Temporal single-cell transcriptomes of zebrafish spinal cord pMN progenitors reveal distinct neuronal and glial progenitor populations

Scott, Kayt; O’Rourke, Rebecca; Winkler, Caitlin C.; Kearns, Christina A.; Appel, Bruce

doi:10.1016/j.ydbio.2021.07.010

Cited by 21 publications

(37 citation statements)

References 83 publications

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“…In addition, the existence of progenitors for radial glia, interneurons, OPCs, and motor neurons is consistent with the finding that multiple cell types can arise from the same clone of olig2 + cells (6,7,38). Similar to recent work (24), we also find pri-OPCs appearing as early as 28 hpf, when motor neurons are actively generated. This seems to support a "segregating" model in which motor neurons and oligodendrocyte precursors segregate early (39).…”

Section: Discussionsupporting

confidence: 92%

“…This subpopulation was observable at 28 hpf and still retained at 60 hpf ( Fig. 2 D ), suggesting a segregating model of OPC and motor neurons, in which glial fate was restricted prior to cessation of motor neuron generation ( 24 ).…”

Section: Resultsmentioning

confidence: 95%

“…Consistently, pseudotime analysis revealed the developmental trajectory from pri-OPCs to OPCs (Figure 2E). This subpopulation was observable at 28 hpf, and still retained at 60 hpf (Figure 2D), suggesting a segregating model of OPC and motor neurons, in which glial fate was restricted prior to cessation of motor neuron generation (24).…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 93%

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Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain

Xing

Chai²,

Wang³

et al. 2022

Journal of Biological Chemistry

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 95%

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 93%

See 1 more Smart Citation

Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain

Xing

Chai²,

Wang³

et al. 2022

Journal of Biological Chemistry

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“…The increased cell death of progenitor cells, especially at an early stage of neurogenesis, can be a contributing factor in reducing progenitor pools leading to compromised neurogenesis. That is the case for oligodendrocyte progenitor populations in zebrafish spinal cord development 49 . In the case of dla+ cells, regulation by MT3 seems to depend on two different mechanisms where thraa and thrab have different roles.…”

Section: Discussionmentioning

confidence: 93%

Maternal thyroid hormone increases neural cell diversity during zebrafish spinal cord neurodevelopment

Silva

Campinho

2022

Preprint

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Maternally derived thyroid hormone (MT3) is a fundamental factor for vertebrate neurodevelopment. In humans, mutations on the T3 exclusive transporter monocarboxylic acid transporter 8 (MCT8) lead to the Allan-Herndon-Dudley syndrome (AHDS). Patients with AHDS present severe underdevelopment of the central nervous system with cognitive and locomotory consequences. Functional impairment of the zebrafish T3 exclusive membrane transporter MCT8 has been shown to phenocopy the symptoms observed in human patients with AHDS, thus providing an outstanding animal model to study this human condition. In this zebrafish model, MT3 acts as an integrator of different key developmental pathways during zebrafish neurodevelopment. Here we expand this knowledge by determining the developmental time of action of MT3 that occurs in very defined temporal intervals during zebrafish neurodevelopment. We have determined that MT3 is not involved in neural specification but is fundamental for developing particular neural progenitors and the consequent neural lineages that originate from them. Our data provide evidence that MT3 achieves this likely by modulation NOTCH signalling in a cell non-autonomous way. The findings show that MT3 expands the cell diversity output of neural progenitor cells, establishing a cellular background behind human AHDS and inherited limited CNS development.

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“…Ascl1 is a basic helix-loop-helix transcription factor that regulates oligodendrocyte production in mice ( Parras et al, 2004 , 2007 ; Vue et al, 2014 ). Zebrafish paralogs Ascl1a and Ascl1b are expressed in the developing neural tube, and Ascl1a mRNA expression has been identified in both neurons and oligodendrocyte lineage cells ( Allende and Weinberg, 1994 ; Scott et al, 2021 ). Whether zebrafish Ascl1a functionally participates in oligodendrogenesis has yet to be determined.…”

Section: Defining the Gnathostome Oligodendrocyte Cell Typementioning

confidence: 99%

Evolutionary Origins of the Oligodendrocyte Cell Type and Adaptive Myelination

Hines

2021

Front. Neurosci.

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Oligodendrocytes are multifunctional central nervous system (CNS) glia that are essential for neural function in gnathostomes. The evolutionary origins and specializations of the oligodendrocyte cell type are among the many remaining mysteries in glial biology and neuroscience. The role of oligodendrocytes as CNS myelinating glia is well established, but recent studies demonstrate that oligodendrocytes also participate in several myelin-independent aspects of CNS development, function, and maintenance. Furthermore, many recent studies have collectively advanced our understanding of myelin plasticity, and it is now clear that experience-dependent adaptations to myelination are an additional form of neural plasticity. These observations beg the questions of when and for which functions the ancestral oligodendrocyte cell type emerged, when primitive oligodendrocytes evolved new functionalities, and the genetic changes responsible for these evolutionary innovations. Here, I review recent findings and propose working models addressing the origins and evolution of the oligodendrocyte cell type and adaptive myelination. The core gene regulatory network (GRN) specifying the oligodendrocyte cell type is also reviewed as a means to probe the existence of oligodendrocytes in basal vertebrates and chordate invertebrates.

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Temporal single-cell transcriptomes of zebrafish spinal cord pMN progenitors reveal distinct neuronal and glial progenitor populations

Cited by 21 publications

References 83 publications

Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain

Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain

Maternal thyroid hormone increases neural cell diversity during zebrafish spinal cord neurodevelopment

Evolutionary Origins of the Oligodendrocyte Cell Type and Adaptive Myelination

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