Temporal variation of antibiotic resistome and pathogens in food waste during short-term storage

Lactobacillus paracasei ET-22 and derived postbiotics reduce halitosis and modulate oral microbiome dysregulation – a randomized, double-blind placebo-controlled clinical trial

“…21 DNA quality was assessed using agarose gel and a NanoDrop2000 spectrophotometer. The V3–V4 variable region of 16S rRNA gene sequences was amplified with the 338F and 806R PCR primer 22 and sequenced on the Illumina MiSeq platform at Majorbio (Shanghai, China). Sequencing library preparation was performed using the NEXTflex TM Rapid DNA-Seq Kit (Bioo Scientific, USA).…”

Wastewater surveillance of antibiotic resistant bacteria for public health action: Potential and Challenges

“…Focusing wastewater surveillance on ARGs instead of ARBs also is problematic. ARGs are detectable in food (21,22), food animals, and in the biofilms found in sinks and pipes (23) [Figure 1]. ARGs can be transmitted between bacteria of the same or different species by bacterial pathogens (phage) or via horizontal gene transfer.…”

Pathogenic Bacteria Are the Primary Determinants Shaping PM2.5-Borne Resistomes in the Municipal Food Waste Treatment System

“…As recently highlighted, ARGs and pathogens (e.g., Staphylococcus aureus, Pseudomonas aeruginosa) can rapidly propagate during FW spoilage and induce an epidemic of airborne AMR. 26,27 Unlike waste treatment environments characterized by diverse and abundant antibiotics, the FW is not typically heavily polluted by anthropogenic antibiotics. Thus, the high prevalence of AMR in FW is probably driven more by their complex microbial ecology, which includes microbial competition (i.e., stress gradient hypothesis), proliferation of ARG hosts, and horizontal gene transfer (HGT).…”

“…However, the FW recycling process often leads to severe bioaerosol pollution due to sorting, mechanical agitation, aeration, and biosolid/liquid aerosolization. , Airborne culturable microorganism levels in FW composting plants have been reported to be as high as 10 4 –10 5 cfu/m 3 , with the potential to spread over hundreds of meters and persist for days to weeks. − More importantly, FW has been identified as a significant ARG reservoir, − among which ARGs and related resistant bacteria would be inevitably released into ambient air via bioaerosols and further inhaled by nearby humans, especially in densely populated megacities. As recently highlighted, ARGs and pathogens (e.g., Staphylococcus aureus, Pseudomonas aeruginosa ) can rapidly propagate during FW spoilage and induce an epidemic of airborne AMR. , …”

“…Unlike waste treatment environments characterized by diverse and abundant antibiotics, the FW is not typically heavily polluted by anthropogenic antibiotics. Thus, the high prevalence of AMR in FW is probably driven more by their complex microbial ecology, which includes microbial competition (i.e., stress gradient hypothesis), proliferation of ARG hosts, and horizontal gene transfer (HGT). ,, These microbial processes may directly alter the airborne resistome within FW environments. Besides, specific biochemical properties of FW (e.g., high salt and oil content, and low pH) may have distinct effects on AMR development compared to other waste settings, , particularly because such properties also potentially influence the chemical composition of bioaerosols, further shaping the fate of airborne resistome.…”