Temporal expression of retinoic acid receptors in hamster fetus during organogenesis and alteration by retinoic acid treatment

Colón-Teicher, Luz; Dugyala, Raviprakash R.; Sharma, Raghubir P.

doi:10.1016/0742-8413(96)00022-9

Cited by 3 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results show that the RARG gene expression ratio was highest between 37 and 52 dph, which is concomitant with the onset of the typical adult skin development in gilthead sea bream; and in concordance with that reported in European sea bass between 10-42 dph [ 13 ]. The modulation of RARG expression by hypervitaminosis A has been demonstrated in mammals [ 44 ] and fishes [ 16 , 17 , 32 ]. Nevertheless, no regulation of RARG expression by hypervitaminosis A was detected in gilthead sea bream larvae at 18 dph.…”

Section: Discussionmentioning

confidence: 99%

Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A

et al. 2011

View full text Add to dashboard Cite

BackgroundVitamin A (VA) has a key role in vertebrate morphogenesis, determining body patterning and growth through the control of cell proliferation and differentiation processes. VA regulates primary molecular pathways of those processes by the binding of its active metabolite (retinoic acid) to two types of specific nuclear receptors: retinoic acid receptors (RARs) and retinoid X receptors (RXRs), which promote transcription of downstream target genes. This process is well known in most of higher vertebrates; however, scarce information is available regarding fishes. Therefore, in order to gain further knowledge of fish larval development and its disruption by nutritional VA imbalance, the relative expression of some RARs and RXRs, as well as several genes involved in morpho- and skeletogenesis such as peroxisome proliferator-activated receptors (PPARA, PPARB and PPARG); retinol-binding protein (RBP); insulin-like growth factors I and II (IGF1 and IGF2, respectively); bone morphogenetic protein 2 (Bmp2); transforming growth factor β-1 (TGFB1); and genes encoding different extracellular matrix (ECM) proteins such as matrix Gla protein (mgp), osteocalcin (bglap), osteopontin (SPP1), secreted protein acidic and rich in cysteine (SPARC) and type I collagen α1 chain (COL1A1) have been studied in gilthead sea bream.ResultsDuring gilthead sea bream larval development, specific expression profiles for each gene were tightly regulated during fish morphogenesis and correlated with specific morphogenetic events and tissue development. Dietary hypervitaminosis A during early larval development disrupted the normal gene expression profile for genes involved in RA signalling (RARA), VA homeostasis (RBP) and several genes encoding ECM proteins that are linked to skeletogenesis, such as bglap and mgp.ConclusionsPresent data reflects the specific gene expression patterns of several genes involved in larval fish RA signalling and skeletogenesis; and how specific gene disruption induced by a nutritional VA imbalance underlie the skeletal deformities. Our results are of basic interest for fish VA signalling and point out some of the potential molecular players involved in fish skeletogenesis. Increased incidences of skeletal deformities in gilthead sea bream fed with hypervitaminosis A were the likely ultimate consequence of specific gene expression disruption at critical development stages.

show abstract

Section: Discussionmentioning

confidence: 99%

Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Both vitamin A deficiency and excess have profound adverse consequences on morphogenesis and organogenesis in the vertebrate embryo [ 9 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ], implying that normally there is a tight regulation of the endogenous vitamin A-active form, all- trans -RA. Exogenously applied vitamin A-active compounds have been used to manipulate and perturb normal embryonic development, and have provided evidence that almost every organ or tissue system can be severely affected by RA if the embryo is treated with it at a critical time in development [ 39 , 40 , 41 , 42 , 43 , 44 ]. These studies have revealed that RA via its nuclear receptors can also regulate the expression of developmental genes.…”

Section: Vitamin a In Embryonic Developmentmentioning

confidence: 99%

Vitamin A–Not for Your Eyes Only: Requirement for Heart Formation Begins Early in Embryogenesis

Zile

2010

Nutrients

View full text Add to dashboard Cite

Vitamin A insufficiency has profound adverse effects on embryonic development. Major advances in understanding the role of vitamin A in vertebrate heart formation have been made since the discovery that the vitamin A active form, all-trans-retinoic acid, regulates many genes, including developmental genes. Among the experimental models used, the vitamin A-deficient avian embryo has been an important tool to study the function of vitamin A during early heart formation. A cluster of retinoic acid-regulated developmental genes have been identified that participate in building the heart. In the absence of retinoic acid the embryonic heart develops abnormally leading to embryolethality.

show abstract

“…Both vitamin A deficiency and excess have profound effects on morphogenesis and organogenesis of the vertebrate embryo (reviewed in 4, 5). Studies with exogenously applied retinoids have been used to manipulate and perturb normal embryonic development and have prOVided evidence that almost every organ or tissue system can be severely affected by retinoic acid if the embryo is treated with it at a crucial time in development (23)(24)(25)(26)(27)(28).…”

Section: Requirement Of Vitamin a For Normal Embryonic And Fetal Devementioning

confidence: 99%

Vitamin A Requirement for Early Cardiovascular Morphogenesis Specification in the Vertebrate Embryo: Insights from the Avian Embryo

Zile

2004

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Vitamin A is required throughout the life cycle, including crucial stages of embryonic and fetal development. With the identification of retinoic acid-specific nuclear transcription factors, the retinoid receptors, considerable advances have been made in understanding the molecular function of vitamin A. The requirement for vitamin A during early embryogenesis has successfully been examined in the vitamin A-deficient avian embryo during neurulation, when in the vertebrates crucial developmental decisions take place. These studies revealed that retinoic acid is essential during these early stages of embryogenesis for the initiation of organogenesis (i.e., formation of the heart). If retinoic acid is not present at this time, abnormal development ensues, leading to early embryonic death. Though the initial insult of the absence of vitamin A appears to be on the specification of cardiovascular tissues, subsequently all development is adversely affected and the embryo dies. Molecular and functional studies revealed that retinoic acid regulates the expression of the cardiogenic transcription factor GATA-4 and several heart asymmetry genes, which explains why the heart position is random in vitamin A-deficient quail embryos. During the crucial retinoic acid-requiring developmental window, retinoic acid transduces its signals to genes for heart morphogenesis via the receptors RARalpha2, RARgamma, and RXRalpha. Elucidation of the function of vitamin A during early embryonic development may lead to a better understanding of the cardiovascular birth defects prevalent in the Western world.

show abstract

Temporal expression of retinoic acid receptors in hamster fetus during organogenesis and alteration by retinoic acid treatment

Cited by 3 publications

References 30 publications

Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A

Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A

Vitamin A–Not for Your Eyes Only: Requirement for Heart Formation Begins Early in Embryogenesis

Vitamin A Requirement for Early Cardiovascular Morphogenesis Specification in the Vertebrate Embryo: Insights from the Avian Embryo

Contact Info

Product

Resources

About