Temporal profiling of the heat‐stable proteome during late maturation of <i>Medicago truncatula</i> seeds identifies a restricted subset of late embryogenesis abundant proteins associated with longevity

Châtelain, Emilie; Hundertmark, Michaela; Leprince, Olivier; Gall, Sophie Le; Satour, Pascale; Deligny-Penninck, Stéphanie; Rogniaux, Hélène; Buitink, Julia

doi:10.1111/j.1365-3040.2012.02501.x

Cited by 126 publications

(177 citation statements)

References 48 publications

(85 reference statements)

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“…Water contents are expressed on a dry weight basis. DT and longevity were determined according to Chatelain et al (2012) on three biological replicates of 50 seeds that were rapidly dried at 43% RH under airflow and harvested from a pool of three plants each. Longevity was determined for each of the 49 developmental stages of each environmental growth condition as the storage time that was needed to decrease the germination of the seed population to 50% (P50), derived from survival curves of percentage of final germination over storage time (75% RH, 35°C).…”

Section: Plant Materials and Physiological Analysesmentioning

confidence: 99%

“…These traits rely on the remarkable capacity of seeds to undergo complete desiccation without loss of viability (desiccation tolerance [DT]) and to remain alive for extended periods of time when stored in the dry state (longevity) . While DT is acquired during seed filling, longevity progressively increases during the final phase of seed maturation (Probert et al, 2009;Chatelain et al, 2012). Seed longevity is the ultimate survival mechanism for the plant in order to bridge unfavorable conditions.…”

Section: Introductionmentioning

confidence: 99%

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Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Righetti¹,

Vu²,

Pelletier³

et al. 2015

Plant Cell

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111

191

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Seed longevity, the maintenance of viability during storage, is a crucial factor for preservation of genetic resources and ensuring proper seedling establishment and high crop yield. We used a systems biology approach to identify key genes regulating the acquisition of longevity during seed maturation of Medicago truncatula. Using 104 transcriptomes from seed developmental time courses obtained in five growth environments, we generated a robust, stable coexpression network (MatNet), thereby capturing the conserved backbone of maturation. Using a trait-based gene significance measure, a coexpression module related to the acquisition of longevity was inferred from MatNet. Comparative analysis of the maturation processes in M. truncatula and Arabidopsis thaliana seeds and mining Arabidopsis interaction databases revealed conserved connectivity for 87% of longevity module nodes between both species. Arabidopsis mutant screening for longevity and maturation phenotypes demonstrated high predictive power of the longevity cross-species network. Overrepresentation analysis of the network nodes indicated biological functions related to defense, light, and auxin. Characterization of defense-related wrky3 and nf-x1-like1 (nfxl1) transcription factor mutants demonstrated that these genes regulate some of the network nodes and exhibit impaired acquisition of longevity during maturation. These data suggest that seed longevity evolved by co-opting existing genetic pathways regulating the activation of defense against pathogens.

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Section: Plant Materials and Physiological Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Righetti¹,

Vu²,

Pelletier³

et al. 2015

Plant Cell

Self Cite

111

191

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show abstract

“…During the maturation phase, mechanisms are put in place to prevent and repair desiccation damage, as well as to allow survival of seeds in the dry state (3)(4)(5). Nonetheless, drying of seeds on mother plants can occur prematurely in drought conditions, leading to low-quality seeds (6)(7)(8).…”

mentioning

confidence: 99%

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Châtelain

Satour

Laugier

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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101

103

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Seeds are in a natural oxidative context leading to protein oxidation. Although inevitable for proper progression from maturation to germination, protein oxidation at high levels is detrimental and associated with seed aging. Oxidation of methionine to methionine sulfoxide is a common form of damage observed during aging in all organisms. This damage is reversible through the action of methionine sulfoxide reductases (MSRs), which play key roles in lifespan control in yeast and animal cells. To investigate the relationship between MSR capacity and longevity in plant seeds, we first used two Medicago truncatula genotypes with contrasting seed quality. After characterizing the MSR family in this species, we analyzed gene expression and enzymatic activity in immature and mature seeds exhibiting distinct quality levels. We found a very strong correlation between the initial MSR capacities in different lots of mature seeds of the two genotypes and the time to a drop in viability to 50% after controlled deterioration. We then analyzed seed longevity in Arabidopsis thaliana lines, in which MSR gene expression has been genetically altered, and observed a positive correlation between MSR capacity and longevity in these seeds as well. Based on our data, we propose that the MSR repair system plays a decisive role in the establishment and preservation of longevity in plant seeds.seed deterioration | seed viability | redox systems | antioxidant enzymes S uccessful crop implantation is crucial for maximal plant yield and relies on seed physiological quality, which is acquired at the end of development, during maturation. Quality is expressed by the capacity of seeds to germinate in a fast and homogenous manner before and after storage (seed longevity) and to provide proper seedling establishment in various environments (seed vigor). Quality is controlled by genetic traits and varies greatly among species and cultivars. The establishment of quality also depends on the prevailing environmental conditions during seed development and storage (1, 2).During the maturation phase, mechanisms are put in place to prevent and repair desiccation damage, as well as to allow survival of seeds in the dry state (3-5). Nonetheless, drying of seeds on mother plants can occur prematurely in drought conditions, leading to low-quality seeds (6-8). Thus, in the context of a warming climate, producing good-quality seeds represents a global challenge to sustain current yield levels (9, 10).The mechanisms underlying seed quality are controlled in part by abscisic acid (ABA), the phytohormone involved in plant responses to dehydration that is produced during seed maturation. Of note, ABA triggers the accumulation of late-embryogenesis abundant proteins, heat-shock proteins (HSPs), and storage proteins (11). Late-embryogenesis abundant proteins and HSPs are thought to primarily protect cell structures when water is removed. Reserve proteins, through their sensitivity to reactive oxygen species (ROS), play a determinant role in limiting oxidative damage...

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“…Mechanisms for longevity in the dry state provide stabilization of the biological entity for long periods of time by slowing down deteriorative reactions (Buitink and Leprince, 2004;Chatelain et al, 2012). As discussed above, Chl degradation and changes in the proportion of arabinose in the cell wall are highly correlated with longevity in resurrection plants and seeds.…”

Section: Mechanisms For Longevity In the Dry Statementioning

confidence: 93%

“…LEA protein levels in maturing Arabidopsis and M. truncatula seeds are positively correlated with an increase in seed longevity (Hundertmark et al, 2011;Chatelain et al, 2012). In Arabidopsis seeds and X. viscosa leaves, LEA proteins are broadly distributed in subcellular compartments, reflecting their protective role in the various cellular membranes (Candat et al, 2014;Costa et al, 2017).…”

Section: Mechanisms For Longevity In the Dry Statementioning

confidence: 99%

Orthodox Seeds and Resurrection Plants: Two of a Kind?

Costa

Cooper

Hilhorst³

et al. 2017

Plant Physiol.

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Temporal profiling of the heat‐stable proteome during late maturation of Medicago truncatula seeds identifies a restricted subset of late embryogenesis abundant proteins associated with longevity

Abstract: ABSTRACT

Cited by 126 publications

References 48 publications

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Orthodox Seeds and Resurrection Plants: Two of a Kind?

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