Temporal Differences in Interneuron Invasion of Neocortex and Piriform Cortex during Mouse Cortical Development

Hsing, Hsiang-Wei; Zhuang, Zi-Hui; Niou, Zhen-Xian; Chou, Shen-Ju

doi:10.1093/cercor/bhz291

Cited by 8 publications

(7 citation statements)

References 76 publications

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“…KCC2 expression during development has been proved to be highly region and species-specific. In the mouse, Hsing and colleagues (Hsing et al 2020) showed that in neocortical areas very low KCC2 expression can be detected at P0, whereas KCC2 signal in the piriform cortex can be detected as early as E15.5. Nonetheless both regions display similar levels of expression by P7.…”

Section: Discussionmentioning

confidence: 99%

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Region-specific KCC2 rescue by rhIGF-1 and oxytocin in a mouse model of Rett syndrome

Gigliucci

Teutsch

Woodbury‐Smith

et al. 2021

Preprint

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Rett syndrome (RTT) is characterized by dysfunction in neuronal excitation/inhibition (E/I) balance, potentially impacting seizure susceptibility via deficits in K+/Cl- cotransporter 2 (KCC2) function. Mice lacking the Methyl-CpG binding protein 2 (MeCP2) recapitulate many symptoms of RTT, and recombinant human insulin-like growth factor-1 (rhIGF-1) restores KCC2 expression and E/I balance in MeCP2 KO mice. However, clinical trial outcomes of rhIGF-1 in RTT have been variable, and increasing its therapeutic efficacy is highly desirable. To this end, the neuropeptide oxytocin (OXT) is promising, as it also critically modulates KCC2 function during early postnatal development. We measured basal KCC2 expression levels in MeCP2 KO mice and identified three key frontal brain regions showing KCC2 alterations in young adult mice but not in postnatal P10 animals. We thus hypothesized that deficits in IGF-1/OXT signaling crosstalk modulating KCC2 may occur in RTT during postnatal development. Consistently, we detected alterations of IGF-1 receptor (IGF-1R) and OXT receptor (OXTR) levels in those brain areas. rhIGF-1 and OXT treatments in KO mice rescued KCC2 expression in a region-specific and complementary manner. These results suggest that region-selective combinatorial pharmacotherapeutic strategies could be the most effective at normalizing E/I balance in key brain regions subtending the RTT pathophysiology.

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Section: Discussionmentioning

confidence: 99%

“…KCC2 expression during development has been proved to be highly region and species-specific. In the mouse, Hsing and colleagues (Hsing et al 2020) showed that in neocortical areas very low KCC2 expression preprint (which was not certified by peer review) is the author/funder. All rights reserved.…”

Section: Circuit-specific Kcc2 Deficiency In Mecp2 Ko Micementioning

confidence: 99%

Region-specific KCC2 rescue by rhIGF-1 and oxytocin in a mouse model of Rett syndrome

Gigliucci

Teutsch

Woodbury‐Smith

et al. 2021

Preprint

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“…Quantitative RT-PCR was performed as described 50 . Briefly, RNA samples were collected from dorsal telencephalon at E13.5 using TriPure Isolation Reagent (Roche).…”

Section: Methodsmentioning

confidence: 99%

“…Cell nuclei were counterstained with DAPI (Vector). Neuronal birthdating analyses were performed as described 50 . Briefly, EdU (500 ng) was injected into timed-pregnant mice, and the EdU-positive cells were detected with a Click-iT EdU imaging kit (Life Technologies).…”

Section: Methodsmentioning

confidence: 99%

Specific contribution of neurons from the Dbx1 lineage to the piriform cortex

Shabangu

Chen

Zhuang

et al. 2021

Sci Rep

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The piriform cortex (PC) is a major cortical processing center for the sense of smell that receives direct inputs from the olfactory bulb. In mice, the PC consists of three neuronal layers, which are populated by cells with distinct developmental origins. One origin of PC neurons is the pool of Dbx1-expressing neural progenitors located in the ventral pallium at the pallial-subpallial boundary. Since the precise mechanisms of PC neuron development are largely unknown, we sought to define the distribution, timing of neurogenesis, morphology and projection patterns of PC neurons from the Dbx1 lineage. We found that Dbx1-lineage neurons are preferentially distributed in layer 2 and enriched in the ventral portion of the PC. Further, Dbx1 neurons are early-born neurons and contribute to most neuronal subtypes in the PC. Our data also revealed an enrichment of Dbx1-lineage neurons in the ventral anterior PC that project to the orbitofrontal cortex. These findings suggest a specific association between the developmental origin of PC neurons and their neuronal properties.

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“…Cell nuclei were counterstained with DAPI (Vector). Neuronal birthdating analyses were performed as described 44 . Briefly, EdU (500 ng) was injected into timed-pregnant mice, and the EdU-positive cells were detected with a Click-iT EdU imaging kit (Life Technologies).…”

Section: Mouse Linesmentioning

confidence: 99%

Specific Contribution of Neurons From the Dbx1 Lineage to The Piriform Cortex

Shabangu

Chen

Zhuang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

The piriform cortex (PC) is a major cortical processing center for the sense of smell that receives direct inputs from the olfactory bulb. In mice, the PC consists of three neuronal layers, which are populated by cells with distinct developmental origins. One of developmental origins of PC is the Dbx1-expressing neural progenitors located in the ventral pallium at the pallial-subpallial boundary. Since the precise mechanisms of PC neuron development are largely unknown, we sought to define the distribution, timing of neurogenesis, morphology and projection patterns of PC neurons from the Dbx1 lineage. We found that Dbx1-lineage neurons are preferentially distributed in layer 2 and enriched in the ventral portion of the PC. Further, Dbx1 neurons are early-born neurons that contribute to most neuronal subtypes in the PC. Our data also revealed an enrichment of Dbx1-lineage neurons located in the ventral anterior PC that project to the orbitofrontal cortex. These findings suggest a specific association between developmental origin of PC neurons and their neuronal properties.

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Temporal Differences in Interneuron Invasion of Neocortex and Piriform Cortex during Mouse Cortical Development

Cited by 8 publications

References 76 publications

Region-specific KCC2 rescue by rhIGF-1 and oxytocin in a mouse model of Rett syndrome

Region-specific KCC2 rescue by rhIGF-1 and oxytocin in a mouse model of Rett syndrome

Specific contribution of neurons from the Dbx1 lineage to the piriform cortex

Specific Contribution of Neurons From the Dbx1 Lineage to The Piriform Cortex

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