Temporal regulation of light-induced fos and fos-like protein expression in the ventrolateral subdivision of the rat suprachiasmatic nucleus

Wj, Schwartz; Takeuchi, Junichi; Shannon, William D.; Em, Davis; Aronin, Neil

doi:10.1016/0306-4522(94)90082-5

Cited by 93 publications

(42 citation statements)

References 35 publications

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“…3C; visual estimation only). The levels were more similar to those expected after light exposure 1-2 hr later in the active phase (Schwartz et al, 1994). These results indicate that a phase advance of Ͼ2 hr in the circadian rhythm of SCN sensitivity to light is accomplished within 1 hr after the sleep deprivation procedure.…”

Section: Clock Resetting By Sleep Deprivation Is Rapidsupporting

confidence: 68%

Circadian Clock Resetting by Sleep Deprivation without Exercise in the Syrian Hamster

2000

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Circadian rhythms in several species can be phase-shifted by procedures that stimulate locomotor activity ("exercise") during the usual sleep period. The role of arousal or sleep loss, independent of activity, in this effect has not been adequately resolved. We show here, using the sleep deprivation procedure of gentle handling, that comparably large phase shifts (up to 240 min advances) of the rest-activity cycle can be induced in Syrian hamsters by 3 hr of behavioral arousal, with minimal locomotion, beginning 6 hr before the usual active period. Horizontal distance traveled during the deprivation procedure averaged ϳ0.08 km, compared to 2.5 km typical in exercise studies. Hamsters requiring fewer interventions exhibited larger shifts, suggesting that the level or continuity of spontaneous arousal determines shift size. The circadian rhythm of light-induced c-fos expression in the suprachiasmatic nucleus (SCN) was used as a phase marker to further demonstrate that the clock is reset within 1 hr after a 3 hr deprivation. Sleep deprivation mimicked the effects of exercise on basal c-fos expression in two components of the circadian system, suppressing basal Fos immunoreactivity in the SCN, and increasing Fos in the intergeniculate leaflet. Sleep deprivation without exercise in hamsters can rapidly reset the circadian clock and alter gene expression within the circadian system.Key words: circadian rhythms; nonphotic entrainment; c-fos; wheel running; phase shifts; suprachiasmatic nucleus Forty years ago, it was first suggested that an animal's state of arousal or level of locomotor activity might affect properties of its circadian clock (Aschoff, 1960). Convincing evidence followed some 25 years later, when it was demonstrated that running in an activity wheel can alter the period (Yamada et al., 1986) or shift the phase (Reebs and Mrosovsky, 1989) of circadian rhythms in nocturnal rodents. Since then, it has been shown repeatedly, using a range of arousing stimuli applied during the usual rest phase of the circadian sleep-wake cycle, that substantial phase advance shifts (up to 4 hr) are induced if subjects run during or after the stimulus but usually not if running is absent or prevented (Hastings et al., 1998;. Both the magnitude and the direction of these shifts are gated by circadian phase, with maximal phase advance shifts evident when activity is stimulated near the middle of the rest period and small phase delays (Յ1 hr) when activity is stimulated during the latter half of the usual wake phase of the circadian cycle (Bobrzynska and Mrosovsky, 1998).Although these studies implicate high intensity locomotor activity (i.e., exercise) as the behavioral stimulus critical for phase resetting in response to at least some arousing stimuli, the contribution that sleep loss or nonspecific arousal makes to the phase shifting process, independent of locomotion, has not been adequately resolved. Animals that run little after an arousing stimulus may fail to shift because they do not stay awake, whereas the occasional...

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Section: Clock Resetting By Sleep Deprivation Is Rapidsupporting

confidence: 68%

Circadian Clock Resetting by Sleep Deprivation without Exercise in the Syrian Hamster

2000

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“…In Arvicanthis niloticus, one peak in peptide expression at ExT 9 is seen in LD 12:12, after which levels steadily decrease until 24 h later, evenly distributed over the whole SCN (Katona et al, 1998). As Schwartz et al (1994) already noted, c-Fos immunoreactivity patterns in the vlSCN seem to be species-specific, and might be coupled to the intrinsic properties of the circadian system. In order to address the comparative differences seen in c-Fos expression, we studied c-Fos expression in the Common vole.…”

Section: C-fos Under Ld Conditionsmentioning

confidence: 68%

“…Alternatively, a peak in C-FOS expression in the dmSCN is reported at 7 h into the subjective day (Chambille et al, 1993). Several reports state that no circadian rhythm in vlSCN c-Fos peptide or mRNA expression is seen under constant dark conditions (Schwartz et al, 1994;Sumová et al, 1998Sumová et al, , 2000Sumová et al, , 2005. When housed in continuous light, C-FOS immunoreactivity in the vlSCN is high around midnight (Earnest et al, 1992) or several hours later (Sumová et al, 2005).…”

