Temporal variability of cyanobacterial populations in the water and sediment samples of Lake Taihu as determined by DGGE and real-time PCR

Diversity and dynamics of picocyanobacteria and the bloom-forming cyanobacteria in a large shallow eutrophic lake (lake Chaohu, China)

“…The low biomass and small size of these habitats compared with other bloom-forming cyanobacteria in eutrophic water bodies have excluded picocyanobacteria in former studies based on light microscopy (Ouellette et al, 2006). Molecular methods show that picocyanobacteria are abundant in eutrophic lakes (Kolmonen et al, 2004;Ouellette et al, 2006;Ye et al, 2011). Molecular methods are Diversity and dynamics of picocyanobacteria and bloom-forming cyanobacteria in a large shallow eutrophic lake (lake Chaohu, China) N o n c o m m e r c i a l u s e o n l y useful for detecting and classifying cyanobacteria, especially picocyanobacteria, because they lack sufficient reliable morphologic features (Raven, 1998).…”

Evaluation of DNA extraction methods for freshwater eukaryotic microalgae

“…In contrast, the study of microalgae within natural and engineered freshwater systems using molecular biology techniques is in its infancy. A few studies have been carried out on photoautotrophic picoplankton communities from lakes using fluorescence in situ hybridisation (FISH) (Lepere et al, 2010) and clone libraries, based on the 18S rRNA gene (See et al, 2005;Lefranc et al, 2005), though the majority of freshwater studies focus on cyanobacteria (Zwart et al, 2005;Ye et al, 2011). Very few studies have used molecular approaches to study microalgal communities in wastewater treatment.…”

Gel Electrophoresis Based Genetic Fingerprinting Techniques on Environmental Ecology

“…In another study, the authors showed that the different bacterial and archaeal profiles within the highly polluted anoxic marine sediments in the different locations from the Marmara Sea (Cetecioglu et al, 2009). Ye et al (2011) showed the temporal variability of cyanobacteria in the water and sediment of a lake.…”