Temporal variation of diazotrophic community abundance and structure in surface and subsoil under four fertilization regimes during a wheat growing season

Wang, Jichen; Zhang, Dan; Zhang, Li; Li, Jing; Raza, Waseem; Huang, Qiwei; Shen, Qirong

doi:10.1016/j.agee.2015.09.039

Cited by 60 publications

(47 citation statements)

References 42 publications

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“…These results suggested that the change in diazotrophic structure was likely to have been caused by multiple factors rather than a single soil property. Furthermore, the PCA profile implied that intercropping had a weaker effect on the diazotrophic structure than crop growth stage, which is in line with previous findings [33,44] .…”

Section: Discussionsupporting

confidence: 90%

“…In this study, we combined real-time PCR and Illumina MiSeq sequencing to reveal the effects of cropping system and crop growth stage on the abundance, diversity and structure of the diazotrophic community in oat rhizosphere soil. The nifH gene abundance across the three cropping systems and two stages were found to be much higher than that observed in potato fields [18,32] , wheat fields [33] and sorghum rhizosphere soils [34] . The presence of symbiotic N-fixers in oat [31,35] and intercropped with leguminous crops might be the main reasons for the higher abundance of nifH gene in the soil samples assayed.…”

Section: Discussionmentioning

confidence: 56%

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The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Yang¹,

Feng²,

Hu³

et al. 2019

Front. Agr. Sci. Eng.

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In this study, the abundance, diversity and structure of the diazotrophic community in oat rhizosphere soil in three cropping systems and at two oat growth stages were investigated using real-time PCR and Illumina MiSeq sequencing. The nifH gene abundance in oat-soybean intercropping (OSO) and oat-mungbean intercropping (OMO) was significantly greater than that in sole oat (O), but the nifH gene abundance significantly decreased at the later stage in all the treatments. Alpha diversity indices in OSO and OMO were higher at the heading stage, but lower at the maturity stage than that in O. Bradyrhizobium and Skermanella were the dominant genera identified in all samples, with an average proportion of 35.8% and 12.4%, respectively. The proportion of dominant genera showed significant differences and varied with cropping system and growth stage. Principal component analysis showed that growth stage had a stronger effect than intercropping on the diazotrophic community structure. However, Mantel test and redundancy analysis showed there was no environmental factor significantly correlated to the diazotrophic community structure. Our results demonstrate that intercropping had a weaker effect than growth stage on the abundance, diversity and structure of the diazotrophic community in oat rhizosphere soil.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 56%

The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Yang¹,

Feng²,

Hu³

et al. 2019

Front. Agr. Sci. Eng.

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“…Examination of the nifH gene that encodes the nitrogenase reductase subunit has been used to assess the phylogeny, diversity, and abundance of diazotrophic communities (Zehr et al, 2003). Surveys of nifH sequences in soil have reported important contributions which in turn have increased our understanding about N 2 -fixation potential and dynamics in this environment (Hamelin, Fromin, Tarnawski, Teyssier-Cuvelle, & Aragno, 2002;Niederberger et al, 2012;Rösch, Mergel, & Bothe, 2002;Wang et al, 2016;Yeager et al, 2004). Several studies emphasize that a great part of the diazotrophic diversity corresponds to uncharacterized and uncultured soil bacteria (Duc, Noll, Meier, Bürgmann, & Zeyer, 2009;Gaby & Buckley, 2011;Hsu & Buckley, 2009).…”

mentioning

confidence: 99%

Analysis of nifH‐RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N₂‐fixing community depending on depth and agricultural use of soil

et al. 2017

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In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)‐based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no‐till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no‐till management. Because crop production relies on soil‐borne microorganism's activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land‐use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.

