Temporal Regulation in the Individual Gonyaulax Cell

Hastings, J. W.; Krasnow, R.

doi:10.1007/978-3-642-67916-2_91

Cited by 4 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sweeney (1960) measured the rhythm of photosynthetic capacity in single cells of Gonyaulax polyedra for 14 hr and found that the lack of apparent rhythmicity in the cell population is a true reflection of behavior of each cell, not the result of desynchronization. The results of Hastings and Krasnow (1981) suggest the same explanation for the bioluminescence rhythm of Gonyaulax. In the eclosion rhythm of Drosophila pseudoobscura, rhythmicity in a population is believed to be caused by synchronization of the rhythm in each individual (Pittendrigh and Bruce, 1957).…”

Section: Discussionsupporting

confidence: 54%

See 1 more Smart Citation

Circadian Rhythmicity within Single Cells of Paramecium bursaria

1987

View full text Add to dashboard Cite

Cell populations of Paramecium bursaria show mating reactivity in the light period, but not in the dark period, when exposed to a light-dark cycle (LD 12:12). After they are transferred to constant-light (LL) conditions (1,000 lux), they continue to show a circadian rhythm of mating reactivity. The rhythm gradually dampens in LL so that mating reactivity in populations becomes arrhythmic in LL within 2 weeks. We wanted to know whether the arrhythmicity of this population was due to the absence of circadian rhythmicity within each individual cell, or merely due to asynchrony of a population of individually rhythmic cells. Therefore, single cells were isolated randomly from an arrhythmic population that had been in LL for a long time. Then the mating reactivity of these single cells was individually tested every 3 hr for 2 days. Each single cell showed a circadian mating rhythm in LL. This shows that the disappearance of the mating rhythm in cell populations under LL is not caused by disappearance of circadian rhythmicity within individual cells, but is due to desynchronization among cells in a population. When an arrhythmic population in LL is darkened for 9 hr, the mating reactivity rhythm of the cell population reappears. This occurs by resynchronization of the rhythms among individual cells, as can be shown by exposing single cells to pulses of 9 hr of darkness. This dark treatment causes phase shifts of single-cell rhythms, and a phase response curve is obtained for this stimulus. This phase-shifting behavior explains the efficacy of 9-hr dark pulses in restoring the population's rhythm.

show abstract

Section: Discussionsupporting

confidence: 54%

“…In addition, circadian rhythms have been measured from single isolated cells of Acetabularia (Schweiger et al, 1964) and Gonyaulax (Sweeney, 1960 ;Hastings and Krasnow, 1981), but in the case of Gonyaulax, only one cycle has been measured from single cells.…”

mentioning

confidence: 99%

Circadian Rhythmicity within Single Cells of Paramecium bursaria

1987

View full text Add to dashboard Cite

show abstract

“…Spontaneous flashing appears to be a stochastic process that is not due to cell collisions (Hastings and Krasnow , Krasnow et al. , Latz and Lee ) and occurs at a maximum rate of 0.002–0.03 flashes · min −1 · cell −1 , depending on species (Krasnow et al.…”

mentioning

confidence: 99%

Pharmacological investigation of the bioluminescence signaling pathway of the dinoflagellate Lingulodinium polyedrum: evidence for the role of stretch‐activated ion channels

Jin

Klima

Deane

et al. 2013

Journal of Phycology

View full text Add to dashboard Cite

Dinoflagellate bioluminescence serves as a whole-cell reporter of mechanical stress, which activates a signaling pathway that appears to involve the opening of voltage-sensitive ion channels and release of calcium from intracellular stores. However, little else is known about the initial signaling events that facilitate the transduction of mechanical stimuli. In the present study using the red tide dinoflagellate Lingulodinium polyedrum (Stein) Dodge, two forms of dinoflagellate bioluminescence, mechanically stimulated and spontaneous flashes, were used as reporter systems to pharmacological treatments that targeted various predicted signaling events at the plasma membrane level of the signaling pathway. Pretreatment with 200 μM Gadolinium III (Gd(3+) ), a nonspecific blocker of stretch-activated and some voltage-gated ion channels, resulted in strong inhibition of both forms of bioluminescence. Pretreatment with 50 μM nifedipine, an inhibitor of L-type voltage-gated Ca(2+) channels that inhibits mechanically stimulated bioluminescence, did not inhibit spontaneous bioluminescence. Treatment with 1 mM benzyl alcohol, a membrane fluidizer, was very effective in stimulating bioluminescence. Benzyl alcohol-stimulated bioluminescence was inhibited by Gd(3+) but not by nifedipine, suggesting that its role is through stretch activation via a change in plasma membrane fluidity. These results are consistent with the presence of stretch-activated and voltage-gated ion channels in the bioluminescence mechanotransduction signaling pathway, with spontaneous flashing associated with a stretch-activated component at the plasma membrane.

show abstract

“…The light emission involves spontaneous action-potential-triggered flashes (Eckert, 1965), which are most frequent during the mid-dark phase, and a steady glow reaching a maximum shortly before subjective dawn. The daily endogenous control of different physiological activities is a common feature of all organisms with one or more circadian clocks and can be studied in Gonyaulax polyedra at the level of a single cell (McMurry and Hastings, 1972;Hastings and Krasnow, 1981). Populations of these algae show also more complex circadian aspects commonly associated with higher organisms, such as distinct stages of activity and rest, different utilization of spatial environment during night and day, and other behavioral strategies such as swarming and self-selection of environmental conditions (e.g., light intensity).…”

mentioning

confidence: 99%

A Circadian Rhythm of Population Behavior in Gonyaulax polyedra

1989

View full text Add to dashboard Cite

Like other flagellates, Gonyaulax polyedra exhibits diurnal vertical migration and pattern formation. Shape and size of the aggregations depend on container type, light intensity, and cell density. In Petri dishes, cells form oval "swarms"; within these, cells move downward in the highly dense center and rise up at the periphery. We have investigated the daily rhythm of this swarming activity in Petri dishes illuminated from the side, using timelapse video recordings. At night, a "lawn" of cells forms at the bottom of the dish toward the light source (independent of light intensity). Before dawn, cells rise toward the surface and aggregate in swarms. The daily vertical migration occurs independent of light direction and intensity. The diurnal swarms, however, form every day at the same location within the dish, at a distance from the light that depends on light intensity, indicating a self-selection of light intensity. In constant light and temperature and with negligible vertical nutrient differences, all aspects of the rhythm continue to oscillate for up to 3 weeks, when the rhythm of the population becomes desynchronized. Under cycles of bright white-dim red light (WR), cell entrain to WR 10: 10 but free run in WR 8:8 and shorter cycles, showing relative coordination (von Holst, 1939) to the driving light cycle. They also entrain to the 24-hr multiple of WR 6:6. Under nonentrained conditions, swarming activity is still influenced by light changes, and in spite of the apparent free run, the phasing of the averaged activity varies systematically with different T-cycle frequencies.

show abstract

Temporal Regulation in the Individual Gonyaulax Cell

Cited by 4 publications

References 9 publications

Circadian Rhythmicity within Single Cells of Paramecium bursaria

Circadian Rhythmicity within Single Cells of Paramecium bursaria

Pharmacological investigation of the bioluminescence signaling pathway of the dinoflagellate Lingulodinium polyedrum: evidence for the role of stretch‐activated ion channels

A Circadian Rhythm of Population Behavior in Gonyaulax polyedra

Contact Info

Product

Resources

About