Temporal dynamics and population genetic structure of Fusarium graminearum in the upper Midwestern United States

Liang, Junmin; Xayamongkhon, Henry; Broz, Karen; Dong, Yanhong; McCormick, Susan P.; Abramova, S. L.; Ward, Todd J.; Ma, Zhanhong; Kistler, H. Corby

doi:10.1016/j.fgb.2014.10.002

Cited by 78 publications

(75 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ward et al 37 found significant increases in 3ADON frequency, between 1998 and 2004, in the western Canadian provinces, including Manitoba and Saskatchewan37. Similar temporal trends were reported for the midwestern United States and Canada414243. Contradictory results have also reported.…”

Section: Discussionmentioning

confidence: 63%

See 1 more Smart Citation

Temporal dynamics, population characterization and mycotoxins accumulation of Fusarium graminearum in Eastern China

Qiu

Sun

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Trichothecene genotype composition, mycotoxin production, genetic diversity, and population structure were analyzed, using 185 Fusarium strains collected from wheat (Triticum aestivum L.) throughout the Jiangsu province during 1976, 1983, 1998, 2006, and 2014. The results showed that 3-acetyldeoxynivalenol (3ADON) was consistently the predominant type in this region over 40 years, and the nivalenol (NIV) type has emerged since 1998. Long-term rotation of wheat and rice (Oryza sativa L.), rather than fungicide application, crop fitness, or weather conditions, might be the main cause of this phenomenon. The genetic diversity results from two toxin synthetic genes, Pks4 and Tri10, and variable number of tandem repeat (VNTR) markers revealed the largest variance within the population in 1998, which was also the year with the highest production of mycotoxins. Population differentiation analysis indicated that major temporal population comparisons from the same area were not significantly differentiated. Our results showed that dominant species could maintain genetic stability for a long time, and Pks4 would be of utility in genetic and population studies.Fusarium head blight (FHB) is one of the most destructive and widespread diseases of wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), and other cereals, globally. This disease reduces crop yields, causing extensive economic losses. More importantly, harvested grain quality is often compromised, due to mycotoxins produced by pathogens. Consumption of contaminated grains and products might have pernicious effects on human and animal health 1 . Fusarium graminearum species complex (FGSC) consists of at least 16 phylogenetically distinct species, and is the main causal agent of FHB [2][3][4][5][6][7] . Fusarium asiaticum is the predominant species in Asia 8-12 , while F. graminearum sensu stricto (s. str.) is present in most FHB-occurring areas around the world, especially in America and Europe 3,4,6 . Members of FGSC produce toxic secondary metabolites, among which trichothecene and zearalenone (ZEN) are the most closely monitored due to their high detection rates and strong toxicity. Trichothecene toxins, including deoxynivalenol (DON) and nivalenol (NIV), inhibit eukaryotic protein biosynthesis and cause some human and animal mycotoxicoses 13,14 . FGSC strains have been shown to possess one of three trichothecene genotypes: 3-acetyldeoxynivalenol (3ADON), or NIV 15 . Zearalenone (ZEN) and its metabolites have estrogen-like functions and strong teratogenic effects 16 . Moreover, ZEN has been shown to be genotoxic to cells and toxic to some animal tissues 17 . As a result, the mycotoxin-producing ability of individual isolates is always a concern.With the development of molecular biology, sequences and functions of members in the trichothecene biosynthetic gene cluster have been gradually elucidated. The first gene discovered in the trichothecene pathway was Tri5 18 , which catalyzes the first reaction in this pathway. Thereafter, the other 11 core genes...

show abstract

Section: Discussionmentioning

confidence: 63%

“…Contradictory results have also reported. There were no significant differences in the 3ADON ratios in Alberta, of 1998 vs. 2004 and 2005 vs. 2007, which was not the case in Manitoba and Saskatchewan43.…”

Section: Discussionmentioning

confidence: 66%

Temporal dynamics, population characterization and mycotoxins accumulation of Fusarium graminearum in Eastern China

Qiu

Sun

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Fusarium head blight pathogen surveys of the USA have uncovered an unexpected diversity of trichothecene mycotoxins. In addition to strains that produce the common trichothecenes 15-acetyl,deoxynivalenol (15ADON) and 3-acetyl, deoxynivalenol (3ADON), others have been shown to produce nivalenol (NIV) or the newly described trichothecene toxin NX-2 (Liang et al, 2014;Varga et al, 2015). Notably, F. gerlachii and NX-2-producing strains of F. graminearum s.s. were first discovered growing on symptomless wild grasses in Minnesota, USA (Starkey et al, 2007;Varga et al, 2015), and were only later discovered as FHB pathogens on wheat.…”