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“…ile NH 4 -N'u içeren gübrelerin kullanılması gerekliliğini doğurmuştur (Çizelge 6). Wang et al (2016) tarafından yapılan bir araştırmada bu sonucu destekler nitelikte olup; araştırıcılar, yüzey toprağının organik madde (OM) ve amonyum içerikleri ile diazotrofik topluluk yapıları aralarında pozitif korelasyon saptamışlardır.…”

Section: Tartişma Mikrobiyolojik Ve Biyokimyasal Sonuçlar Ile Ilgili unclassified

The Effect of Biological Fertilizer Containing N-Fixer on Microbiological and Biochemical Soil Characteristics and Maize Yield under Mediterranean Climatic Conditions

Ayyildiz

Kayikçioğlu

2019

Ege Üniversitesi Ziraat Fakültesi Dergisi

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Bu çalışmada topraklara, havanın serbest azotunu (N 2) bağlama yeteneğine sahip organizmalardan oluşan (Azospirillum sp., Azorhizobium sp. ve Azoarcus sp.) ticari bir preparatın, Akdeniz iklimi etkisi altında bulunan Typic Xerofluvent özellikteki toprakta etkinlik potansiyelleri ile mevcut biyolojik özellikleri üzerine etkileri araştırılmıştır. Materyal ve Metot: Biyolojik gübre uygulamalı ve uygulamasız parsellere (0-0.5 g ha-1 ; BG 0-BG 1), 3 doz kimyasal azotlu gübre (0-125-250 kg N ha-1 ; N 0-N 1-N 2) uygulamalarının, toprağın mikrobiyolojik (mikrobiyal biyokütle karbonu, MBC; toprak solunumu, BSR; azot mineralizasyonu, N min ; genel bakteri sayısı, GB, Azospirillum sp. sayısı, AZO) ve biyokimyasal aktivitesine (dehidrogenaz, DHG; proteaz, PRO, üreaz, UA) ve test bitkisi olarak yetiştirilen mısır bitkisinin (Zea mays var. indentata) verim ve azot içeriğine olan etkileri faktöriyel bazda incelenmiştir. Bulgular: Biyolojik gübre uygulamasıyla topraklarda incelenen mikrobiyolojik ve biyokimyasal parametrelerden MBC, DHG ve AZO parametreleri önemli düzeyde pozitif olarak etkilenirken (P<0.05); BSR, N min ve GB parametrelerinde de artış saptanmış ancak istatistiksel olarak önemli düzeyde bulunmamıştır (P>0.05). Buna karşılık hidrolazlar grubundan olan ve topraklarda N-döngüsünde yer alan iki enzimin aktivitesinde bir azalma saptanmasına karşın bu azalış da istatistiksel olarak önemli bulunmamıştır (P>0.05). NO 3-N ile AZO arasında ortaya çıkan negatif korelasyon (-0.468**), azotlu gübrelemede Azospirillum sp. ile NH 4-N'u içeren gübrelerin kullanılmasının daha uygun olacağını göstermektedir. Biyolojik gübre uygulamalarına bağlı olarak mısır bitkisinin verimi % 24-69 oranında, azot içeriği ise % 3-11 oranında artış göstermiştir. Bu parametreleri en fazla uyaran uygulama ise N 1 BG 1 uygulaması olmuştur. Sonuç: Deneme sonuçları değerlendirildiğinde, hem toprak sağlığının sürdürülebilirliğini ve hem de bitkisel üretimi desteklemek amacıyla 0.5 g ha-1 dozunda biyolojik gübre ile 125 kg N ha-1 azot dozunun birlikte kullanılması önerilmektedir.

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Temporal variation of diazotrophic community abundance and structure in surface and subsoil under four fertilization regimes during a wheat growing season

Cited by 60 publications

References 42 publications

The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Analysis of nifH‐RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N₂‐fixing community depending on depth and agricultural use of soil

The Effect of Biological Fertilizer Containing N-Fixer on Microbiological and Biochemical Soil Characteristics and Maize Yield under Mediterranean Climatic Conditions

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Temporal variation of diazotrophic community abundance and structure in surface and subsoil under four fertilization regimes during a wheat growing season

Cited by 60 publications

References 42 publications

The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Analysis of nifH‐RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2‐fixing community depending on depth and agricultural use of soil

The Effect of Biological Fertilizer Containing N-Fixer on Microbiological and Biochemical Soil Characteristics and Maize Yield under Mediterranean Climatic Conditions

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Analysis of nifH‐RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N₂‐fixing community depending on depth and agricultural use of soil