Temporal gene expression during differentiation of human embryonic stem cells and embryoid bodies

Dvash, Tamar; Mayshar, Yoav; Darr, Henia; McElhaney, Michael; Barker, Douglas S.; Yanuka, Ofra; Kotkow, Karen; Rubin, Lee L.; Benvenisty, Nissim; Eiges, Rachel

doi:10.1093/humrep/deh529

Cited by 124 publications

(100 citation statements)

References 26 publications

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“…differentiate into a large number of somatic cell types (Amit et al, 2000;Xu et al, 2001). The upregulated FGF signaling components identified in ESCs include FGF-2, FGF-11, and FGF-13, as well as all four FGF receptors (FGFR; Dvash et al, 2004;Skottman et al, 2005). The expression of the FGFRs in undifferentiated cells follows a specific pattern, with FGFR1 being the most abundant receptor and the other receptors showing a lower expression in the following order: FGFR3 > FGFR4 > FGFR2 (Dvorak et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells

Morito

Kida

Suzuki

et al. 2009

Archives of Histology and Cytology

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in the presence of bFGF into immunocompromised mice revealed the formation of bone, cartilage, and adipose tissue. The donor hDPC-derived cells were labeled in the bone tissues located near the PLGA in the subcutaneous tissues of recipient mice using a humanspecific Alu probe. When cultured with a serum-free medium containing bFGF, the hDPCs strongly expressed STRO-1 immunoreactive products and sustained selfrenewal, and thus were almost identical in differentiation potential and proliferation activity to hDPCs cultured with the medium containing serum and bFGF. The present results suggest that the hDPCs cultured in the presence of bFGF irrespective of the presence or absence of the bovine serum are rich in mesenchymal stem cells or progenitor cells and useful for cell-based therapies to treat dental diseases.

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Section: Discussionmentioning

confidence: 99%

Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells

Morito

Kida

Suzuki

et al. 2009

Archives of Histology and Cytology

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“…For EB differentiation, hESCs were harvested via 0.05% trypsin (Invitrogen) supplemented with 0.5 mM CaCl 2 , and the resultant cell aggregates were cultured in nonadherent dishes (BD Biosciences) for up to 30 d in maintenance medium lacking FGF2 (medium changes performed as necessary) (Itskovitz-Eldor et al 2000;Dvash et al 2004). At appropriate time points, RNA was extracted into TRIzol, and aliquots of total RNA from day 0 (hESC) and day 15 (EB) were subjected to miRNA library construction as follows.…”

Section: Methodsmentioning

confidence: 99%

Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells

Morin¹,

O’Connor²,

Griffith³

et al. 2008

Genome Res.

997

919

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MicroRNAs (miRNAs) are emerging as important, albeit poorly characterized, regulators of biological processes. Key to further elucidation of their roles is the generation of more complete lists of their numbers and expression changes in different cell states. Here, we report a new method for surveying the expression of small RNAs, including microRNAs, using Illumina sequencing technology. We also present a set of methods for annotating sequences deriving from known miRNAs, identifying variability in mature miRNA sequences, and identifying sequences belonging to previously unidentified miRNA genes. Application of this approach to RNA from human embryonic stem cells obtained before and after their differentiation into embryoid bodies revealed the sequences and expression levels of 334 known plus 104 novel miRNA genes. One hundred seventy-one known and 23 novel microRNA sequences exhibited significant expression differences between these two developmental states. Owing to the increased number of sequence reads, these libraries represent the deepest miRNA sampling to date, spanning nearly six orders of magnitude of expression. The predicted targets of those miRNAs enriched in either sample shared common features. Included among the high-ranked predicted gene targets are those implicated in differentiation, cell cycle control, programmed cell death, and transcriptional regulation.

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“…For voltage-gated ion channels, a total of 36, 19, and 49 genes on the U133A chips were identified as Ca v , Na v , or K v channel genes, respectively. For inspection, their expression profile is extracted in Figure 5A and further summarized in Figures 5B and 5C by normalizing signals to the average expression level of the entire microarray in a manner similar to that of cytokines and their receptors in hESCs, as recently reported by Dvash et al [24]. Our data indicate that among the total 104 voltage-gated ion channel genes mentioned above, only the transcripts of 3, 1, and 5 Ca v , Na v , and K v genes were significantly expressed, as defined by Affymetrix.…”

Section: Microarray Analysis Of Ion Channel Genes In Hescsmentioning

confidence: 99%

Electrophysiological Properties of Pluripotent Human and Mouse Embryonic Stem Cells

et al. 2005

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Pluripotent embryonic stem cells (ESCs) possess promising potential for cell-based therapies, but their electrophysiological properties have not been characterized. Here we describe the presence of ionic currents in mouse (m) and human (h) ESCs and their physiological function. In mESCs, tetraethylammonium (TEA) -sensitive depolarization-activated delayed rectifier K + currents (IK DR ) (8.6 ± 0.9 pA/pF at +40 mV; IC 50 = 1.2 ± 0.3 mM), which contained components sensitive to 4-aminopyridine (4-AP) (IC 50 = 0.5 ± 0.1 mM) and 100 nM Ca 2+ -activated K + current (IK Ca ) blocker iberiotoxin (IBTX), were detected in 52.3% of undifferentiated cells. IK DR was similarly present in hESCs (~100%) but with an approximately sixfold higher current density (47.5 ± 7.9 pA/pF at +40 mV). When assayed by bromodeoxyurindine incorporation, application of TEA, 4-AP, or IBTX significantly reduced the proliferation of mESCs and hESCs in a dose-dependent manner (p < .05). A hyperpolarization-activated inward current (I h ) (−2.2 ± 0.4 pA/pF at −120 mV) was detected in 23% of mESCs but not hESCs. Neither Na v nor Ca v currents were detected in mESCs and hESCs. Microarray and reverse transcription-polymerase chain reaction analyses identified several candidate genes for the ionic currents discovered. Collectively, our results indicate that pluripotent ESCs functionally express several specialized ion channels and further highlight similarities and differences between the two species. Practical considerations for the therapeutic use of ESCs are discussed. Stem Cells 2005;23:1526-1534

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Temporal gene expression during differentiation of human embryonic stem cells and embryoid bodies

Abstract: The present study provides a molecular basis for the identity of human ES cells and demonstrates that during their in vitro differentiation they express embryonic specific genes in a stage specific manner.

Cited by 124 publications

References 26 publications

Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells

Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells

Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells

Electrophysiological Properties of Pluripotent Human and Mouse Embryonic Stem Cells

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