obtained by TIS were rooted ex vitro, as reported for chestnut and teak , or in vitro by using SS, as reported for pistachio , cherry , and oak . Except for oak shoots, the use of TIS increased the number and quality of shoots destined to rooting, improving the overall process with regard to conventional micropropagation.…”

Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 91%

“…For several years, the use of bioreactors has become part of the daily routine in most plant tissue culture laboratories. These systems can improve proliferation, rooting, plant conversion and acclimation of a wide range of plants, including trees The industrial applications of bioreactors are widespread, and besides the references in which participation of companies is cited , many companies culturing woody plants use bioreactors at experimental or commercial levels. Figure shows some examples of the propagation of woody plants in bioreactors on an industrial scale.…”

Section: Advantages Disadvantages and Prospects Of The Use Of Biorementioning

confidence: 99%

Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

View full text Add to dashboard Cite

Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

show abstract

Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality

Cited by 18 publications

References 37 publications

Temporary immersion systems to improve alder micropropagation

Temporary immersion systems to improve alder micropropagation

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Use of bioreactor systems in the propagation of forest trees

Contact Info

Product

Resources

About