Section: Introductionmentioning

confidence: 99%

Fusarium graminearum: pathogen or endophyte of North American grasses?

et al. 2017

View full text Add to dashboard Cite

Summary Mycotoxin‐producing Fusarium graminearum and related species cause Fusarium head blight on cultivated grasses, such as wheat and barley. However, these Fusarium species may have had a longer evolutionary history with North American grasses than with cultivated crops and may interact with the ancestral hosts in ways which are biochemically distinct.We assayed 25 species of asymptomatic native grasses for the presence of Fusarium species and confirmed infected grasses as hosts using re‐inoculation tests. We examined seed from native grasses for the presence of mycotoxin‐producing Fusarium species and evaluated the ability of these fungi to produce mycotoxins in both native grass and wheat hosts using biochemical analysis.Mycotoxin‐producing Fusarium species were shown to be prevalent in phylogenetically diverse native grasses, colonizing multiple tissue types, including seeds, leaves and inflorescence structures. Artificially inoculated grasses accumulated trichothecenes to a much lesser extent than wheat, and naturally infected grasses showed little to no accumulation.Native North American grasses are commonly inhabited by Fusarium species, but appear to accommodate these toxigenic fungi differently from cultivated crops. This finding highlights how host identity and evolutionary history may influence the outcome of plant–fungal interactions and may inform future efforts in crop improvement.

show abstract

“…In addition to homothallic reproduction, the genetic diversity observed between field isolates suggests that F. graminearum outcrosses frequently (Chen and Zhou, 2009;Liang et al, 2014;Talas and McDonald, 2015a). Fungal pathogens that exhibit mixed reproduction can evolve rapidly, thereby endangering the efficiency and durability of control strategies (McDonald and Linde, 2002;Pariaud et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

High‐resolution mapping of the recombination landscape of the phytopathogen Fusarium graminearum suggests two‐speed genome evolution

Laurent

Palaiokostas

Spataro

et al. 2017

Molecular Plant Pathology

View full text Add to dashboard Cite

Recombination is a major evolutionary force, increasing genetic diversity and permitting efficient coevolution of fungal pathogen(s) with their host(s). The ascomycete Fusarium graminearum is a devastating pathogen of cereal crops, and can contaminate food and feed with harmful mycotoxins. Previous studies have suggested a high adaptive potential of this pathogen, illustrated by an increase in pathogenicity and resistance to fungicides. In this study, we provide the first detailed picture of the crossover events occurring during meiosis and discuss the role of recombination in pathogen evolution. An experimental recombinant population (n = 88) was created and genotyped using 1306 polymorphic markers obtained from restriction site-associated DNA sequencing (RAD-seq) and aligned to the reference genome. The construction of a high-density linkage map, anchoring 99% of the total length of the reference genome, allowed the identification of 1451 putative crossovers, positioned at a median resolution of 24 kb. The majority of crossovers (87.2%) occurred in a relatively small portion of the genome (30%). All chromosomes demonstrated recombination-active sections, which had a near 15-fold higher crossover rate than non-active recombinant sections. The recombination rate showed a strong positive correlation with nucleotide diversity, and recombination-active regions were enriched for genes with a putative role in host-pathogen interaction, as well as putative diversifying genes. Our results confirm the preliminary analysis observed in other F. graminearum strains and suggest a conserved 'two-speed' recombination landscape. The consequences with regard to the evolutionary potential of this major fungal pathogen are also discussed.

show abstract

Temporal dynamics and population genetic structure of Fusarium graminearum in the upper Midwestern United States

Cited by 78 publications

References 30 publications

Temporal dynamics, population characterization and mycotoxins accumulation of Fusarium graminearum in Eastern China

Temporal dynamics, population characterization and mycotoxins accumulation of Fusarium graminearum in Eastern China

Fusarium graminearum: pathogen or endophyte of North American grasses?

High‐resolution mapping of the recombination landscape of the phytopathogen Fusarium graminearum suggests two‐speed genome evolution

Contact Info

Product

Resources

About