Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve

Assia, Ehud I.; Rosner, Mordechai; Belkin, Michael; Solomon, Albert; Schwartz, Michal

doi:10.1016/0006-8993(89)91240-7

Cited by 95 publications

(50 citation statements)

References 14 publications

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“…Photobiomodulation by light in the red to near-IR range (630-1,000 nm) using low-energy lasers or light-emitting diode (LED) arrays has been shown to accelerate wound healing, improve recovery from ischemic injury in the heart, and attenuate degeneration in the injured optic nerve (19)(20)(21)(22)(23)(24). At the cellular level, photoirradiation at low fluences can generate significant biological effects, including cellular proliferation, collagen synthesis, and the release of growth factors from cells (22,25,26).…”

mentioning

confidence: 99%

Therapeutic photobiomodulation for methanol-induced retinal toxicity

Eells

Henry

Summerfelt

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

341

262

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Methanol intoxication produces toxic injury to the retina and optic nerve, resulting in blindness. The toxic metabolite in methanol intoxication is formic acid, a mitochondrial toxin known to inhibit the essential mitochondrial enzyme, cytochrome oxidase. Photobiomodulation by red to near-IR radiation has been demonstrated to enhance mitochondrial activity and promote cell survival in vitro by stimulation of cytochrome oxidase activity. The present studies were undertaken to test the hypothesis that exposure to monochromatic red radiation from light-emitting diode (LED) arrays would protect the retina against the toxic actions of methanolderived formic acid in a rodent model of methanol toxicity. Using the electroretinogram as a sensitive indicator of retinal function, we demonstrated that three brief (2 min, 24 s) 670-nm LED treatments (4 J͞cm 2 ), delivered at 5, 25, and 50 h of methanol intoxication, attenuated the retinotoxic effects of methanolderived formate. Our studies document a significant recovery of rod-and cone-mediated function in LED-treated, methanol-intoxicated rats. We further show that LED treatment protected the retina from the histopathologic changes induced by methanolderived formate. These findings provide a link between the actions of monochromatic red to near-IR light on mitochondrial oxidative metabolism in vitro and retinoprotection in vivo. They also suggest that photobiomodulation may enhance recovery from retinal injury and other ocular diseases in which mitochondrial dysfunction is postulated to play a role. D ecrements in mitochondrial function have been postulated to be involved in the pathogenesis of numerous retinal and optic nerve diseases, including age-related macular degeneration, diabetic retinopathy, and Leber's hereditary optic neuropathy (1-3). Decrements in mitochondrial function have also been postulated to be involved in the pathogenesis in methanol intoxication (4-7). Methanol intoxication produces toxic injury to the retina and optic nerve, frequently resulting in blindness. A toxic exposure to methanol typically results in the development of formic acidemia, metabolic acidosis, visual toxicity, coma, and, in extreme cases, death (8, 9). Visual disturbances generally develop between 18 and 48 h after methanol ingestion and range from misty or blurred vision to complete blindness. Both acute and chronic methanol exposure have been shown to produce retinal dysfunction and optic nerve damage clinically (8-10) and in experimental animal models (11)(12)(13)(14).Formic acid is the toxic metabolite responsible for the retinal and optic nerve toxicity produced in methanol intoxication (4-7, 15). Formic acid is a mitochondrial toxin that inhibits cytochrome c oxidase, the terminal enzyme of the mitochondrial electron transport chain of all eukaryotes (16,17). Cytochrome oxidase is an important energy-generating enzyme critical for the proper functioning of almost all cells, especially those of highly oxidative organs, including the retina and brain (18). Previous studies in...

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mentioning

confidence: 99%

Therapeutic photobiomodulation for methanol-induced retinal toxicity

Eells

Henry

Summerfelt

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

341

262

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“…In those early studies we demonstrated that the low energy He-Ne laser treatment, if commences immediately after injury, delays the posttraumatic degeneration but does not prevent it (Assia et al, 1989) nor does it initiate axonal growth .…”

Section: Forewordmentioning

confidence: 90%

“…In conducting research using animals, the investigator(s) adhered to the "Guide for the Care and A few years ago, we have initiated the use of low power helium-neon (He-Ne) laser (632.8 nm) for the treatment of injured nerves of the central nervous system (CNS) and of the peripheral nervous system (PNS) ; Schwartz et al, 1987; Assia et al, 1989). In those early studies we demonstrated that the low energy He-Ne laser treatment, if commences immediately after injury, delays the posttraumatic degeneration but does not prevent it (Assia et al, 1989) nor does it initiate axonal growth .…”

Section: Forewordmentioning

confidence: 99%

Low Power Laser Irradiation Effect with Emphasis on Injured Neural Tissues.

Schwartz¹

1993

Self Cite

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“…Photobiomodulation effects of Low-level light irradiation (LLLI) on regeneration have been reported in skin [1] [2], nerve [3], skeletal muscle tissues [4] and bone [5]. LLLI can be used as a efficiently tool for the preconditioning of bone marrow mesenchymal stem cells (MSCs), which derived from bone marrow and received wildly attentions in regeneration medicine.…”

Section: Introductionmentioning

confidence: 99%

The Effect of 635 nm Red Laser Irradiation on Proliferation of Bone Marrow Stem Cells

Peng¹

2016

OPJ

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Photobiomodulation effects of Low-level light irradiation (LLLI) on regeneration have been reported in skin, nerve, and skeletal muscle tissues and bone. Bone Mesenchymal stem cells (BMSCs)are derived from bone marrow, which exhibited a fibroblast-like appearance, and could differentiate in vitro into different lineages. However, there is a reciprocal relationship between growth and osteogenic differentiation in MSCs. Therefore, it's important to investigate the effect of LLLI on BMSCs. The aim of our study was to investigate the proliferation effect of 635 nm red laser light on bone marrow MSCs with or without osteogenic supplements. Bone marrow was collected from the 4-week-old Sprague-Dawley rats femur and tibiae. MSCs with and without osteogenic supplements both were divided into three groups. A continuous 635 nm wavelength red light diode laser (a power output of 960 mW) was used in the study. The size of light spot was 35mm in diameter. Irradiation was performed every other day since the half of medium was changed to osteogenic differentiation media (ODM). The first irradiation day was set as 0 day. The duration of each irradiation for red light was calculated at 10 seconds for 1 J/cm 2 , 20 seconds for 2 J/cm 2 . Two of these groups were used as controls: MSCs incubated in DMEM without irradiation (control 1), MSCs incubated in ODM without irradiation (control 2). Cellular proliferation was evaluated by using WST-8. Cell viability was assessed with WST-8 kit at 2, 4, 6 and 8 days, respectively. At 4, 6 and 8 days, groups cultured with DMEM showed significantly higher viabilities than that in groups with ODM. In groups with DMEM, red light at all doses significantly stimulated cell viability as compared with the control 1. Groups irradiated at 1 and 2 J/cm 2 had more effective proliferation on 4 (P < 0.01) and 6 days (P < 0.05), when compared with the control 1. In groups with ODM, control 2 and the irradiated groups showed similar proliferation speeds. In conclusion, we can find that red light can promote proliferation of MSCs cultured in normal media, and suppress proliferation of MSCs cultured in ODM.

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Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve

Cited by 95 publications

References 14 publications

Therapeutic photobiomodulation for methanol-induced retinal toxicity

Therapeutic photobiomodulation for methanol-induced retinal toxicity

Low Power Laser Irradiation Effect with Emphasis on Injured Neural Tissues.

The Effect of 635 nm Red Laser Irradiation on Proliferation of Bone Marrow Stem Cells

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