Temporal-Specific Interaction of NF-YC and CURLY LEAF during the Floral Transition Regulates Flowering

Liu, Xu; Yang, Yuhua; Hu, Yilong; Zhou, Limeng; Yuge, Louis; Hou, Xue‐Long

doi:10.1104/pp.18.00296

Cited by 43 publications

(29 citation statements)

References 48 publications

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“…This is likely the case, as we and others have shown that SVP represses flowering in a dosage-sensitive manner (Hartmann et al, 2000;Li et al, 2015). Of note, we and others have consistently found the up-regulation of FT in the clf-29 mutants (Farrona et al, 2011;Jiang et al, 2008;Liu et al, 2018). But, unlike the previous studies, we did not find significant CLF/SWN enrichment at this locus (Supporting Information Figure S10b).…”

Section: Discussioncontrasting

confidence: 64%

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

et al. 2019

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The Polycomb Group (PcG) proteins form two protein complexes, PcG Repressive Complex 1 ( PRC 1) and PRC 2, which are key epigenetic regulators in eukaryotes. PRC 2 represses gene expression by catalyzing the trimethylation of histone H3 lysine 27 (H3K27me3). In Arabidopsis ( Arabidopsis thaliana ), CURLY LEAF ( CLF ) and SWINGER ( SWN ) are two major H3K27 methyltransferases and core components of PRC 2, playing essential roles in plant growth and development. Despite their importance, genome‐wide binding profiles of CLF and SWN have not been determined and compared yet. In this study, we generated transgenic lines expressing GFP ‐tagged CLF / SWN under their respective native promoters and used them for Ch IP ‐seq analyses to profile the genome‐wide distributions of CLF and SWN in Arabidopsis seedlings. We also profiled and compared the global H3K27me3 levels in wild‐type ( WT ) and PcG mutants ( clf , swn , and clf swn ). Our data show that CLF and SWN bind to almost the same set of genes, except that SWN has a few hundred more targets. Two short DNA sequences, the GAGA ‐like and Telo ‐box‐like motifs, were found enriched in the CLF and SWN binding regions. The H3K27me3 levels in clf , but not in swn , were markedly reduced compared with WT ; and the mark was undetectable in the clf swn double mutant. Further, we profiled the transcriptomes in clf , swn , and clf swn, and compared that with WT . Thus this work provides a useful resource for the plant epigenetics community for dissecting the functions of PRC 2 in plant growth and development.

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Section: Discussioncontrasting

confidence: 64%

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

et al. 2019

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“…Moreover, the Arabidopsis ABF proteins interact with each other (Yoshida et al, ). Specifically, Arabidopsis ABF3 and ABF4 regulate SOC1 expression, thereby affecting drought stress responses via interactions with NF‐YC (Hwang, Susila, Nasim, Jung, & Ahn, ), which is a key component that integrates GA and ABA signaling and regulates seed germination and flowering (Liu et al, ; Liu et al, ). In pear, ABF3 may function with other components to regulate the expression of downstream genes associated with dormancy transitions and other stress responses.…”

Section: Discussionmentioning

confidence: 99%

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

Yang

et al. 2020

Plant Cell & Environment

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Bud dormancy is indispensable for the survival of perennial plants in cold winters.Abscisic acid (ABA) has essential functions influencing the endo-dormancy status. Dormancy-associated MADS-box/SHORT VEGETATIVE PHASE-like genes function downstream of the ABA signalling pathway to regulate bud dormancy. However, the regulation of DAM/SVP expression remains largely uncharacterized. In this study, we confirmed that endo-dormancy maintenance and PpyDAM3 expression are controlled by the ABA content in pear (Pyrus pyrifolia) buds. The expression of pear ABRE-BINDING FACTOR3 (PpyABF3) was positively correlated with PpyDAM3 expression. Furthermore, PpyABF3 directly bound to the second ABRE in the PpyDAM3 promoter to activate its expression. Interestingly, both PpyABF3 and PpyDAM3 repressed the cell division and growth of transgenic pear calli. Another ABA-induced ABF protein, PpyABF2, physically interacted with PpyABF3 and disrupted the activation of the PpyDAM3 promoter by PpyABF3, indicating DAM expression was precisely controlled. Additionally, our results suggested that the differences in the PpyDAM3 promoter in two pear cultivars might be responsible for the diversity in the chilling requirements. In summary, our data clarify the finely tuned regulatory mechanism underlying the effect of ABA on DAM gene expression and provide new insights into ABA-related bud dormancy regulation. K E Y W O R D S ABF3, Abscisic acid, bud dormancy, DAM, pear

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“…There may be at least three reasons to explain this phenomenon: (i) Epigenetic mechanism. Previous evidence supported that the NF-Y transcription factor as important modulators of epigenetic marks controlling flowering [50][51][52][53] In summary, to elucidate the role of NF-Y transcription factor in poplar flowering induction and molecular regulation mechanism will be important for people to understand the role and function of NF-Y transcription factor family in woody plants, and provide important theoretical basis for regulating flowering time and shortening breeding cycle.…”

Section: Regulation Of Flowering Pathway Genes In the Transgenicmentioning

confidence: 88%

Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants

et al. 2019

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Background: Compared with annual herbaceous plants, woody perennials require a longer period of juvenile phase to flowering, and many traits can be only expressed in adulthood, which seriously makes the breeding efficiency of new varieties slower. For the study of poplar early flowering, the main focus is on the study Arabidopsis homologue gene CO/FT. Based on studies of Arabidopsis, rice and other plant species, some important research progress has been made on the regulation of flowering time by NF-Y subunits. However, little is known about the function of NF-Y regulating flowering in poplar. Results: In the present study, we have identified PtNF-YB family members in poplar and focus on the function of the PtNF-YB1 regulate flowering timing using transgenic Arabidopsis and tomato. To understand this mechanisms, the expression levels of three known flowering genes (CO, FT and SOC1) were examined with RT-PCR in transgenic Arabidopsis. We used the Y2H and BiFC to assay the interactions between PtNF-YB1 and PtCO (PtCO1 and PtCO2) proteins. Finally, the potential molecular mechanism model in which PtNF-YB1 play a role in regulating flowering in poplar was discussed. Conclusions: In this study, we have characterized the poplar NF-YB gene family and confirmed the function of the PtNF-YB1 regulate flowering timing. At the same time, we found that the function of PtNF-YB1 to improve early flowering can overcome species barriers. Therefore, PtNF-YB1 can be used as a potential candidate gene to improve early flowering by genetic transformation in poplar and other crops.

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Temporal-Specific Interaction of NF-YC and CURLY LEAF during the Floral Transition Regulates Flowering

Cited by 43 publications

References 48 publications

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants

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