Section: C-fos Under Constant Lighting Conditionsmentioning

confidence: 99%

“…Peaks in vlSCN C-FOS peptide expression are reported at External Time (ExT) 8-11 (Kononen et al, 1990;Colwell & Foster, 1992;Schwartz et al, 1994) under LD 12:12 or around dawn in short and long photoperiods (Jác et al, 2000). In general terms, c-Fos in the vlSCN is high during the light period and low in the dark (Earnest et al, 1990, Aronin et al, 1990, Kononen et al, 1990, Colwell & Foster, 1992, Schwartz et al, 1994. Some studies on c-Fos expression under LD conditions report on dmSCN c-Fos expression, while others (Jác et al, 2000) report almost identical expression profiles in the vlSCN and dmSCN peptide levels.…”

Section: C-fos Under Ld Conditionsmentioning

confidence: 99%

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Circadian Rhythms of C‐FOS Expression in the Suprachiasmatic Nuclei of the Common Vole (Microtus arvalis)

Veen

Pol-Meijer

Jansen

et al. 2008

Chronobiology International

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The suprachiasmatic nuclei of the hypothalamus (SCN) are the master circadian clock in mammals. Transcriptional activity in this master clock has a marker in the immediate-early gene c-Fos. Within the SCN, distinct differences in c-Fos in the ventrolateral and the dorsomedial SCN have been reported for rodent species such as rats, mice and hamsters.We studied C-FOS expression in the Common vole (Microtus arvalis) SCN under LD 12:12h and under constant dim light conditions. In the vole dorsomedial SCN rhythmic C-FOS expression was seen in LD with a clear peak in the middle of the light period. Under constant dim light conditions we report constitutive, non-rhythmic expression of C-FOS in the dorsomedial SCN. This pattern is consistent with circadian organization of behavioral activity, which is weak in voles, and may be lost under constant dim light conditions. In the ventrolateral SCN, under LD conditions we observed a rise in C-FOS expression prior to lights-on followed by peak expression at lights-on. Another peak was seen at lights-off. In an additional experiment we subjected animals to LD 16:8 to test the hypothesis that the dawn and dusk peaks in ventrolateral C-FOS expression change phase along with the photoperiod.

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“…According to recent investigations, FLI-labeled neurons have been observed following sexual activity [24], immobilization stress [27], conditioned and unconditioned aversive stimuli [22], baroreceptor activation [16], temporal regulation of light [26], somatic stimulation [28], et al It is generally accepted that various stimulations cause FLI-labeled neuron expression at the spinal cord level, and c-fos mRNA appears within 30 min of stimulation [8].…”

Section: Introductionmentioning

confidence: 99%

Temporal Analysis of Increases in c-Fos Expression in Rat Spinal Trigeminal Nucleus Complex by Selective Stimulation of C-afferent Fibers.

Tomioka

1995

Acta Histochem. Cytochem

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This study evaluates temporal increases in the expression of Fos-like immunoreactive labeled neurons (FLI-labeled neurons) in the spinal trigeminal nucleus complex (STNC) following selective stimulation of C-fibers. Selective stimulating C-fibers were obtained using low doses (10 mg/kg b.w.) of capsaicin injection.Under sodium pentobarbital anaesthesia, 1% capsaicin was administered into the rats left infraorbital region. At periods of 30 min, 1, 3, 6, 9 and 12 hr after capsaicin injection, rats were anesthetized and killed for immunohistochemical examination of Fos-like protein products.The results showed that FLI-labeled neurons in the STNC appeared within 30 min after induction of capsaicin injection into the rat trigeminal region, reached peak increases at 3 hr, and disappeared between 9 and 12 hr.This time course was similar to dental treatment results which probably stimulated both A-delta and C fibers. According to previous studies, both A-delta and C fibers are essential to conduct impulses of nociceptive stimulation. But, this theory does not apply to the time course of FLI-labeled neuron expression in the STNC. In conclusion, this study showed that the time course of FLI-labeled neuron expression in the STNC was unchanged in stimulated or non-stimulated A-delta fibers.

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Temporal regulation of light-induced fos and fos-like protein expression in the ventrolateral subdivision of the rat suprachiasmatic nucleus

Cited by 93 publications

References 35 publications

Circadian Clock Resetting by Sleep Deprivation without Exercise in the Syrian Hamster

Circadian Clock Resetting by Sleep Deprivation without Exercise in the Syrian Hamster

Circadian Rhythms of C‐FOS Expression in the Suprachiasmatic Nuclei of the Common Vole (Microtus arvalis)

Temporal Analysis of Increases in c-Fos Expression in Rat Spinal Trigeminal Nucleus Complex by Selective Stimulation of C-afferent Fibers.

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