Temporal Dynamics of Metabolic Acquisition in Grafted Engineered Human Liver Tissue

Abstract

Liver disease affects millions globally, and end‐stage liver failure is only cured by organ transplant. Unfortunately, there is a growing shortage of donor organs as well as inequitable access to transplants across populations. Engineered liver tissue grafts that supplement or replace native organ function can address this challenge. While engineered liver tissues have been successfully engrafted previously, the extent to which these tissues express human liver metabolic genes and proteins remains unknown. Here, it is built engineered human liver tissues and characterized their engraftment, expansion, and metabolic phenotype at sequential stages post‐implantation by RNA sequencing, histology, and host serology. Expression of metabolic genes is observed at weeks 1–2, followed by the cellular organization into hepatic cords by weeks 4–9.5. Furthermore, grafted engineered tissues exhibited progressive spatially restricted expression of critical functional proteins known to be zonated in the native human liver. This is the first report of engineered human liver tissue zonation after implantation in vivo, which can have important translational implications for this field.

Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ‐Carrageenan Hydroxyapatite Biocomposite as Angio‐Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair

“…Protein level expression of osteocalcin (OCN) and osteopontin (OPN) in MG-63 cells; VEGF and VEGF-R2 in EA.hy926 cells were quantitated using an ELISA procedure. [30,43] Total lysate from cells maintained in culture on biocomposites, Col-KA-HA and Col-KA-HA-LO was collected in RIPA buffer. Total protein content was quantified using bicinchonic acid assay.…”