“…Consequently, the Usuthu River is expected to support a higher biodiversity of aquatic invertebrates. Whether this is indeed the case, and how the biodiversity between the two systems compares to one another is unclear, as prior research in the PRF has focused largely on the Phongolo River (de Necker, Neswiswi, et al., 2020 ; Dube et al., 2015 ; Smit et al., 2016 ), its associated floodplain and the temporary wetlands of the area (de Necker et al., 2021 ; de Necker, Brendonck, et al., 2022 ; de Necker, Gerber, et al., 2022 ; de Necker, Manfrin, et al., 2020 ; Dube et al., 2017 , 2019 , 2020 ). In contrast, information about aquatic biodiversity and community dynamics of the Usuthu River and its associated floodplain lake (Lake Shokwe) is scarce, with no information on how this compares to that of the Phongolo River and its floodplain wetlands, nor the role of these two rivers in the ecological functioning of Lake Nyamithi.…”