Temporal Transcription Program of Recombinant
            <i>Autographa californica</i>
            Multiple Nucleopolyhedrosis Virus

Jiang, Shih Sheng; Chang, I–Shou; Huang, Lin-Wei; Chen, Po‐Cheng; Wen, Chi‐Chung; Liu, Shu-Chen; Chien, Li‐Chu; Lin, Chung‐Yen; Hsiung, Chao A.; Juang, Jyh‐Lyh

doi:10.1128/jvi.01158-06

Cited by 39 publications

(46 citation statements)

References 55 publications

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“…1). This transcription profile is consistent with the findings of Jiang et al (26), who examined the temporal transcription patterns of AcMNPV through a microarray. We used a repair virus containing an HA tag at the Ac34 N terminus to investigate the expression pattern and localization of Ac34.…”

Section: Discussionsupporting

confidence: 92%

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An ac34 Deletion Mutant of Autographa californica Nucleopolyhedrovirus Exhibits Delayed Late Gene Expression and a Lack of Virulence In Vivo

Cai

Zhao

Qiu

et al. 2012

J Virol

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Ac34 and its homologs are highly conserved in all sequenced alphabaculoviruses. In this paper, we show that ac34 transcripts were detected from 6 to 48 h postinfection (p.i.) in Autographa californica nucleopolyhedrovirus (AcMNPV)-infected Sf9 cells. Ac34 localized to both the cytoplasm and the nuclei of infected cells but was not a viral structural protein. To determine the function of ac34 in the viral life cycle, an ac34 knockout AcMNPV (vAc34KO) was constructed. Compared with wild-type and repair viruses, vAc34KO exhibited an approximately 100-fold reduction in infectious virus production. Further investigations showed that the ac34 deletion did not affect the replication of viral DNA, polyhedron formation, or nucleocapsid assembly but delayed the expression of late genes, such as vp39 , 38k , and p6.9 . Bioassays revealed that vAc34KO was unable to establish a fatal infection in Trichoplusia ni larvae via per os inoculation. Few infectious progeny viruses were detected in the hemolymph of the infected larvae, indicating that the replication of vAc34KO was attenuated. These results suggest that Ac34 is an activator protein that promotes late gene expression and is essential for the pathogenicity of AcMNPV.

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Section: Discussionsupporting

confidence: 92%

“…In a microarray assay, ac34 was grouped in the ac31-to-ac35 cluster of genes with synchronized expression patterns. Its transcription began in the early phase, and maximum transcription levels were observed in the late phase of infection (26).…”

mentioning

confidence: 99%

An ac34 Deletion Mutant of Autographa californica Nucleopolyhedrovirus Exhibits Delayed Late Gene Expression and a Lack of Virulence In Vivo

Cai

Zhao

Qiu

et al. 2012

J Virol

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“…One possible explanation for the production of a longer cry4Ba fragment is that the transcribed cry4Ba mRNA may have been elongated until reaching the ORF 603 stop codon ( Figure 1A). Similar interpretations for baculovirus transcriptional profiles have been reported in previous works, in which the overlapping nature of viral mRNA was found to be a common event (Jiang et al, 2006;Smith & Juang, 2007;Yamagish et al, 2007). The RT-PCR analysis confirmed the exogenous genes transcription in the very late phase of infection.…”

Section: Transcriptional Analysis Of Cry4aa and Cry4ba Genes In Insecsupporting

confidence: 89%

Cry4aa and Cry4ba From Bacillus Thuringiensis Subsp. Israelensis Expressed in Insect Cells by Recombinant Baculoviruses Are Toxic to Aedes Aegypti Larvae

Corrêa¹,

Aguiar²,

Monnerat³

et al. 2013

VR&R

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Bacillus thuringiensis subsp. israelensis (Bti) is highly toxic to mosquito larvae. Most of its toxicity relies on δ-endotoxins (Cry and Cyt) crystalline inclusions produced at the sporulation phase of growth. Obtention of individual Bti mosquitocidal toxins is crucial for the understanding of Bti´s potential activity against dipteran larvae. A Cry toxin expression system based on baculovirus was developed to produce two of the Cry toxins comprised in the crystals of Bti. Th e cry4Aa and cry4Ba genes from two Brazilian strains of Bacillus thuringiensis were amplifi ed by PCR, cloned into a plasmid cloning vector and sequenced. Sequence analysis of the cry4Aa and cry4Ba genes showed high identity to previous known cry genes. Both cry4Aa and cry4Ba genes were further cloned into transfer vectors for construction of recombinant baculoviruses.. Aft er isolation of the recombinant viruses, they were used to infect insect cells (BTI-TN5B1-4) that were analyzed by light microscopy at diff erent times post infection. Putative crystals consisting of either Cry4Aa or Cry4Ba were observed in the cytoplasm of infected insect cells. RT-PCR was performed with mRNA from insect cell extracts (72 h.p.i.) in order to confi rm the presence of the genes specifi c transcripts. Recombinant virus-infected insect extracts (120 h.p.i.) were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE) showing the presence of polypeptide bands of around 128 and 130 kDa, corresponding, respectively to the sizes of the proteins Cry4Aa and Cry4Ba. Bioassays with virus-infected insect extracts were shown to be toxic to second instar Aedes aegypti larvae, confi rming the usefulness of this expression system for the study of Cry proteins.

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“…Several prior studies of AcMNPV transcriptomes have been performed with microarrays and by real-time PCR analysis, although data are often difficult to interpret in light of the inherent problems associated with the tightly packed AcMNPV genome and overlapping transcripts (31)(32)(33). Recently, the Bombyx mori NPV (BmNPV) transcriptome was examined using RNA-Seq, and Illumina reads were mapped to a reference transcriptome database (34).…”

mentioning

confidence: 99%

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

Chen

Zhong

Fei

et al. 2013

J Virol

164

225

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Temporal Transcription Program of Recombinant Autographa californica Multiple Nucleopolyhedrosis Virus

Cited by 39 publications

References 55 publications

An ac34 Deletion Mutant of Autographa californica Nucleopolyhedrovirus Exhibits Delayed Late Gene Expression and a Lack of Virulence In Vivo

An ac34 Deletion Mutant of Autographa californica Nucleopolyhedrovirus Exhibits Delayed Late Gene Expression and a Lack of Virulence In Vivo

Cry4aa and Cry4ba From Bacillus Thuringiensis Subsp. Israelensis Expressed in Insect Cells by Recombinant Baculoviruses Are Toxic to Aedes Aegypti Larvae

The Transcriptome of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Cells

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